ID   C4QWT9_KOMPG            Unreviewed;       249 AA.
AC   C4QWT9;
DT   07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT   07-JUL-2009, sequence version 1.
DT   24-JAN-2024, entry version 65.
DE   SubName: Full=Member of the alpha/beta knot fold methyltransferase superfamily {ECO:0000313|EMBL:CAY67712.1};
GN   OrderedLocusNames=PAS_chr1-1_0335 {ECO:0000313|EMBL:CAY67712.1};
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223 {ECO:0000313|EMBL:CAY67712.1, ECO:0000313|Proteomes:UP000000314};
RN   [1] {ECO:0000313|EMBL:CAY67712.1, ECO:0000313|Proteomes:UP000000314}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864 {ECO:0000313|Proteomes:UP000000314};
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase NEP1 family.
CC       {ECO:0000256|ARBA:ARBA00008115}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FN392319; CAY67712.1; -; Genomic_DNA.
DR   RefSeq; XP_002489993.1; XM_002489948.1.
DR   AlphaFoldDB; C4QWT9; -.
DR   SMR; C4QWT9; -.
DR   STRING; 644223.C4QWT9; -.
DR   EnsemblFungi; CAY67712; CAY67712; PAS_chr1-1_0335.
DR   GeneID; 8196600; -.
DR   KEGG; ppa:PAS_chr1-1_0335; -.
DR   eggNOG; KOG3073; Eukaryota.
DR   HOGENOM; CLU_055846_1_1_1; -.
DR   InParanoid; C4QWT9; -.
DR   OMA; VHNTFEL; -.
DR   OrthoDB; 275493at2759; -.
DR   Proteomes; UP000000314; Chromosome 1.
DR   GO; GO:0030686; C:90S preribosome; IEA:EnsemblFungi.
DR   GO; GO:0005737; C:cytoplasm; IEA:EnsemblFungi.
DR   GO; GO:0005880; C:nuclear microtubule; IEA:EnsemblFungi.
DR   GO; GO:0034399; C:nuclear periphery; IEA:EnsemblFungi.
DR   GO; GO:0005730; C:nucleolus; IEA:EnsemblFungi.
DR   GO; GO:0032040; C:small-subunit processome; IEA:EnsemblFungi.
DR   GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR   GO; GO:0070037; F:rRNA (pseudouridine) methyltransferase activity; IEA:EnsemblFungi.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000480; P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000447; P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0000472; P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IEA:EnsemblFungi.
DR   GO; GO:0070475; P:rRNA base methylation; IEA:EnsemblFungi.
DR   CDD; cd18088; Nep1-like; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR005304; Rbsml_bgen_MeTrfase_EMG1/NEP1.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   PANTHER; PTHR12636; NEP1/MRA1; 1.
DR   PANTHER; PTHR12636:SF5; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1; 1.
DR   Pfam; PF03587; EMG1; 1.
DR   SUPFAM; SSF75217; alpha/beta knot; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:CAY67712.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000314};
KW   Ribosome biogenesis {ECO:0000256|ARBA:ARBA00022517};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW   rRNA processing {ECO:0000256|ARBA:ARBA00022552};
KW   rRNA-binding {ECO:0000256|ARBA:ARBA00022730};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
SQ   SEQUENCE   249 AA;  27838 MW;  DBEBC0A0B20FFEB2 CRC64;
     MVEDSKQRDT RTKVEIPEAS LVPQRPTVLT SKDKTTQRLI VVLSQASLET HRMTSSGHND
     KYALLNCDDH QALLRKMSRD ISEARPDITH QCLLTLLDSP INKAGKLQVY IQTAKGVLIE
     VNPTVRIPRT FKRFSGLMVQ LLHKLSIRSV NSEEKLLKVI KNPITDHLPT KCRKITLSFD
     AEVVKVSSYI EKLASDESIC VFVGAMARGK DDFADEFVDE KIGVSEYPLS ASVACSKFCH
     GAEDAWSIL
//