ID C2D1B_RAT Reviewed; 850 AA.
AC Q5FVK6;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=Coiled-coil and C2 domain-containing protein 1B;
DE AltName: Full=Five prime repressor element under dual repression-binding protein 2;
DE Short=FRE under dual repression-binding protein 2;
DE Short=Freud-2;
GN Name=Cc2d1b;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, AND MASS
RP SPECTROMETRY.
RC STRAIN=Holtzman; TISSUE=Sperm;
RX PubMed=20966424; DOI=10.2164/jandrol.110.010967;
RA Suryawanshi A.R., Khan S.A., Gajbhiye R.K., Gurav M.Y., Khole V.V.;
RT "Differential proteomics leads to identification of domain specific
RT epididymal sperm proteins.";
RL J. Androl. 32:240-259(2011).
CC -!- FUNCTION: Transcription factor that binds specifically to the DRE (dual
CC repressor element) and represses HTR1A gene transcription in neuronal
CC cells. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CHMP4B. {ECO:0000250|UniProtKB:Q5T0F9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5FVK6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5FVK6-2; Sequence=VSP_025664;
CC -!- TISSUE SPECIFICITY: Expressed in epididymal sperm but not in testicular
CC sperm (at protein level). {ECO:0000269|PubMed:20966424}.
CC -!- MASS SPECTROMETRY: Mass=93476.08; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:20966424};
CC -!- SIMILARITY: Belongs to the CC2D1 family. {ECO:0000305}.
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DR EMBL; AABR03040177; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC089922; AAH89922.1; -; mRNA.
DR RefSeq; NP_001257913.1; NM_001270984.1. [Q5FVK6-1]
DR AlphaFoldDB; Q5FVK6; -.
DR SMR; Q5FVK6; -.
DR STRING; 10116.ENSRNOP00000011670; -.
DR iPTMnet; Q5FVK6; -.
DR PhosphoSitePlus; Q5FVK6; -.
DR jPOST; Q5FVK6; -.
DR PaxDb; 10116-ENSRNOP00000011670; -.
DR Ensembl; ENSRNOT00000011670.8; ENSRNOP00000011670.5; ENSRNOG00000008634.8. [Q5FVK6-1]
DR GeneID; 313478; -.
DR KEGG; rno:313478; -.
DR UCSC; RGD:1306630; rat. [Q5FVK6-1]
DR AGR; RGD:1306630; -.
DR CTD; 200014; -.
DR RGD; 1306630; Cc2d1b.
DR eggNOG; KOG3837; Eukaryota.
DR GeneTree; ENSGT00390000009595; -.
DR HOGENOM; CLU_008808_1_0_1; -.
DR InParanoid; Q5FVK6; -.
DR OMA; NNNCPEY; -.
DR OrthoDB; 2881278at2759; -.
DR PhylomeDB; Q5FVK6; -.
DR TreeFam; TF314229; -.
DR Reactome; R-RNO-9668328; Sealing of the nuclear envelope (NE) by ESCRT-III.
DR PRO; PR:Q5FVK6; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Bgee; ENSRNOG00000008634; Expressed in skeletal muscle tissue and 19 other cell types or tissues.
DR Genevisible; Q5FVK6; RN.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0010008; C:endosome membrane; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd08690; C2_Freud-1; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037772; C2_Freud.
DR InterPro; IPR039725; CC2D1A/B.
DR InterPro; IPR006608; CC2D1A/B_DM14.
DR PANTHER; PTHR13076:SF5; COILED-COIL AND C2 DOMAIN-CONTAINING PROTEIN 1B; 1.
DR PANTHER; PTHR13076; UNCHARACTERIZED; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF21528; CC2D1A-B_DM14; 4.
DR SMART; SM00239; C2; 1.
DR SMART; SM00685; DM14; 4.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR PROSITE; PS50004; C2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..850
FT /note="Coiled-coil and C2 domain-containing protein 1B"
FT /id="PRO_0000288428"
FT DOMAIN 668..807
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 436..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 167..213
FT /evidence="ECO:0000255"
FT COMPBIAS 509..525
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 585
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8BRN9"
FT MOD_RES 588
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8BRN9"
FT VAR_SEQ 1..692
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025664"
SQ SEQUENCE 850 AA; 93534 MW; 2132241D45424146 CRC64;
MPGPRPRKGP KTSGQGAETA KQLGLFVEFN PEDMLLGMDE TEDDGDLEAE LLALTGETGS
TSRKPAPKGR APLPMAHIEK LAADCMRDVE EEGEEEEGLE DDADLLTELQ EVLGVDEETG
LVDDSEETSP DLSEEKTRDN TEQPVAPAAF QQALPAAVAQ AGGPRGLQAL LEERIQNYRE
AAASAKEAGE AAKARRCERG LKTLESQLAT VRKGGKICED EIPPPVALGK RPPAHQERPS
KDSEIDSAGS CAMDPGDLSQ PESSLPAVAA LPDSDPDPQA LLLARQREYK AAALSAKRAG
DLDRARELMR IGKRFGTVLE ALEKGQPVDL SGMPPAPEDL KALPQASEAS AATQVLSPAV
EQMQPVMSSD LPATPAAPAE PKTVLDALQQ RLNRYREAGI QARASGDERK ARMHDRIAKQ
YQDAIRAHQA GRKVDFAELP VPPGFPPIPG LEPRKDTEED SVTATLAAAQ KLASEDTALV
DEDEERDTPA QAPLAKKPAQ PLVPSSHLLN EPKASSSKES LSPSVREQVT LLEARKLQYQ
RAALQAKRRQ DLEQAKSHLR VAKSLEAQII QARAGQPIDL SKVPSPLTDE EGDFILIHHE
DLRLSQKAEE VYAQLQKILL EQHEKCLLFS KQFMHQGNVA ETTRFEKLAE DRKKQLEILQ
LAQAQGLDPP SHHFELKTLQ TVRIFSELNS TEMHLIIVRG MNLPAPPGVT PDDLDAFVRF
EFHYPNSDQA QKSKTAVVKN TNSPEFEQVF KLNINRNHRG FRRVIQSKGI KFEIFHKGSF
FRSDKLVGTA HLKLERLEKE CEIREIMEVL DGRKPTGGKL EVKVRLREPL SSQDVQMVTE
NWLVLEPRGL
//
