ID C1E386_MICCC Unreviewed; 310 AA.
AC C1E386;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=tRNA-uridine aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00012386};
DE EC=2.5.1.25 {ECO:0000256|ARBA:ARBA00012386};
GN ORFNames=MICPUN_99893 {ECO:0000313|EMBL:ACO62889.1};
OS Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) (Picoplanktonic
OS green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC Mamiellaceae; Micromonas.
OX NCBI_TaxID=296587 {ECO:0000313|EMBL:ACO62889.1, ECO:0000313|Proteomes:UP000002009};
RN [1] {ECO:0000313|EMBL:ACO62889.1, ECO:0000313|Proteomes:UP000002009}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RCC299 / NOUM17 {ECO:0000313|Proteomes:UP000002009};
RX PubMed=19359590; DOI=10.1126/science.1167222;
RA Worden A.Z., Lee J.H., Mock T., Rouze P., Simmons M.P., Aerts A.L.,
RA Allen A.E., Cuvelier M.L., Derelle E., Everett M.V., Foulon E.,
RA Grimwood J., Gundlach H., Henrissat B., Napoli C., McDonald S.M.,
RA Parker M.S., Rombauts S., Salamov A., Von Dassow P., Badger J.H.,
RA Coutinho P.M., Demir E., Dubchak I., Gentemann C., Eikrem W., Gready J.E.,
RA John U., Lanier W., Lindquist E.A., Lucas S., Mayer K.F., Moreau H.,
RA Not F., Otillar R., Panaud O., Pangilinan J., Paulsen I., Piegu B.,
RA Poliakov A., Robbens S., Schmutz J., Toulza E., Wyss T., Zelensky A.,
RA Zhou K., Armbrust E.V., Bhattacharya D., Goodenough U.W., Van de Peer Y.,
RA Grigoriev I.V.;
RT "Green evolution and dynamic adaptations revealed by genomes of the marine
RT picoeukaryotes Micromonas.";
RL Science 324:268-272(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-
CC amino-3-carboxypropyl]uridine in tRNA + H(+) + S-methyl-5'-
CC thioadenosine; Xref=Rhea:RHEA:62432, Rhea:RHEA-COMP:13339, Rhea:RHEA-
CC COMP:16092, ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:82930; EC=2.5.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00024168};
CC -!- SIMILARITY: Belongs to the TDD superfamily. DTWD2 family.
CC {ECO:0000256|ARBA:ARBA00034489}.
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DR EMBL; CP001325; ACO62889.1; -; Genomic_DNA.
DR RefSeq; XP_002501631.1; XM_002501585.1.
DR AlphaFoldDB; C1E386; -.
DR STRING; 296587.C1E386; -.
DR GeneID; 8242916; -.
DR KEGG; mis:MICPUN_99893; -.
DR eggNOG; KOG4382; Eukaryota.
DR InParanoid; C1E386; -.
DR OrthoDB; 206884at2759; -.
DR Proteomes; UP000002009; Chromosome 4.
DR GO; GO:0016432; F:tRNA-uridine aminocarboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR InterPro; IPR005636; DTW.
DR InterPro; IPR039262; DTWD2/TAPT.
DR PANTHER; PTHR21392:SF0; TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2; 1.
DR PANTHER; PTHR21392; UNCHARACTERIZED; 1.
DR Pfam; PF03942; DTW; 1.
DR SMART; SM01144; DTW; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002009};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 24..275
FT /note="DTW"
FT /evidence="ECO:0000259|SMART:SM01144"
FT REGION 279..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 310 AA; 31781 MW; 35C1D4AF23C65517 CRC64;
MADLEALWAD VADDDAVDVR AAGGRAVCEG CARPASACLC DRLPAQPVET RGALVVLTHP
NESKRALNTG WILPRCMRRC EIVRGRYPPQ HLQDRMAGMA PDVPMYLLYP STSATNLDEV
SVVDDAAAIV SASTGQVSLG PDDVLAGAAY VCVCIDSTWG QAREMAVPTL EKMPARTRLI
QLPLTSTMDD DVDGSRVDAP GLRRFSEGDG GAGSLLTPPA PGAMLTAEAA ARAVAMLEST
CPGGGGGGGG GGGGGGASVE RWCVGAVSAM AGFQARHDPA MSGGAVKRGG GRRQRLADPS
RLEGAAGRAL
//
