ID C0QCH3_DESAH Unreviewed; 515 AA.
AC C0QCH3;
DT 05-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 05-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN Name=hsdM2 {ECO:0000313|EMBL:ACN15050.1};
GN OrderedLocusNames=HRM2_19490 {ECO:0000313|EMBL:ACN15050.1};
OS Desulforapulum autotrophicum (strain ATCC 43914 / DSM 3382 / VKM B-1955 /
OS HRM2) (Desulfobacterium autotrophicum).
OC Bacteria; Thermodesulfobacteriota; Desulfobacteria; Desulfobacterales;
OC Desulfobacteraceae; Desulforapulum.
OX NCBI_TaxID=177437 {ECO:0000313|EMBL:ACN15050.1, ECO:0000313|Proteomes:UP000000442};
RN [1] {ECO:0000313|EMBL:ACN15050.1, ECO:0000313|Proteomes:UP000000442}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43914 / DSM 3382 / HRM2
RC {ECO:0000313|Proteomes:UP000000442};
RX PubMed=19187283; DOI=10.1111/j.1462-2920.2008.01825.x;
RA Strittmatter A.W., Liesegang H., Rabus R., Decker I., Amann J., Andres S.,
RA Henne A., Fricke W.F., Martinez-Arias R., Bartels D., Goesmann A.,
RA Krause L., Puehler A., Klenk H.P., Richter M., Schuler M., Gloeckner F.O.,
RA Meyerdierks A., Gottschalk G., Amann R.;
RT "Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate
RT reducer oxidizing organic carbon completely to carbon dioxide.";
RL Environ. Microbiol. 11:1038-1055(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP001087; ACN15050.1; -; Genomic_DNA.
DR RefSeq; WP_015903831.1; NC_012108.1.
DR AlphaFoldDB; C0QCH3; -.
DR SMR; C0QCH3; -.
DR STRING; 177437.HRM2_19490; -.
DR REBASE; 20389; M.DauHRMORF19490P.
DR KEGG; dat:HRM2_19490; -.
DR eggNOG; COG0286; Bacteria.
DR HOGENOM; CLU_013049_4_2_7; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000000442; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:InterPro.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ACN15050.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000442};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ACN15050.1}.
FT DOMAIN 8..135
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 148..480
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 515 AA; 57688 MW; 32A14108B72924C5 CRC64;
MTLDLPSLET WLWGSADILR GSIDSSDFKN YIFGLLFLKR ANDVFDEENE KLVEKENWDI
EAAASDPDYH KFFIPDTARW QTIIEKTENI GQAIDEALAA IEEENLNLEG VMTAVHFGNK
DVLSDALLQR LLNHFNKYSL KNKDLYTPDL LGDAYEYLIK MFADDAGKKG GEFYTPKGVV
RLIVQLIKPE PKNSVYDPTC GSGGMLVESA RYIAEQGGKV GELLDASLFG QEKNLGTWAI
CKINMILHNY SDADIKKGCT LSTPKHSTSD GELMIFDRVI ANPPFSQNKW WDAAEVDVKV
NGNGKEMAVN YSKAVSDPYG RFQYGVPPRG YADLAFLQHM ISVLNQNGKL GIVLPHGVLF
RGGSEGKIRK GILKDDILEA VVGLPSKLFY NTGIPASILI VNKSKPIHLK NKVIFIDASQ
DYKEGKNQNR LEEEHVKKVV EAYDAGQEID KFMRIVDMKE IKENDYNLNI TRYIDTSEEE
VAVDLVATLA GIKEIEAREQ EIDARLAGYL KELGL
//
