ID C0W460_9ACTO Unreviewed; 410 AA.
AC C0W460;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=Putative 23S rRNA (Uracil-5-)-methyltransferase RumB {ECO:0000313|EMBL:EEH66482.1};
GN ORFNames=HMPREF0058_0654 {ECO:0000313|EMBL:EEH66482.1};
OS Actinomyces urogenitalis DSM 15434.
OC Bacteria; Actinomycetota; Actinomycetes; Actinomycetales; Actinomycetaceae;
OC Actinomyces.
OX NCBI_TaxID=525246 {ECO:0000313|EMBL:EEH66482.1, ECO:0000313|Proteomes:UP000004778};
RN [1] {ECO:0000313|EMBL:EEH66482.1, ECO:0000313|Proteomes:UP000004778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15434 {ECO:0000313|EMBL:EEH66482.1,
RC ECO:0000313|Proteomes:UP000004778};
RA Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., Chaboub L.,
RA Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., Fowler G.,
RA Francisco L., Fu Q., Gubbala S., Hale W., Han Y., Hemphill L.,
RA Highlander S.K., Hirani K., Hogues M., Jackson L., Jakkamsetti A.,
RA Javaid M., Jiang H., Korchina V., Kovar C., Lara F., Lee S., Mata R.,
RA Mathew T., Moen C., Morales K., Munidasa M., Nazareth L., Ngo R.,
RA Nguyen L., Okwuonu G., Ongeri F., Patil S., Petrosino J., Pham C., Pham P.,
RA Pu L.-L., Puazo M., Raj R., Reid J., Rouhana J., Saada N., Shang Y.,
RA Simmons D., Thornton R., Warren J., Weissenberger G., Zhang J., Zhang L.,
RA Zhou C., Zhu D., Muzny D., Worley K., Gibbs R.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEH66482.1}.
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DR EMBL; ACFH01000044; EEH66482.1; -; Genomic_DNA.
DR RefSeq; WP_006547621.1; NZ_DS999574.1.
DR AlphaFoldDB; C0W460; -.
DR STRING; 103621.GCA_001067145_01514; -.
DR GeneID; 81708784; -.
DR eggNOG; COG2265; Bacteria.
DR HOGENOM; CLU_014689_0_0_11; -.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000004778; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 2.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS01230; TRMA_1; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000004778};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT ACT_SITE 366
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 366
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 238
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 271
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 292
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 339
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 410 AA; 43951 MW; C1DC1D99DB3EBAD5 CRC64;
MTATGPSAAF CQRHRAGTCH SCPHQDLPLA EQLALKQARV AALLADGAAA LSPQVWQEPV
ASAASRFRNK AKMVVSGTVQ APVLGILGPQ GRGIDLRACP LHVEAIEEAL PVLARTVTRL
GLVPYDVSAR RGELKHLLVT ASPDGDLMVR WVLRSRRHLE VLRDELPRLR EELETLAVMS
VNLQPVHQAI IEGEEEIVLT TEPGGDRLLM RLELPEQAGP SADHRDLPLL LPTQSFFQTN
TAVAEQLYAT AARWAGDLDT GSRPAQVWDL FCGVGGFALA LAGPGRQVLG VEVSAPAIDG
ARQAARLMGL DAQAARFEAG DARVLDPGAG AVPDLLVLNP PRRGIGQDLA ARIESAGVER
VLYSSCNPAS LARDLEAMSS YTAVRAQLFD MFPHTDHAEV LIELRRSPSA
//
