ID C4QZ19_KOMPG Unreviewed; 981 AA.
AC C4QZ19;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE SubName: Full=C-1-tetrahydrofolate synthase, mitochondrial {ECO:0000313|EMBL:CAY68493.1};
GN OrderedLocusNames=PAS_chr1-4_0632 {ECO:0000313|EMBL:CAY68493.1};
OS Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=644223 {ECO:0000313|EMBL:CAY68493.1, ECO:0000313|Proteomes:UP000000314};
RN [1] {ECO:0000313|EMBL:CAY68493.1, ECO:0000313|Proteomes:UP000000314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS115 / ATCC 20864 {ECO:0000313|Proteomes:UP000000314};
RX PubMed=19465926; DOI=10.1038/nbt.1544;
RA De Schutter K., Lin Y.C., Tiels P., Van Hecke A., Glinka S.,
RA Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT "Genome sequence of the recombinant protein production host Pichia
RT pastoris.";
RL Nat. Biotechnol. 27:561-566(2009).
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the
CC formate--tetrahydrofolate ligase family.
CC {ECO:0000256|ARBA:ARBA00006985}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate
CC dehydrogenase/cyclohydrolase family. {ECO:0000256|ARBA:ARBA00005559}.
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DR EMBL; FN392319; CAY68493.1; -; Genomic_DNA.
DR RefSeq; XP_002490773.1; XM_002490728.1.
DR AlphaFoldDB; C4QZ19; -.
DR SMR; C4QZ19; -.
DR STRING; 644223.C4QZ19; -.
DR EnsemblFungi; CAY68493; CAY68493; PAS_chr1-4_0632.
DR GeneID; 8197840; -.
DR KEGG; ppa:PAS_chr1-4_0632; -.
DR eggNOG; KOG4230; Eukaryota.
DR HOGENOM; CLU_003601_2_0_1; -.
DR InParanoid; C4QZ19; -.
DR OMA; IKRVVDC; -.
DR OrthoDB; 651667at2759; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000000314; Chromosome 1.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR CDD; cd01080; NAD_bind_m-THF_DH_Cyclohyd; 1.
DR Gene3D; 1.10.8.770; -; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_01543; FTHFS; 1.
DR HAMAP; MF_01576; THF_DHG_CYH; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000672; THF_DH/CycHdrlase.
DR InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom.
DR InterPro; IPR020867; THF_DH/CycHdrlase_CS.
DR InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR PANTHER; PTHR48099:SF5; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR48099; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED; 1.
DR Pfam; PF01268; FTHFS; 1.
DR Pfam; PF00763; THF_DHG_CYH; 1.
DR Pfam; PF02882; THF_DHG_CYH_C; 1.
DR PRINTS; PR00085; THFDHDRGNASE.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00767; THF_DHG_CYH_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000000314}.
FT DOMAIN 41..157
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT catalytic"
FT /evidence="ECO:0000259|Pfam:PF00763"
FT DOMAIN 162..326
FT /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF02882"
SQ SEQUENCE 981 AA; 104657 MW; B0133252A008955F CRC64;
MLSRIGSRNK AASTVRRLAG DLVLRSRQLH ASAVFSQAQI LSGTKLAKSI RSKVFEEINV
FKATHPEFQP AVTIIQVGNR PDSSAYVRMK LKAASESGII CNVIKLPEDT AEPTLVNRIH
KLNNDSSVHG ILVQLPLPSH IDETHITNSV SILKDVDGFD RFNVGELAKK GGNPTFLPCT
PNGCMKLLEA SGVELSGKSA VVLGRSDIVG TPVAQLLNKA NCTVTICHSR TPNIADIVKN
SDIVVAAIGK ANFVKGEWLK PGAVVIDVGI NYVPDSTKKS GQRLVGDVEF ESAKEKASVI
TPVPGGVGPM TVAMLVSNIA HAAKLQLKND LKPAKIYANP LELKTPVPSD IEISRAQEPR
YIKDIATDLG IKDKELELYG HYKAKVSLDV YDRLAENREN GNYVLVAGIT PTPLGEGKST
TTMGLVQALG AHLNLAAVAN VRQPSMGPTF GVKGGAAGGG YAQVIPMDEF NMHLTGDIHA
IGAANNLLAA ALDTRIFHET TQKDAVFYKR LVPAKKGVRK FTPSMLKRLE KLGINKTDPD
SLTPEEATKF AKLNIDPQTI TVKRVLDVND RFVRQVTIGE APTEKGHTRK TGFDITVASE
VMAILALSKD LKDLRSRVGA IVVASSYEGV PITAEDLGVA GALTALLKDA VKPNLMQTLE
GTPVFVHAGP FANISIGASS VIADRVALKL IGASKSDVVA KSEKGFVVTE AGFDFTMGGE
RFYNIKCRAS GLKPNTVVLV ATSRALKLHG GAPDVKPGQA LPEEYTTENV ELVRRGCANL
AKQISNASQY GAPVVVAINQ FETDSPAELA VIREEALKAG AFDAVPSNHW AEGGKGAVEL
AKAVVSSCAS CPEPNAEQSE LYSLDNNTIE DRLNIIVRKM YNGNGIELSE LAKTQIARYE
AQGFGNLPIC IAKTQYSLSH DPSLKNVPSG FTVPIREVRL SAGAGYLSCL AAAIMTIPGL
PTHPGYLNVE VNDEGEIEGL F
//
