ID C5CE93_KOSOT Unreviewed; 260 AA.
AC C5CE93;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=CRISPR-associated endoribonuclease {ECO:0000256|PIRNR:PIRNR005054};
GN OrderedLocusNames=Kole_0488 {ECO:0000313|EMBL:ACR79211.1};
OS Kosmotoga olearia (strain ATCC BAA-1733 / DSM 21960 / TBF 19.5.1).
OC Bacteria; Thermotogota; Thermotogae; Kosmotogales; Kosmotogaceae;
OC Kosmotoga.
OX NCBI_TaxID=521045 {ECO:0000313|EMBL:ACR79211.1, ECO:0000313|Proteomes:UP000002382};
RN [1] {ECO:0000313|EMBL:ACR79211.1, ECO:0000313|Proteomes:UP000002382}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1733 / DSM 21960 / TBF 19.5.1
RC {ECO:0000313|Proteomes:UP000002382};
RG US DOE Joint Genome Institute;
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C.,
RA Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA Noll K.;
RT "Complete sequence of Thermotogales bacterium TBF 19.5.1.";
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ACR79211.1, ECO:0000313|Proteomes:UP000002382}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1733 / DSM 21960 / TBF 19.5.1
RC {ECO:0000313|Proteomes:UP000002382};
RX PubMed=21914881; DOI=10.1128/JB.05828-11;
RA Swithers K.S., Dipippo J.L., Bruce D.C., Detter C., Tapia R., Han S.,
RA Goodwin L.A., Han J., Woyke T., Pitluck S., Pennacchio L., Nolan M.,
RA Mikhailova N., Land M.L., Nesbo C.L., Gogarten J.P., Noll K.M.;
RT "Genome Sequence of Kosmotoga olearia Strain TBF 19.5.1, a Thermophilic
RT Bacterium with a Wide Growth Temperature Range, Isolated from the Troll B
RT Oil Platform in the North Sea.";
RL J. Bacteriol. 193:5566-5567(2011).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC {ECO:0000256|PIRNR:PIRNR005054}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000256|ARBA:ARBA00005937, ECO:0000256|PIRNR:PIRNR005054}.
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DR EMBL; CP001634; ACR79211.1; -; Genomic_DNA.
DR RefSeq; WP_012744998.1; NC_012785.1.
DR AlphaFoldDB; C5CE93; -.
DR STRING; 521045.Kole_0488; -.
DR KEGG; kol:Kole_0488; -.
DR eggNOG; COG1583; Bacteria.
DR HOGENOM; CLU_089858_2_0_0; -.
DR OrthoDB; 9797488at2; -.
DR Proteomes; UP000002382; Chromosome.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd21140; Cas6_I-like; 1.
DR Gene3D; 3.30.70.1890; -; 1.
DR Gene3D; 3.30.70.1900; -; 1.
DR InterPro; IPR049435; Cas_Cas6_C.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR NCBIfam; TIGR01877; cas_cas6; 1.
DR PANTHER; PTHR36984; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR PANTHER; PTHR36984:SF1; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR Pfam; PF21350; Cas6_I-A; 1.
DR Pfam; PF01881; Cas_Cas6_C; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Reference proteome {ECO:0000313|Proteomes:UP000002382};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 128..255
FT /note="CRISPR associated protein Cas6 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01881"
FT ACT_SITE 28
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT ACT_SITE 43
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT SITE 55
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-1"
SQ SEQUENCE 260 AA; 30023 MW; 84CE68F0771FDA38 CRC64;
MRIKVILKEA KNKAFKRDHY HQLAGMIYNM IRKSNPEFSS WLHEKGFMDG NKKFKFFCFS
RLIAPRNHYE YIGENKELIL FKTDQVSLYI SSPVEEFLTN LIEFFLAEDY VKLGDHTLKV
DSARALTTPD FGDKTVFKAI TPIVLSKRVE GYNTPFYIRA YQSPDEFDEY LTKNAKEKWK
IFTGQENADI SLQVDRTYLE KKGKRTSCKI NLSPNQTIIG SVVPVIAIGD SEIIRFLYDV
GIGQKNSLGM GMVELAGYKY
//
