ID C4QXN6_KOMPG Unreviewed; 701 AA.
AC C4QXN6;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN OrderedLocusNames=PAS_chr1-4_0175 {ECO:0000313|EMBL:CAY68009.1};
OS Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=644223 {ECO:0000313|EMBL:CAY68009.1, ECO:0000313|Proteomes:UP000000314};
RN [1] {ECO:0000313|EMBL:CAY68009.1, ECO:0000313|Proteomes:UP000000314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS115 / ATCC 20864 {ECO:0000313|Proteomes:UP000000314};
RX PubMed=19465926; DOI=10.1038/nbt.1544;
RA De Schutter K., Lin Y.C., Tiels P., Van Hecke A., Glinka S.,
RA Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT "Genome sequence of the recombinant protein production host Pichia
RT pastoris.";
RL Nat. Biotechnol. 27:561-566(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; FN392319; CAY68009.1; -; Genomic_DNA.
DR RefSeq; XP_002490290.1; XM_002490245.1.
DR AlphaFoldDB; C4QXN6; -.
DR SMR; C4QXN6; -.
DR STRING; 644223.C4QXN6; -.
DR EnsemblFungi; CAY68009; CAY68009; PAS_chr1-4_0175.
DR GeneID; 8197869; -.
DR KEGG; ppa:PAS_chr1-4_0175; -.
DR eggNOG; KOG2108; Eukaryota.
DR HOGENOM; CLU_004585_7_0_1; -.
DR InParanoid; C4QXN6; -.
DR OMA; ENGWRGL; -.
DR OrthoDB; 1981871at2759; -.
DR Proteomes; UP000000314; Chromosome 1.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF46; ATP-DEPENDENT DNA HELICASE HMI1, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000000314}.
FT DOMAIN 15..277
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 277..549
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 701 AA; 79359 MW; 72E683FB35F55399 CRC64;
MENEDSTDSL ELKITDDQFR SITYSHEPLT ALKIVAGPGC GKTHTLIARI VHLLNSEVDP
CQILVLSLTN RAVLSLRSRL IDLVGTELAQ QVNIQTFHSF AMNVLNDLIP NQYELLGFGG
TQMISKLVNV PFLKRKDQIT KLLEQQMINR MSSSQKTSST SSSNLKVQQL VKFLHNTNVL
TSSSIISQCT LLLRENSQKL NHRVLIVDEF QDMYPELVQL VAVLASDCHL TVAGDPDQSI
YSFLGSTPYV LNGLDKILPH NYSKIETINL TATFRMSLPI IESSQLFSMF KKPLTPVESP
APNYLPKPTK KYFTDIAQEV DFILEEISRL VHQCGGVLKP SDFAILSFKN DDINYAQSVF
EKQGIKTQKL GSSPEWTNNE LYHFINFLRV INDPGADYPL ISCLSIIPGV GHVSLSSLVL
KAMSSGSPIF HLLKGVRPVI DSFVEDLNTV RDRFFQYFDD PDEIFKITLE FASKYGLLQA
IKSNPNWSQK PDSFYFKQLE SIRVGFHMAS KLRSPNETLV QYFIAHFADE IVQPTEDAIN
FSTIHGAKGL EFPVVFLLGA TQVSTFRSEE TKRLIYVALT RAKQLLYWNK YLYHEDASEE
YKFQNVFTDS IPSCSYEYVK YLSKITRRTP PSLNDYHQGI MTWKTTKKKP QLLLETPSKS
KGQNGETHPI LYPNSVALPF MKLGTRCYHS AVSGLLRRVI K
//
