ID C1MKB3_MICPC Unreviewed; 467 AA.
AC C1MKB3;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN ORFNames=MICPUCDRAFT_31367 {ECO:0000313|EMBL:EEH59340.1};
OS Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC Mamiellaceae; Micromonas.
OX NCBI_TaxID=564608 {ECO:0000313|Proteomes:UP000001876};
RN [1] {ECO:0000313|EMBL:EEH59340.1, ECO:0000313|Proteomes:UP000001876}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1545 {ECO:0000313|EMBL:EEH59340.1,
RC ECO:0000313|Proteomes:UP000001876};
RX PubMed=19359590; DOI=10.1126/science.1167222;
RA Worden A.Z., Lee J.H., Mock T., Rouze P., Simmons M.P., Aerts A.L.,
RA Allen A.E., Cuvelier M.L., Derelle E., Everett M.V., Foulon E.,
RA Grimwood J., Gundlach H., Henrissat B., Napoli C., McDonald S.M.,
RA Parker M.S., Rombauts S., Salamov A., Von Dassow P., Badger J.H.,
RA Coutinho P.M., Demir E., Dubchak I., Gentemann C., Eikrem W., Gready J.E.,
RA John U., Lanier W., Lindquist E.A., Lucas S., Mayer K.F., Moreau H.,
RA Not F., Otillar R., Panaud O., Pangilinan J., Paulsen I., Piegu B.,
RA Poliakov A., Robbens S., Schmutz J., Toulza E., Wyss T., Zelensky A.,
RA Zhou K., Armbrust E.V., Bhattacharya D., Goodenough U.W., Van de Peer Y.,
RA Grigoriev I.V.;
RT "Green evolution and dynamic adaptations revealed by genomes of the marine
RT picoeukaryotes Micromonas.";
RL Science 324:268-272(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC Evidence={ECO:0000256|ARBA:ARBA00001678};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
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DR EMBL; GG663736; EEH59340.1; -; Genomic_DNA.
DR RefSeq; XP_003055964.1; XM_003055918.1.
DR AlphaFoldDB; C1MKB3; -.
DR STRING; 564608.C1MKB3; -.
DR GeneID; 9681684; -.
DR KEGG; mpp:MICPUCDRAFT_31367; -.
DR eggNOG; ENOG502QQFD; Eukaryota.
DR OrthoDB; 2717493at2759; -.
DR Proteomes; UP000001876; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR001547; Glyco_hydro_5.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR045053; MAN-like.
DR PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR PANTHER; PTHR31451:SF60; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR Pfam; PF00150; Cellulase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW Hydrolase {ECO:0000256|RuleBase:RU361153, ECO:0000313|EMBL:EEH59340.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001876}.
FT DOMAIN 137..409
FT /note="Glycoside hydrolase family 5"
FT /evidence="ECO:0000259|Pfam:PF00150"
SQ SEQUENCE 467 AA; 52801 MW; F4D66ED4D756F6F4 CRC64;
MPSSSLLIMW WSNRSQPSLF VLLPLFMIAV FGSLTTEALD EEYQFSKINL IDVANLSTSV
ALHSAKLRAT KFQLTVQPWE RRQGGEEVRR PRSTENGSES AERFVSVNGS CFALGRRFFP
AIGWNTYTLI EQAAQIPVGS FAFNYSCSGR RQVTDMFDAA VAAGFNTVRT WAYSVGENQS
FQVAPGEYYE PLFQGLDWVI QQASLRDIKL ILVFTDYWEY NGGVAQYLDW AGSRARTKND
FFSNSKCKMM YKNNIQRILE RINTYTGVRY RHDKSIMAWE LINEPRCRNC PEELQDWLEE
MAHFVKFLDR THLLSTGEEG FYSIGSGGSV DANPEVWSLT TGQDFVANHA IPEIDFAVAH
LWPDNWGVFT LGLPASFSET WLVKHKSDSR AILGKPFLLE EFGTTGNGQL SAVDQVGSRF
ATTAERAPRS VAVAEFYRSI FKQVELDNEN EYAIAGDISY SNTPVSR
//
