ID C1MUS7_MICPC Unreviewed; 1079 AA.
AC C1MUS7;
DT 26-MAY-2009, integrated into UniProtKB/TrEMBL.
DT 26-MAY-2009, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE SubName: Full=TWY3 methyltransferase {ECO:0000313|EMBL:EEH56377.1};
GN ORFNames=MICPUCDRAFT_58919 {ECO:0000313|EMBL:EEH56377.1};
OS Micromonas pusilla (strain CCMP1545) (Picoplanktonic green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC Mamiellaceae; Micromonas.
OX NCBI_TaxID=564608 {ECO:0000313|Proteomes:UP000001876};
RN [1] {ECO:0000313|EMBL:EEH56377.1, ECO:0000313|Proteomes:UP000001876}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1545 {ECO:0000313|EMBL:EEH56377.1,
RC ECO:0000313|Proteomes:UP000001876};
RX PubMed=19359590; DOI=10.1126/science.1167222;
RA Worden A.Z., Lee J.H., Mock T., Rouze P., Simmons M.P., Aerts A.L.,
RA Allen A.E., Cuvelier M.L., Derelle E., Everett M.V., Foulon E.,
RA Grimwood J., Gundlach H., Henrissat B., Napoli C., McDonald S.M.,
RA Parker M.S., Rombauts S., Salamov A., Von Dassow P., Badger J.H.,
RA Coutinho P.M., Demir E., Dubchak I., Gentemann C., Eikrem W., Gready J.E.,
RA John U., Lanier W., Lindquist E.A., Lucas S., Mayer K.F., Moreau H.,
RA Not F., Otillar R., Panaud O., Pangilinan J., Paulsen I., Piegu B.,
RA Poliakov A., Robbens S., Schmutz J., Toulza E., Wyss T., Zelensky A.,
RA Zhou K., Armbrust E.V., Bhattacharya D., Goodenough U.W., Van de Peer Y.,
RA Grigoriev I.V.;
RT "Green evolution and dynamic adaptations revealed by genomes of the marine
RT picoeukaryotes Micromonas.";
RL Science 324:268-272(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in
CC tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-
CC carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:36635, Rhea:RHEA-COMP:10378, Rhea:RHEA-
CC COMP:10379, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73543, ChEBI:CHEBI:73550; EC=2.1.1.282;
CC Evidence={ECO:0000256|ARBA:ARBA00001168};
CC -!- SIMILARITY: Belongs to the JARID1 histone demethylase family.
CC {ECO:0000256|ARBA:ARBA00006801}.
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DR EMBL; GG663740; EEH56377.1; -; Genomic_DNA.
DR RefSeq; XP_003059245.1; XM_003059199.1.
DR AlphaFoldDB; C1MUS7; -.
DR STRING; 564608.C1MUS7; -.
DR GeneID; 9684991; -.
DR KEGG; mpp:MICPUCDRAFT_58919; -.
DR eggNOG; KOG1227; Eukaryota.
DR eggNOG; KOG1228; Eukaryota.
DR eggNOG; KOG2132; Eukaryota.
DR OMA; NVFFREL; -.
DR OrthoDB; 122704at2759; -.
DR Proteomes; UP000001876; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 6.10.140.1470; -; 1.
DR Gene3D; 2.60.120.650; Cupin; 1.
DR Gene3D; 3.30.1960.10; tRNA wybutosine-synthesizing-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR041667; Cupin_8.
DR InterPro; IPR003347; JmjC_dom.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR003827; tRNA_yW-synthesising.
DR InterPro; IPR036602; tRNA_yW-synthesising-like_sf.
DR PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23245:SF31; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3 HOMOLOG; 1.
DR Pfam; PF13621; Cupin_8; 1.
DR Pfam; PF02475; Met_10; 1.
DR Pfam; PF02676; TYW3; 1.
DR SMART; SM00558; JmjC; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF111278; SSo0622-like; 1.
DR PROSITE; PS51184; JMJC; 1.
DR PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EEH56377.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001876};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EEH56377.1};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 381..647
FT /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT /evidence="ECO:0000259|PROSITE:PS51684"
FT DOMAIN 824..982
FT /note="JmjC"
FT /evidence="ECO:0000259|PROSITE:PS51184"
SQ SEQUENCE 1079 AA; 114383 MW; C636601591661664 CRC64;
MDAFARRKAD TLAELAIDGG DFSRAGHVDE RARPVVALVN RHPDFFTTSS CAGRVSVFAD
PTTATRAAGM KGGEWVYVNH DPADAADVLA AVRSKLGASD AAGASAPDPE CTLVLRFEPF
ILSVEAKTTR AGAALVAAAR DAGYRESGVT ACDRRTIIAV RCSIRVEVPI VSRGVRLVSE
DALVRLVEIA NEKHALNAAR MERFVVTFAK AFGADLVRAA SAGGGDAGAT AGASEGEDAG
GDATSGAAWV YTVDKNVAKR CKDALKANAW LDKTRKAGGG GGGGGGGGGA RVALPVTAAG
AVALLKLVAE DACDENPAAA AVRDGHAAVL RSNDGAGDVP SLRRKTRQVT PAAAIKSAAT
SLLSARDAAS ASVTSQIPTR WEKLGDLALL PSSSFSDETV WTSDVRTELW PLVAAALGVG
RLAKQAEVSR GPKRESRAVM VYDPKNIGGW VELKENGVIY TLDVTKVMFS SGNGTEKHRM
STQPAAGETV VDLYAGIGYY TLQLLKHAGV SKVYACEWNP NSVAALKKNL VANEIDERRC
EVLEGDNNRT APRGVADRVL LGLLPDSERA WPTAVAALRP ETGGVMHVHG NVASGEEDAW
ARRLEREVAE MAATAGMDWA VRVEHVEKVK WYAPRVRHVV ADVRCAPRRL ATAWAVGVNA
ATSPRSSLTI AGEGKMRARR NPEGAAVAVA AAHSCRAPPL SDGTVRRMHR PTERTFRGEG
GPVRTRVPCV LTGLDLGPAP WLWSPAHLAA KPSVAAADVS VHVCDSPHLD FVRKNFKYEV
MKFGDLLESL SKETTTTGED AKKTWLYLRS IGKNPRKEAA HALEQFPDLK AELKLPSDLL
WGAHDDDAYF STVMRCSSGG TRLWTHYDAM DNALVQLHGE KRVLLFPPSA AAGLYLSGSS
SPVVGPFVDG DGGVWPTEQY PAYERARRRA VEVILQPGDV LFIPALWSHH VESLHGPSVA
MNVFFRELPR AAYPSKDLYG NADPIAAGKA LEMCESAAKE LASLPRDHKV FYGGVAAAKL
LRDLRVETDV MGVTPGGSGH RTRPELLPGK WADAAKGAAL AVAIVGAVSV ATAGWKRAS
//
