ID C3Y6N2_BRAFL Unreviewed; 749 AA.
AC C3Y6N2;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=BRAFLDRAFT_213523 {ECO:0000313|EMBL:EEN63992.1};
OS Branchiostoma floridae (Florida lancelet) (Amphioxus).
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomatidae; Branchiostoma.
OX NCBI_TaxID=7739;
RN [1] {ECO:0000313|EMBL:EEN63992.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 {ECO:0000313|EMBL:EEN63992.1};
RC TISSUE=Testes {ECO:0000313|EMBL:EEN63992.1};
RX PubMed=18563158; DOI=10.1038/nature06967;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Putnam N.H., Butts T., Ferrier D.E.K., Furlong R.F., Hellsten U.,
RA Kawashima T., Robinson-Rechavi M., Shoguchi E., Terry A., Yu J.-K.,
RA Benito-Gutierrez E.L., Dubchak I., Garcia-Fernandez J., Gibson-Brown J.J.,
RA Grigoriev I.V., Horton A.C., de Jong P.J., Jurka J., Kapitonov V.V.,
RA Kohara Y., Kuroki Y., Lindquist E., Lucas S., Osoegawa K., Pennacchio L.A.,
RA Salamov A.A., Satou Y., Sauka-Spengler T., Schmutz J., Shin-I T.,
RA Toyoda A., Bronner-Fraser M., Fujiyama A., Holland L.Z., Holland P.W.H.,
RA Satoh N., Rokhsar D.S.;
RT "The amphioxus genome and the evolution of the chordate karyotype.";
RL Nature 453:1064-1071(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; GG666488; EEN63992.1; -; Genomic_DNA.
DR RefSeq; XP_002607982.1; XM_002607936.1.
DR AlphaFoldDB; C3Y6N2; -.
DR eggNOG; KOG2177; Eukaryota.
DR InParanoid; C3Y6N2; -.
DR GO; GO:0030371; F:translation repressor activity; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0017148; P:negative regulation of translation; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR CDD; cd05819; NHL; 2.
DR Gene3D; 3.30.160.60; Classic Zinc Finger; 1.
DR Gene3D; 2.120.10.30; TolB, C-terminal domain; 2.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR001258; NHL_repeat.
DR InterPro; IPR047153; TRIM45/56/19.
DR InterPro; IPR000315; Znf_B-box.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR25462; BONUS, ISOFORM C-RELATED; 1.
DR PANTHER; PTHR25462:SF229; NHL (RING FINGER B-BOX COILED COIL) DOMAIN CONTAINING; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR Pfam; PF00097; zf-C3HC4; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57845; B-box zinc-binding domain; 1.
DR SUPFAM; SSF101898; NHL repeat; 2.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51125; NHL; 2.
DR PROSITE; PS50119; ZF_BBOX; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00024}.
FT DOMAIN 16..58
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 109..149
FT /note="B box-type"
FT /evidence="ECO:0000259|PROSITE:PS50119"
FT REPEAT 526..569
FT /note="NHL"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00504"
FT REPEAT 665..708
FT /note="NHL"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00504"
FT COILED 178..238
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 749 AA; 82374 MW; 8626FB3D24621F0F CRC64;
MASNAISDIT DEFLVCQVCL EDFKQPKMLP CLHTFCQPCL EKLLAIEPVG KLDCPTCRQG
AVPLPENGVQ GLKSNFLVGK LRDILQQQPQ QQPKGESSQA REEGVLCAVE TSKCEDHHEL
NKFYCDTCHR VICLHCVVTA HKDHRYVEIE KAAEREGAKI KAKLATVKNT ADLHQDWIRD
LQSVKDEWST QVQRTEEQIR KQVNSIIQAA KKAGNDRIAQ LRAMNAAQEK QLQAAMEAAE
MDLASARSCV QFTDNVLEYG SPAELMSVAG ELTGRLDQTA DDKTAGKAEM TKDLVNLIVK
PSRCNVEQEV SKLVGEITKS LIPVLSSPLT LTTQVRHNIV IGKDGGGLKR IKVVGENGFG
QFIGLESLAV TAEGDIVATD RGNSRLQFWG KDGSFKKKVD LRFKPLCVAA LTNGDLLVTG
DGHKIHVLDK QGRESRAIQV TEAEEKDEPT LGITVDGLGR IIVTIGHQVF KLGPGGDVMM
RIGGKDQGQQ YFGSSLRVAV NSRNQIIVSD ECNSNMMMFD PSGRRLFTCG SRGSGPGQLD
PPHCVITDSE DDIIVSDYNN HRVSLFSRDG TFIRHVLTKT EHGLNGPMGL ALTHDGHLIV
SDNLRCCLFK LSPSGDVMMG IEGEDQGQQY FGSSLCVAVN SRNQIIVSDW HNIKMMMFDP
SGRHLFTCGS RVSGPGQLNT PHCVITDSED DIIVSDWNNR RVSLFSQDGT FIRHVLTKTE
HGLNGPMGLA LTLDGHLIVS EYNNITFFM
//
