ID C0BXF4_9FIRM Unreviewed; 436 AA.
AC C0BXF4;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 24-JAN-2024, entry version 51.
DE RecName: Full=Phenylacetate-coenzyme A ligase {ECO:0000256|PIRNR:PIRNR006444};
DE EC=6.2.1.30 {ECO:0000256|PIRNR:PIRNR006444};
DE AltName: Full=Phenylacetyl-CoA ligase {ECO:0000256|PIRNR:PIRNR006444};
GN ORFNames=CLOHYLEM_04491 {ECO:0000313|EMBL:EEG75261.1};
OS [Clostridium] hylemonae DSM 15053.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=553973 {ECO:0000313|EMBL:EEG75261.1, ECO:0000313|Proteomes:UP000004893};
RN [1] {ECO:0000313|EMBL:EEG75261.1, ECO:0000313|Proteomes:UP000004893}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15053 {ECO:0000313|EMBL:EEG75261.1,
RC ECO:0000313|Proteomes:UP000004893};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Clostridium hylemonae (DSM 15053).";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEG75261.1, ECO:0000313|Proteomes:UP000004893}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15053 {ECO:0000313|EMBL:EEG75261.1,
RC ECO:0000313|Proteomes:UP000004893};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the activation of phenylacetic acid (PA) to
CC phenylacetyl-CoA (PA-CoA). {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-phenylacetate + ATP + CoA = AMP + diphosphate +
CC phenylacetyl-CoA; Xref=Rhea:RHEA:20956, ChEBI:CHEBI:18401,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57390, ChEBI:CHEBI:456215; EC=6.2.1.30;
CC Evidence={ECO:0000256|PIRNR:PIRNR006444};
CC -!- PATHWAY: Aromatic compound metabolism; phenylacetate degradation.
CC {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- SIMILARITY: Belongs to the phenylacetyl-CoA ligase family.
CC {ECO:0000256|PIRNR:PIRNR006444}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEG75261.1}.
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DR EMBL; ABYI02000012; EEG75261.1; -; Genomic_DNA.
DR RefSeq; WP_006441823.1; NZ_GG657759.1.
DR AlphaFoldDB; C0BXF4; -.
DR STRING; 553973.CLOHYLEM_04491; -.
DR eggNOG; COG1541; Bacteria.
DR HOGENOM; CLU_035301_1_1_9; -.
DR OrthoDB; 580775at2; -.
DR UniPathway; UPA00930; -.
DR Proteomes; UP000004893; Unassembled WGS sequence.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0047475; F:phenylacetate-CoA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0010124; P:phenylacetate catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd05913; PaaK; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR028154; AMP-dep_Lig_C.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR011880; PA_CoA_ligase.
DR PANTHER; PTHR43845; BLR5969 PROTEIN; 1.
DR PANTHER; PTHR43845:SF1; BLR5969 PROTEIN; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF14535; AMP-binding_C_2; 1.
DR PIRSF; PIRSF006444; PaaK; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR006444};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR006444}.
FT DOMAIN 78..286
FT /note="AMP-dependent synthetase/ligase"
FT /evidence="ECO:0000259|Pfam:PF00501"
FT DOMAIN 336..432
FT /note="AMP-dependent ligase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14535"
SQ SEQUENCE 436 AA; 48513 MW; 08CB490B45FF5455 CRC64;
MIWAKEETLP RDEIEGIQLE KLKETVRYIY EKSAPYRRKM DEAGVRPDDI GSLTDLQKLP
FTYKADFRDN YPMGLFAVDK KELVRFHASS GTTGKPTVVG YTKKDLDVWL NNVARIACMG
GATPDDVAQI AFGYGTFTGA LGLHGGLEKL GASVIPMSSG NTKKQIMFMQ DMGTTLLVAT
PSYALHLGEE IRARGLDPSK DLKVHIGLFG GEGMTEPMRD EMHKVWGEQF LCTQNYGMSE
LCGPGVAGEC ESLCGMHVNE DWFIPEVIDP ETGDVLPPGE HGELVVTCLG KEALPLVRYR
TGDLTKLMYE PCACGRTTVR MENLSGRADD MLVIRGVNVF PTQIEEVLLQ VPEIGPHYEI
VVERKNRLDV MTITVELIDD RLLDSYSKLS ELEQRIKKGL KAQLGLATQI KLVAPYSLQR
FEGKAKRVTD MRKDGL
//
