ID C4R8E5_KOMPG Unreviewed; 327 AA.
AC C4R8E5;
DT 07-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 07-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE SubName: Full=Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-l {ECO:0000313|EMBL:CAY71870.1};
GN OrderedLocusNames=PAS_chr4_0610 {ECO:0000313|EMBL:CAY71870.1};
OS Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=644223 {ECO:0000313|EMBL:CAY71870.1, ECO:0000313|Proteomes:UP000000314};
RN [1] {ECO:0000313|EMBL:CAY71870.1, ECO:0000313|Proteomes:UP000000314}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS115 / ATCC 20864 {ECO:0000313|Proteomes:UP000000314};
RX PubMed=19465926; DOI=10.1038/nbt.1544;
RA De Schutter K., Lin Y.C., Tiels P., Van Hecke A., Glinka S.,
RA Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT "Genome sequence of the recombinant protein production host Pichia
RT pastoris.";
RL Nat. Biotechnol. 27:561-566(2009).
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000256|ARBA:ARBA00002791}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; FN392322; CAY71870.1; -; Genomic_DNA.
DR RefSeq; XP_002494049.1; XM_002494004.1.
DR AlphaFoldDB; C4R8E5; -.
DR SMR; C4R8E5; -.
DR STRING; 644223.C4R8E5; -.
DR EnsemblFungi; CAY71870; CAY71870; PAS_chr4_0610.
DR GeneID; 8200875; -.
DR KEGG; ppa:PAS_chr4_0610; -.
DR eggNOG; KOG2603; Eukaryota.
DR HOGENOM; CLU_052855_1_0_1; -.
DR InParanoid; C4R8E5; -.
DR OMA; VLFGMYS; -.
DR OrthoDB; 4604288at2759; -.
DR Proteomes; UP000000314; Chromosome 4.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR InterPro; IPR021149; OligosaccharylTrfase_OST3/OST6.
DR PANTHER; PTHR12692; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR12692:SF0; GH11935P; 1.
DR Pfam; PF04756; OST3_OST6; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000000314};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..327
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5009950967"
FT TRANSMEM 179..200
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 212..230
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 262..280
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 292..314
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 327 AA; 36999 MW; AB1583079AA2336C CRC64;
MKLLHSIFWL TAIIQLSLAL TYKQLQENYL KGANILKIDN KNYKLIEEAR EDFELVLFIT
ANGADVNCRL CKEFQTPFEI LANSYATNKA HYSEEEIGKP VVFAISDFTY SRKFFEALKL
QNVPKIFHYA STEKASSALN VDDEYDFTAA DLITHLRNWI TAKTKVDPKL LEIHIPPNYN
AIATNFMVAL IILIVIYKKY ELILTFLGNK NLWQGLSMVT IVAFIAGHMF NTIRNTQYIM
VTQGGSPVYI QPGHQQQLGV ETQIVGLIYG SLFALSVLLY KAGASKEGSS GWLSILISVL
VFLLYSGLIG VFHLKNMGYP YSLIHLF
//
