ID C1EIE0_MICCC Unreviewed; 788 AA.
AC C1EIE0;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=Glycoside hydrolase family 13 protein {ECO:0000313|EMBL:ACO67665.1};
GN Name=ISA3 {ECO:0000313|EMBL:ACO67665.1};
GN ORFNames=MICPUN_105010 {ECO:0000313|EMBL:ACO67665.1};
OS Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) (Picoplanktonic
OS green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC Mamiellaceae; Micromonas.
OX NCBI_TaxID=296587 {ECO:0000313|EMBL:ACO67665.1, ECO:0000313|Proteomes:UP000002009};
RN [1] {ECO:0000313|EMBL:ACO67665.1, ECO:0000313|Proteomes:UP000002009}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RCC299 / NOUM17 {ECO:0000313|Proteomes:UP000002009};
RX PubMed=19359590; DOI=10.1126/science.1167222;
RA Worden A.Z., Lee J.H., Mock T., Rouze P., Simmons M.P., Aerts A.L.,
RA Allen A.E., Cuvelier M.L., Derelle E., Everett M.V., Foulon E.,
RA Grimwood J., Gundlach H., Henrissat B., Napoli C., McDonald S.M.,
RA Parker M.S., Rombauts S., Salamov A., Von Dassow P., Badger J.H.,
RA Coutinho P.M., Demir E., Dubchak I., Gentemann C., Eikrem W., Gready J.E.,
RA John U., Lanier W., Lindquist E.A., Lucas S., Mayer K.F., Moreau H.,
RA Not F., Otillar R., Panaud O., Pangilinan J., Paulsen I., Piegu B.,
RA Poliakov A., Robbens S., Schmutz J., Toulza E., Wyss T., Zelensky A.,
RA Zhou K., Armbrust E.V., Bhattacharya D., Goodenough U.W., Van de Peer Y.,
RA Grigoriev I.V.;
RT "Green evolution and dynamic adaptations revealed by genomes of the marine
RT picoeukaryotes Micromonas.";
RL Science 324:268-272(2009).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
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DR EMBL; CP001333; ACO67665.1; -; Genomic_DNA.
DR RefSeq; XP_002506407.1; XM_002506361.1.
DR AlphaFoldDB; C1EIE0; -.
DR STRING; 296587.C1EIE0; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR GeneID; 8249339; -.
DR KEGG; mis:MICPUN_105010; -.
DR eggNOG; KOG0470; Eukaryota.
DR InParanoid; C1EIE0; -.
DR OMA; HNNSWNH; -.
DR OrthoDB; 610at2759; -.
DR Proteomes; UP000002009; Chromosome 15.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR048650; ISOA1-3-like_C.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF3; ISOAMYLASE 3, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21156; ISOA1-3_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Hydrolase {ECO:0000313|EMBL:ACO67665.1};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000002009};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 261..679
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 575..599
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 788 AA; 87708 MW; B94A5FF0B96036DE CRC64;
MSSRARVGGT NPGAFKPKSS PINRGGANLA RGQPIAAQVE PYSWGDIPTT PSATRDLPSW
LPQPAPVHGD GGPGTFVPPP AFEPVTTPPV IDVDRIRISE GYAQFLGPTP AGDVINPGVN
FALHSAAAAA VDLLIYFDPS STSKQPSLRI PLDPKKNKTG DVWHVRLDNI PRGGDGYVIR
YGYLVDGGKS PHRWDYWEPN QLMVDPYAPL VEGRRVYGKN ETCPNGEVGQ WLGAFALDET
PFDWGGVEPP NIPPQDLVVY ECTPRAFTAS SSSGLDEDVR GSFLGIAEKV QHIKDAGYNA
VELLPVFHFD EMEFKLSPNP RDHMLNTWGY STMAFFAPMT VYASKGAGPQ QAAREFKHMV
KTMHANGIEV LLDVVYNHTG EGSGRFFSFR GIDNKSYYMM EDLNGKVNYK NYTGCGNTFN
CNHEPVMNLV LDSLRHWVDE YHVDGFRFDL TSCLCRDPNS GAIMTSPPVV RAIAKDNTLA
RCKLFAEPWD CAMDGYLVGK FPNWDRWGEW NGIYRDTVRR FLKGDPGLKS QFASSLCGSA
DMYNVNARKP YHSLNFITAH DGFTLRDLVS YNKKQNHQNG EEGRDGCNDN HSWNCGQEGE
SNDQAVASLR WRQMRNMHLA LMVSQGTPMV LMGDEYGHTR KGNNNTYGHD NELNNFDWAA
LEKQRDHYFR YHAGLVKFRK NHPLLGRAEF LNDNDITWHE DNWDNPDSLF LAYQLHDCGQ
GGGDLYIAFN QHDFFVDAAL PPPPGGKSWH RVVDTNLPPP ADFVEHGEGG VGARYNVAPR
GAVMLVAK
//
