ID C0NM44_AJECG Unreviewed; 612 AA.
AC C0NM44;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE SubName: Full=tRNA pseudouridine synthase {ECO:0000313|EMBL:EEH07695.1};
GN ORFNames=HCBG_04574 {ECO:0000313|EMBL:EEH07695.1};
OS Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432)
OS (Darling's disease fungus) (Histoplasma capsulatum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma.
OX NCBI_TaxID=447093 {ECO:0000313|EMBL:EEH07695.1, ECO:0000313|Proteomes:UP000001631};
RN [1] {ECO:0000313|Proteomes:UP000001631}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G186AR / H82 / ATCC MYA-2454 / RMSCC 2432
RC {ECO:0000313|Proteomes:UP000001631};
RA Champion M., Cuomo C.A., Ma L.-J., Henn M.R., Sil A., Goldman B.,
RA Young S.K., Kodira C.D., Zeng Q., Koehrsen M., Alvarado L., Berlin A.,
RA Borenstein D., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J.,
RA Griggs A., Gujja S., Heiman D., Hepburn T., Howarth C., Jen D., Larson L.,
RA Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T.,
RA Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C.,
RA Klein B., McEwen J.G., Puccia R., Goldman G.H., Felipe M.S., Nino-Vega G.,
RA San-Blas G., Taylor J., Mendoza L., Galagan J.E., Nusbaum C., Birren B.W.;
RT "The genome sequence of Ajellomyces capsulatus strain G186AR.";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; GG663367; EEH07695.1; -; Genomic_DNA.
DR AlphaFoldDB; C0NM44; -.
DR STRING; 447093.C0NM44; -.
DR VEuPathDB; FungiDB:I7I50_11172; -.
DR HOGENOM; CLU_021971_1_0_1; -.
DR InParanoid; C0NM44; -.
DR OrthoDB; 12038at2759; -.
DR Proteomes; UP000001631; Unassembled WGS sequence.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000001631};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 399..504
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 1..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 311..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 575..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..36
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..93
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..329
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..363
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 180
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 236
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 612 AA; 68519 MW; D50ABBE5F983AC2C CRC64;
MDTQQQSPNP NRPSNSAPDQ QAMFPAGYSQ EQEVSQPRPD GDAKRKKEPN SGGGGRGGRK
NKRKDLGRKA WSRQIPDKRA RAEASQESKR RKLENGEKAL PIYATEFSKE DIEADQRRPK
KKVAVMLGYS GSGYKGMQLS ETEKTIEGDL FTAFVAAGAI SKANATDPKK SSLVRCARTD
KGVHAAGNVV SLKLIVEDPN VVQKINGHLS PQIRVWGIEI TNKSFSSYHL CDSRIYEYLI
PSHCFLPPHP STHLGQQLVE IAEQENDLEG YQQRQAEVAH YWEEVDEKYI KPILERLPEV
TRNRVKQALI IKSGTDPADN STSQGEQDQL DPSDEQDGEV TSKEASASTN ENKDLSSGDE
QTVHTTNEAI RIIRAAYLGA KKAYRIPRER MDRINDALGM YVGTRNFHNY TIQKSFRDAS
AKRHIKSFKI SREPLIINGT EWLSLKVHGQ SFMMHQIRKM VAMAALIVRC GCDIKLVPET
YGDQKIAIPK APGLGLLLER PIFDSYNKRT VVEYGKNPID FSKHEKEIEE FKQREIYERI
FREEEESNSF GNFFNHIDTF QDNAFLFVTS RGIPASKPSG GSAKAPMSGK AALKEVESES
DDDLVNGGEE GG
//
