ID C4LAT6_TOLAT Unreviewed; 297 AA.
AC C4LAT6;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 67.
DE RecName: Full=Nitrogen fixation protein NifU {ECO:0000256|ARBA:ARBA00015278, ECO:0000256|PIRNR:PIRNR000375};
GN OrderedLocusNames=Tola_0661 {ECO:0000313|EMBL:ACQ92290.1};
OS Tolumonas auensis (strain DSM 9187 / TA4).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Tolumonas.
OX NCBI_TaxID=595494 {ECO:0000313|EMBL:ACQ92290.1, ECO:0000313|Proteomes:UP000009073};
RN [1] {ECO:0000313|Proteomes:UP000009073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 9187 / TA4 {ECO:0000313|Proteomes:UP000009073};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Spring S.,
RA Beller H.;
RT "Complete sequence of Tolumonas auensis DSM 9187.";
RL Submitted (MAY-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACQ92290.1, ECO:0000313|Proteomes:UP000009073}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 9187 / TA4 {ECO:0000313|Proteomes:UP000009073};
RX PubMed=22180815; DOI=10.4056/sigs.2184986;
RA Chertkov O., Copeland A., Lucas S., Lapidus A., Berry K.W., Detter J.C.,
RA Del Rio T.G., Hammon N., Dalin E., Tice H., Pitluck S., Richardson P.,
RA Bruce D., Goodwin L., Han C., Tapia R., Saunders E., Schmutz J.,
RA Brettin T., Larimer F., Land M., Hauser L., Spring S., Rohde M.,
RA Kyrpides N.C., Ivanova N., Goker M., Beller H.R., Klenk H.P., Woyke T.;
RT "Complete genome sequence of Tolumonas auensis type strain (TA 4).";
RL Stand. Genomic Sci. 5:112-120(2011).
CC -!- FUNCTION: May be involved in the formation or repair of [Fe-S] clusters
CC present in iron-sulfur proteins. {ECO:0000256|PIRNR:PIRNR000375}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000256|ARBA:ARBA00006420,
CC ECO:0000256|PIRNR:PIRNR000375}.
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DR EMBL; CP001616; ACQ92290.1; -; Genomic_DNA.
DR RefSeq; WP_012728889.1; NC_012691.1.
DR AlphaFoldDB; C4LAT6; -.
DR STRING; 595494.Tola_0661; -.
DR KEGG; tau:Tola_0661; -.
DR eggNOG; COG0694; Bacteria.
DR eggNOG; COG0822; Bacteria.
DR HOGENOM; CLU_079283_0_0_6; -.
DR OrthoDB; 9808097at2; -.
DR Proteomes; UP000009073; Chromosome.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd06664; IscU_like; 1.
DR CDD; cd19947; NifU_Fer2_BFD-like; 1.
DR Gene3D; 3.90.1010.10; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR016217; N_fixation_NifU.
DR InterPro; IPR010238; NIF_FeS_clus_asmbl_NifU.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR NCBIfam; TIGR02000; NifU_proper; 1.
DR PANTHER; PTHR10093:SF8; IRON-SULFUR CLUSTER ASSEMBLY ENZYME ISCU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR10093; IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01106; NifU; 1.
DR Pfam; PF01592; NifU_N; 1.
DR PIRSF; PIRSF000375; NifU; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF82649; SufE/NifU; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR000375-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000375-1};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|PIRNR:PIRNR000375};
KW Reference proteome {ECO:0000313|Proteomes:UP000009073}.
FT DOMAIN 4..124
FT /note="NIF system FeS cluster assembly NifU N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01592"
FT DOMAIN 135..184
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 223..287
FT /note="NIF system FeS cluster assembly NifU C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01106"
FT BINDING 35
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 62
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 106
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 137
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 139
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 172
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 175
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
SQ SEQUENCE 297 AA; 32136 MW; 4DE49B95001E920A CRC64;
MWNYSEKVKD HFFNPRNAKV IADANAVGDV GSISCGDALR LMLQVNAESE IIEDAGFQTF
GCGSAIASSS ALTEMVIGKT LDDALKISNM DIADYLDGLP PEKMHCSVMG QEALQAAIAN
FRGEEWHDDH EEGALICKCF AVDEVKIIKA VKENGLTSIA DVINYTKAGG GCGACHEKIE
QALQKVLATQ PCNEARIATT SMERSKVAPH VDLAKKDENW RTVAAVLEEM RPRLQADGGD
VTLISVTESK VEVALSGSCL GCMMTDMTIA WIQQTLMEKM GRYIQVVSIT QAETALV
//
