ID C0EIV6_9FIRM Unreviewed; 1704 AA.
AC C0EIV6;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=alpha-L-rhamnosidase {ECO:0000256|ARBA:ARBA00012652};
DE EC=3.2.1.40 {ECO:0000256|ARBA:ARBA00012652};
GN ORFNames=CLOSTMETH_03801 {ECO:0000313|EMBL:EEG28617.1};
OS [Clostridium] methylpentosum DSM 5476.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Oscillospiraceae incertae sedis.
OX NCBI_TaxID=537013 {ECO:0000313|EMBL:EEG28617.1, ECO:0000313|Proteomes:UP000003340};
RN [1] {ECO:0000313|EMBL:EEG28617.1, ECO:0000313|Proteomes:UP000003340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5476 {ECO:0000313|EMBL:EEG28617.1,
RC ECO:0000313|Proteomes:UP000003340};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEG28617.1, ECO:0000313|Proteomes:UP000003340}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 5476 {ECO:0000313|EMBL:EEG28617.1,
RC ECO:0000313|Proteomes:UP000003340};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Clostridium methylpentosum (DSM 5476).";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing alpha-L-rhamnose residues
CC in alpha-L-rhamnosides.; EC=3.2.1.40;
CC Evidence={ECO:0000256|ARBA:ARBA00001445};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEG28617.1}.
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DR EMBL; ACEC01000130; EEG28617.1; -; Genomic_DNA.
DR STRING; 537013.CLOSTMETH_03801; -.
DR eggNOG; COG1196; Bacteria.
DR eggNOG; COG3408; Bacteria.
DR HOGENOM; CLU_002926_3_1_9; -.
DR Proteomes; UP000003340; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0030596; F:alpha-L-rhamnosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 1.20.1270.90; AF1782-like; 3.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 2.60.420.10; Maltose phosphorylase, domain 3; 1.
DR InterPro; IPR010496; 3-keto-disaccharide_hydrolase.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR035396; Bac_rhamnosid6H.
DR InterPro; IPR035398; Bac_rhamnosid_C.
DR InterPro; IPR013737; Bac_rhamnosid_N.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR008902; Rhamnosid_concanavalin.
DR PANTHER; PTHR33307; ALPHA-RHAMNOSIDASE (EUROFUNG); 1.
DR PANTHER; PTHR33307:SF6; ALPHA-RHAMNOSIDASE (EUROFUNG)-RELATED; 1.
DR Pfam; PF06439; 3keto-disac_hyd; 2.
DR Pfam; PF05592; Bac_rhamnosid; 1.
DR Pfam; PF17389; Bac_rhamnosid6H; 1.
DR Pfam; PF17390; Bac_rhamnosid_C; 1.
DR Pfam; PF08531; Bac_rhamnosid_N; 1.
DR Pfam; PF07554; FIVAR; 3.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 4: Predicted;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023001};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023001};
KW Reference proteome {ECO:0000313|Proteomes:UP000003340};
KW Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..32
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 33..1704
FT /note="alpha-L-rhamnosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002897454"
FT TRANSMEM 1681..1699
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 305..456
FT /note="3-keto-disaccharide hydrolase"
FT /evidence="ECO:0000259|Pfam:PF06439"
FT DOMAIN 502..640
FT /note="3-keto-disaccharide hydrolase"
FT /evidence="ECO:0000259|Pfam:PF06439"
FT DOMAIN 704..850
FT /note="Bacterial alpha-L-rhamnosidase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08531"
FT DOMAIN 878..979
FT /note="Alpha-L-rhamnosidase concanavalin-like"
FT /evidence="ECO:0000259|Pfam:PF05592"
FT DOMAIN 989..1332
FT /note="Alpha-L-rhamnosidase six-hairpin glycosidase"
FT /evidence="ECO:0000259|Pfam:PF17389"
FT DOMAIN 1342..1414
FT /note="Alpha-L-rhamnosidase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF17390"
SQ SEQUENCE 1704 AA; 183165 MW; 02F30C10CFF267BB CRC64;
MMLKQGMKRI LALVLSVAVA GAVLVSPLSA SAQQGTTAPG APYGLLTNEL EHPLNVETPT
FSWWLEDADV DEGQTAYQLV VRDELTGEVV WDSGKVESSE QSNVPYGGDA LSPAHPYTWT
VTTWDSKGLQ SPESEPAAFA TGLADADWNA DWIQKPNLPI TVESSGAAAH VEGGGLTIYS
EGTDWADYKM NVEACAKQGA AGIVLRAQDE DNAYIWALVP GTGLTKTALV NGEAKDLGTV
AFDVVEGAYY SVEIVADGST ITTKIDGQLI DTTTDSTFSA GTVGFYEADG QAGDFQKLSV
TSLARVVDSI YVAGGSNENS QIVLNDKGYD WSDYILDFDM QINDTSNDAT ALVLRASSDY
KTGYMWQFTR KKGGLARHTR TNGKFNKLDG DGAVPYSFKA NTYYHITISL IGNTITTSVN
GEVIDTYVDT ANVASKGTFG FRNASGEGGT YANLKVTAPD GTPIYTEDFL DLSRFKFLDG
NAANTITAKD NTVTYLSPDF SKGFEGWSNS SSMSTADGSN WATSTSSGIT LAKTGKDWDN
YTLTTRIIAK TGAAGVMFRT TDAQSGYMWQ IVPGTGLKVH KQVNGKFTTL KNPIPCDIKA
GQTYRMTINA NGSVIKTYIN GQLVDITDDN TYATGTVGFR QSTTGNEVGQ FSDVMVLDAS
GEVLFQDNFT NGLGQWDIST TTAAKGSNLY WYARTEQPLE EGKTPVRALA YMAAAHDYEL
SVNGTRIGRG QSFDYSSETH YQGWDITEAL DGSGTIAVGV LARWYGNGQG RAGGTQGLLG
QIVVYYDDGS TQVISTGKDW VAEEAPLSGS TKRNGEGDFV EEYDARKAIA DYSAVGLDVS
GWMPVHVLGA HPADPFTNVE PELSHVTEET MSPVSVEKLS DGTTLVDFGR VIPARLSVDF
KNGVAGRKIT LQTGYELKDN GRINTSSSAT QSTNMTFIYT QTEGAQRYDT WDHLAFRYLE
IPASAGETFT VQDITATLLY AEVPEGRDST FTSSNEMLDA VYDFMKISAL YSAQNQFVDT
PTREKGQFLQ DSINIGAVTT TSWYERETSR KAIRQFLDSA DRYWNSGGDL GHYNSVYPNG
DGKRDIPDFS LNVPIWIWRY YQQTGDREIL EYAYPYLQNT ADYAYNAIPT EGATAGLVTT
LPGGSGDYKN GIVDWPKPGR FDYDMSTAAR TTVNALSVRV FDTVAEIAKE LGKDDSEVAV
YADRAANLRQ AMNEKLITED GVYCDGLLSD GTQSKHNGQH STSYAIAFDI APADKVEAMA
DYVASLGMKQ GPMTADILVE ALFDADRADA VLNLMTNTDD LGWAKLIADG NTFTWEQWVH
GQSQSHGWGA ASAPLILENF LGVKVTTAGA KTVTIDPARD VLEHAAGRVV TERGAVDVSY
SGTGTDYTLK VTVPVNMQAE VVLPKLEGGQ FVDKNGNSGT SSFTEDEQIV TVGSGTREFV
FQTYVDANKA ILNAVISYAQ EQKDSAEFDN VIAQVQATFT EALDHAIAVA DSQFATQDEV
DAAWKALMNE IHKLGFVKGD ISSLETLVQA AEDIDLTLYV EKGQAEFTAA LQAAQAVVAD
KDNAMEQEIQ EATDRLLDAM LELRVKADKS ILQALLADAA NVDVSLYTAE TVATFQAAND
AAKDIYDNPD ATQAEVNDAA QALQDAISGL KSADTSAQGA AVQGDANATT GSGNAKTGET
VPVAAAAALL LAGAAAIVFK KRLK
//
