ID C0W389_9ACTO Unreviewed; 1110 AA.
AC C0W389;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 24-JAN-2024, entry version 69.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN ORFNames=HMPREF0058_0333 {ECO:0000313|EMBL:EEH66806.1};
OS Actinomyces urogenitalis DSM 15434.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Actinomyces.
OX NCBI_TaxID=525246 {ECO:0000313|EMBL:EEH66806.1, ECO:0000313|Proteomes:UP000004778};
RN [1] {ECO:0000313|EMBL:EEH66806.1, ECO:0000313|Proteomes:UP000004778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15434 {ECO:0000313|EMBL:EEH66806.1,
RC ECO:0000313|Proteomes:UP000004778};
RA Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., Chaboub L.,
RA Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., Fowler G.,
RA Francisco L., Fu Q., Gubbala S., Hale W., Han Y., Hemphill L.,
RA Highlander S.K., Hirani K., Hogues M., Jackson L., Jakkamsetti A.,
RA Javaid M., Jiang H., Korchina V., Kovar C., Lara F., Lee S., Mata R.,
RA Mathew T., Moen C., Morales K., Munidasa M., Nazareth L., Ngo R.,
RA Nguyen L., Okwuonu G., Ongeri F., Patil S., Petrosino J., Pham C., Pham P.,
RA Pu L.-L., Puazo M., Raj R., Reid J., Rouhana J., Saada N., Shang Y.,
RA Simmons D., Thornton R., Warren J., Weissenberger G., Zhang J., Zhang L.,
RA Zhou C., Zhu D., Muzny D., Worley K., Gibbs R.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEH66806.1}.
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DR EMBL; ACFH01000015; EEH66806.1; -; Genomic_DNA.
DR RefSeq; WP_006549637.1; NZ_DS999576.1.
DR AlphaFoldDB; C0W389; -.
DR STRING; 103621.GCA_001067145_01539; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_4_0_11; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000004778; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR010994; RuvA_2-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
DR SUPFAM; SSF47781; RuvA domain 2-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902}; Reference proteome {ECO:0000313|Proteomes:UP000004778};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1110 AA; 119861 MW; 6960BA6F34396A3F CRC64;
MFESSYAELH SHSAYSFLDG ANEPEDLAAA AAELGLTALA LTDHDGMPGI VRHAQAGRAL
GLPTVHGTEL TMSDGAHLPV LARSPEGYRH LVSAISQHNL QAGRRQEPAH RLEDLAQALR
GGTGMAAPSS CLVLTGTARG PLRRALGDPA RPHTWDTQAA DICLGRMVEL LGADGVAVEI
TLNGGPTDAA LTRMLTRLAA SHRLPVLATG AVRCARPADS RLADVLTATR LGTDLEGARG
HLPAIGRWLR SGAEMARLHR RAPQAVGVTA ELAADLAFDL ALVAPDLPAA QVPPGHTPAS
WLRELTRQGA LLRYGTREQD PHAWQVLDHE LEVIESLGFP GYFLIVRSIV EFCEESGILC
QGRGSAANSA VCYALGITAV DAVRHKMLFE RFLSPGRAGY PDIDLDIEAC RREEVIQHVY
TRYGRHRAAQ VANVISYRPR SAVRDAARAL GYPAGLQDAW ARSIEHGWSR EQDGQAREDA
VPEQVLDVAD RLLRLPRHLG VHSGGMVLAA QPVTEVCPVR WAAMEGRSVL QWDKDDCAAA
GLVKFDLLGL GMLTALRLAF TSLGDRGEKV PDLTSRQGDG ILRPAQVGRP WGLHTLPEED
PAVYRLLQAA DTIGVFQVES RAQMATLPRL RPRVFYDIVV EVALIRPGPI QGDAVNPYIR
RRLGREEPDY LHDLLRPALE KTLGVPLFQE QLMQIAVDAA GFSPVEADAL RQAMGAKRSV
ERMEALHLRL VEGMKERGIS QGTAELVFDK LRAFADFGFP ESHAFSFAYL VYASAWLKVR
RPEDFYAGVL AAQPMGFWSP QTLVADARRH GVQVLAADVS HSEVQASVET VRPGAPSQAC
VGGAGEEAST ALTHLDTHRD LAVRLGLAPI KGIGRELAER IVTERQSHGA YRDVADLARR
VRLGRKHLET LAAAGALASL GVSRRQALWS AGVLADEHGR PHLGHRTGGD RTPWCQPPLP
GTAVGSRAPD LPEMSAQERH VADLRLTGVS TETHPLAFLR TALAEDGVVE VVRVGQQGNG
AQVRVAGVVT HRQRPYTAAG TVFLNIEDET GLLNVICSSG MWKRYRHVGR RATAVVVWGV
VESADGVTAL RAQSLQGLEG VVSTGSRDWA
//
