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Database: UniProt
Entry: C0H419
LinkDB: C0H419
Original site: C0H419 
ID   BLAP_BACSU              Reviewed;          73 AA.
AC   C0H419; Q9R9I3;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   10-APR-2019, entry version 51.
DE   RecName: Full=Biotin/lipoyl attachment protein;
DE            Short=BLAP;
GN   Name=yngHB; OrderedLocusNames=BSU18239;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX   PubMed=10557314; DOI=10.1073/pnas.96.23.13294;
RA   Duitman E.H., Hamoen L.W., Rembold M., Venema G., Seitz H.,
RA   Saenger W., Bernhard F., Reinhardt R., Schmidt M., Ullrich C.,
RA   Stein T., Leenders F., Vater J.;
RT   "The mycosubtilin synthetase of Bacillus subtilis ATCC6633: a
RT   multifunctional hybrid between a peptide synthetase, an amino
RT   transferase, and a fatty acid synthase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:13294-13299(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   STRUCTURE BY NMR OF 2-73 OF THE APO- AND BIOTINYLATED FORMS,
RP   BIOTINYLATION AT LYS-35, LIPOYLATION AT LYS-35, INDUCTION, MASS
RP   SPECTROMETRY, AND MUTAGENESIS OF TRP-12.
RC   STRAIN=168;
RX   PubMed=16699181; DOI=10.1074/jbc.M602660200;
RA   Cui G., Nan B., Hu J., Wang Y., Jin C., Xia B.;
RT   "Identification and solution structures of a single domain
RT   biotin/lipoyl attachment protein from Bacillus subtilis.";
RL   J. Biol. Chem. 281:20598-20607(2006).
CC   -!- INDUCTION: Detected in log-phase B.subtilis cells (at protein
CC       level). {ECO:0000269|PubMed:16699181}.
CC   -!- PTM: Can be both biotinylated and lipoylated on Lys-35 upon
CC       overexpression in E.coli depending on the growth medium; the
CC       nature of the modification in situ in B.subtilis is unknown.
CC       {ECO:0000269|PubMed:16699181}.
CC   -!- MASS SPECTROMETRY: Mass=7913; Method=Unknown; Range=2-73;
CC       Note=Upon overexpression in E.coli.;
CC       Evidence={ECO:0000269|PubMed:16699181};
DR   EMBL; AF184956; AAF08803.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAX52627.1; -; Genomic_DNA.
DR   PIR; T44814; T44814.
DR   RefSeq; WP_003245519.1; NZ_JNCM01000035.1.
DR   RefSeq; YP_003097733.1; NC_000964.3.
DR   PDB; 1Z6H; NMR; -; A=2-73.
DR   PDB; 1Z7T; NMR; -; A=2-73.
DR   PDB; 2B8F; NMR; -; A=2-73.
DR   PDB; 2B8G; NMR; -; A=2-73.
DR   PDBsum; 1Z6H; -.
DR   PDBsum; 1Z7T; -.
DR   PDBsum; 2B8F; -.
DR   PDBsum; 2B8G; -.
DR   ProteinModelPortal; C0H419; -.
DR   SMR; C0H419; -.
DR   STRING; 224308.BSU18239; -.
DR   PaxDb; C0H419; -.
DR   PRIDE; C0H419; -.
DR   EnsemblBacteria; CAX52627; CAX52627; BSU18239.
DR   GeneID; 8303012; -.
DR   KEGG; bsu:BSU18239; -.
DR   PATRIC; fig|224308.179.peg.1989; -.
DR   eggNOG; ENOG4106063; Bacteria.
DR   eggNOG; COG0511; LUCA.
DR   HOGENOM; HOG000020245; -.
DR   InParanoid; C0H419; -.
DR   OMA; MAGNLWK; -.
DR   PhylomeDB; C0H419; -.
DR   BioCyc; BSUB:BSU18239-MONOMER; -.
DR   EvolutionaryTrace; C0H419; -.
DR   Proteomes; UP000001570; Chromosome.
DR   InterPro; IPR000089; Biotin_lipoyl.
DR   InterPro; IPR011053; Single_hybrid_motif.
DR   Pfam; PF00364; Biotin_lipoyl; 1.
DR   SUPFAM; SSF51230; SSF51230; 1.
DR   PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Biotin; Complete proteome; Lipoyl; Reference proteome.
FT   INIT_MET      1      1       Removed. {ECO:0000255}.
FT   CHAIN         2     73       Biotin/lipoyl attachment protein.
FT                                /FTId=PRO_0000389494.
FT   DOMAIN        2     69       Biotinyl-binding. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01066}.
FT   REGION        2     68       Biotinyl-binding and/or lipoyl-binding.
FT   MOD_RES      35     35       N6-biotinyllysine; alternate.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01066,
FT                                ECO:0000269|PubMed:16699181}.
FT   MOD_RES      35     35       N6-lipoyllysine; alternate.
FT                                {ECO:0000269|PubMed:16699181}.
FT   VARIANT       4      4       S -> I (in strain: ATCC 6633).
FT   VARIANT      21     21       Q -> H (in strain: ATCC 6633).
FT   VARIANT      47     47       I -> T (in strain: ATCC 6633).
FT   VARIANT      54     54       K -> N (in strain: ATCC 6633).
FT   VARIANT      70     70       N -> D (in strain: ATCC 6633).
FT   MUTAGEN      12     12       W->M: No changes in biotinylation in
FT                                E.coli. {ECO:0000269|PubMed:16699181}.
FT   STRAND        7     14       {ECO:0000244|PDB:1Z6H}.
FT   STRAND       27     33       {ECO:0000244|PDB:1Z6H}.
FT   STRAND       36     41       {ECO:0000244|PDB:1Z6H}.
FT   STRAND       46     53       {ECO:0000244|PDB:1Z6H}.
FT   STRAND       64     68       {ECO:0000244|PDB:1Z6H}.
FT   HELIX        69     71       {ECO:0000244|PDB:1Z6H}.
SQ   SEQUENCE   73 AA;  8041 MW;  0358FB92D5DBED91 CRC64;
     MTVSIQMAGN LWKVHVKAGD QIEKGQEVAI LESMKMEIPI VADRSGIVKE VKKKEGDFVN
     EGDVLLELSN STQ
//
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