ID C0BYN1_9FIRM Unreviewed; 738 AA.
AC C0BYN1;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=CLOHYLEM_04920 {ECO:0000313|EMBL:EEG74959.1};
OS [Clostridium] hylemonae DSM 15053.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=553973 {ECO:0000313|EMBL:EEG74959.1, ECO:0000313|Proteomes:UP000004893};
RN [1] {ECO:0000313|EMBL:EEG74959.1, ECO:0000313|Proteomes:UP000004893}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15053 {ECO:0000313|EMBL:EEG74959.1,
RC ECO:0000313|Proteomes:UP000004893};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Clostridium hylemonae (DSM 15053).";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEG74959.1, ECO:0000313|Proteomes:UP000004893}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15053 {ECO:0000313|EMBL:EEG74959.1,
RC ECO:0000313|Proteomes:UP000004893};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase.
CC {ECO:0000256|PIRNR:PIRNR005536}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEG74959.1}.
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DR EMBL; ABYI02000018; EEG74959.1; -; Genomic_DNA.
DR AlphaFoldDB; C0BYN1; -.
DR STRING; 553973.CLOHYLEM_04920; -.
DR eggNOG; COG3345; Bacteria.
DR HOGENOM; CLU_009640_2_1_9; -.
DR Proteomes; UP000004893; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536}.
FT DOMAIN 41..294
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 660..735
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 490
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 558
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 738 AA; 83411 MW; EF9D650944F91AC8 CRC64;
MTEIRMIKGE GHMAIIYDDR KKTFTLNTES TTYQMQVDRY GFLLHLYYGK LAEGCMDYVL
TYYDRGFSGN PYEAGKDRTY SLDALPQEFP CRGNGDFRAA ALTVRNADGT YCCDMRYRSH
VIVKGKYDLP GLPAVYGTEQ EAQTLEITME DSASGLQAVL LYGVLPELDI ITRSVRIVNA
GEKKVYIENI QSACLDLLSG NYDVISFYGR HAMERNFQRI PVPHGSFRIG SRRGTSSHQY
SPFMILADKN ATEDAGSCYA MNFVYSGGFS GEAEKDQFNQ TRMMMGLQDE LFSYPAAPGE
TFQAPEVILS YTDKGLNRLS QHMHRCIRTH VCRGKYRDAV RPVLVNSWEA AYFDFTGETI
RKLAEDAKEC GIDMVVMDDG WFGSRDGDFS GLGDWHVNEE KLGGTLRDLI RQINDMGIKF
GIWIEPEMVN EDSDLYRAHP DWVLSVPGRK PVRARYQLVL DFSRKEVVDA LFEQICKILD
QGNIEYVKWD MNRSLSDVYS AGTEEQGRVL YDYVLGVYDF LERLTARYPD LLIEGCSGGG
GRFDAGMMYY TPQIWCSDNT DAVDRVKIQY GSSFGYPVSA VGAHVSASPN HQTGRRTPMY
TRTVTAMAGT FGYELNLGAL SGEEKKEIRQ QTDLYRKYAP LIQNGTYYRL SNPYEDEIGA
WAFVSEDRRK VLLNAVTLDV HGNMTVNYIK PKGLKEDAVY EDMSSGQRYC GAALMEAGIP
LPAAACEYEA YQIYLEEV
//
