ID C5DM02_LACTC Unreviewed; 3254 AA.
AC C5DM02;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 24-JAN-2024, entry version 76.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN OrderedLocusNames=KLTH0G04884g {ECO:0000313|EMBL:CAR24813.1};
OS Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
OS (Yeast) (Kluyveromyces thermotolerans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Lachancea.
OX NCBI_TaxID=559295 {ECO:0000313|EMBL:CAR24813.1, ECO:0000313|Proteomes:UP000002036};
RN [1] {ECO:0000313|EMBL:CAR24813.1, ECO:0000313|Proteomes:UP000002036}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 56472 / CBS 6340 / NRRL Y-8284
RC {ECO:0000313|Proteomes:UP000002036};
RX PubMed=19525356; DOI=10.1101/gr.091546.109;
RG The Genolevures Consortium;
RA Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C.,
RA Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V.,
RA Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R., Durrens P.,
RA Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L.,
RA Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V.,
RA Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I.,
RA Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A.,
RA Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L.,
RA Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.;
RT "Comparative genomics of protoploid Saccharomycetaceae.";
RL Genome Res. 19:1696-1709(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; CU928171; CAR24813.1; -; Genomic_DNA.
DR RefSeq; XP_002555250.1; XM_002555204.1.
DR STRING; 559295.C5DM02; -.
DR GeneID; 8293516; -.
DR KEGG; lth:KLTH0G04884g; -.
DR eggNOG; KOG0939; Eukaryota.
DR HOGENOM; CLU_000215_0_1_1; -.
DR InParanoid; C5DM02; -.
DR OMA; DCHFSRE; -.
DR OrthoDB; 164548at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000002036; Chromosome G.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 2.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000002036};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 2918..3254
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1881..1903
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1947..2016
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2050..2103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2288..2319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2412..2438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2056..2075
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2078..2094
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2301..2317
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3221
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3254 AA; 370631 MW; 6CF69CABFD26F78B CRC64;
MVTLTKLEKF RKEEKAKGFK PLLDDLTQCS EAEFVKKLTN IITWDRSRDD LYIWIPVLNR
IDDILNRLLA HYNYKSTNRK TQPVKLIIMS AEDETVTKEL LWFTCRLLNN TCNRSLYSSL
DVMTNLLNCP NYVVKLGAMK VAAIVGEQQV IARQKIDSKN LLSNKELKRK TLDLALALPS
STTDDRMDHF ALVDLFFDKK QYPTKWSKID FSYYAAAPKV YLTQGALNKT GTGSVKRFIM
NAEELSSLSL QQVFDKGMAE IPAESWFDFS LQATVAKAFS DSTYENLQLR NLIIQTKFNA
IALANVIYIP AQVSSQLFEV DPYAFNNLTD FISLSEQKLP KDLRVDALFS LECISLKHIW
CSDIVRNLGG NMSHGLLFQI LRYIAKLLRD NNSKELDEEY NVRFFYLVSN LADVKTLQES
MLAAGLIPNL LEIISIKDCA YKRTLASATH LLEVFIDDAD ATTEFIGNNG FNILIGCVNE
EVNFALEHPE FGEPPKYPVV YYSISFRQLG YIRSLLKLVL KLLKTDSGDR IRNLIDSPIL
LSLNQILENR PVFGYTLVTY ALDVVQTILN TEPTIYSVLV ESKTIPYIID NFKHFLGPSG
ELLCLLPEVI SALSLNNDGL KQVKEKGLIN CLFEIMTTPE LAKIMSWDDS AIDLGTSIDE
LARHYPELKP VIEDCFYKTV KALPSIISFS HPYVYRSTIS NGEFYHSRDE TVVDNEEGGS
EIESGEVQVP AAVLDCFSAV FYGMTLENVS WASLAEKVDI KDLLKVIIIE RPPYDYVGSQ
TLLNFSDILK HFDDERRNYA LPALLSLLSE KLDGVKDFLE YNFEKPYVLS TPYEVLDETL
RKLTVINVLL YVITDIYINI ASLFPIRVLQ IMEFFEQNGF NLIVKLRHLF QRSALEELYM
RNRLPASVTA ETTMRPIGNT PPLTIYASKP SKNDPKDDKT SAKFKNTLQD RQLFIKMQSW
TSMLFRCFLR LTHARKMTVE ASDRALEIRI FDTTVNEIVK MMDLKYLLSH LGYFLVVFNF
NTFVFTFPNV TTSVGGTVQT IPALLFYQAG GFKIYYEAIK LLFANLQKFN DVENIEKFDY
IKDSEDILTA SALINALSFF NKCIQMETME NIRNTKEYYP YDEVYYNLTS ALIIPIKVLS
LGLVDELVNE FSILEQGTRR LPYSVFKQLL TMIKNIFNST AEGEDHDLYE LRWDLMPPSH
RKIEMLKSCG IEEDIAKNYL EEQKDDLPIH VKPDVFSDGE WETYKALRET GKWKTDLSLL
EPQYKNFSTA SDLSEMRSSF YANGFEDRIL QLLPMYPKLI NAISHMILQL LQEAEEPPKK
VLGKLLQMMR GIDLSDASVL APVIHLFGIL LNDKAMYEKS EDFIHEFVQY LQQSLRPEFV
NSAWFSKALY VYEIIFAKSE APRLDPLPDG IEEGVNTPCP PLFKAFTISS DAKSKIFDSL
IRVHEISDFY SALAISRILI LYTRDEHYSH EVPQSGILSK ILKTIGIFQK SEKINYLESS
FLLLARRCFE TSEIVSSLIQ YELSRAFTTR AIGDSKENSR DLTGLVSEKA NVVMRDPASF
VNCLCETARF EDFVRPDKLE NLIVRRYLSE RDEEMSDANS QADGKLSLSN RTGIVHLLLS
QLMAAYKKDW TSEPVKEESV EDTKKKDDVD VARNPVCAYM IFLLKVLIEL VGSYKQSKLE
FLTFNKRNMY VESPKPRTTA LNFVLYQLLD TGGRDQDKHY VERKEVIGKL ARDLVVSFVS
SVQDKEVQKQ DPKIVNPDMT YIRKFTIEAV TKALKSVAKS PKTLEANVSK LYGWLHLVTS
FLVSERSYLH SVLDSNKTYV DKYQIGKLML DMNVPSTIAD CMASLDLNYP FSKKLFNKSV
EPLNAINEIR SRFSDLFKLE NTDEDDEVED ESEKEEHSDM FRNSALGMYD VEDIEEDDDD
DASLIGEDED IAFVEGEDGE IEVVFSEDER GHHHGGSSSS DGMNDSDQID DSEGSMDYGL
DGESLDMDIA SGESSNSDIG NGGDGIESDD SSYYTDHEGP LEYVHIDDGA PAPVIDVELD
SSELAESDWE SRLSDLSGSD WESGLSELSG SEDGSSEENF NDETHPGSRW LADEVDMDDD
SDEEGRGVFT GIQHVFSPEP QYFRVHGENH RGHRGPHHHS SRRADFMMAT PMSLSTGSRG
TRGILMNPLG PSGLEEVENG ILNQLTSTGS SSRPRNELPH LSDILFSGEF FDEKSSVGIV
LKSTTARWSD IFDMFYDSKI YANNVIPTII GRIFQPSSDL YVRKREEALE KEKAEKLQRE
ENLRKQSEFK KRKLEEMHTD GSDEGENEEE EDEEEGQEHL PPIYVNIGGE EVNIAGTDID
PEFLDALPDD MRAEVFAQHI RERRAEALDQ TVDSREIDAD FLESIPENLR EEILEQEAAE
SRFSRLMGSL NDEENAESSG DVGEENDADE SPDSKKKKPE KIYFSPLVDR SGISAIMRSL
FISQPYLARE VYHELFYRLC SSKQNRSDIM NILMLILTEG INDHQSLERI YNLISNRALG
IAKSTSARML PLDCTPLSVA NQTIEVLQNL LETDNRLKFF FITEHENLLI NKTPLKNKKD
IFTKNMKWPI NYLFSLLDKK IITDETVLLD LLTRILQIIT KAISAIAKNE NSQKKFTVPD
IETKHLEAVV SIVKLDACNT KVFQQTLNIM THLSVIKGAQ DIFTKELCTL AIQTVEVLKT
DLSNLDSEIG EVEIGTEINY ETFQKFTVPS SEQAKLLKVL TAIDYIHTNK KKAGDIDVEK
LMGLYDQIKL GGVWTSLGHC LEKFDEKPAL SSSANILLPL IESLMVVCKH SKTRDSRDVL
KYQAKKSDFS EVAAENIFFA FTDLHKKLLN QMIRSNPKLM SGPFALLVKN PKILDFDNKR
YYFIAKLRSG AQEKPKLSIS VRREQVFLDS YRSLFFKSNE DIKNSKLEIT FKGEAGVDAG
GITREWYQVL SRQMFNPDYA LFLPVASDKT TFHPNRTSGI NPEHLSFFKF IGMIIGKAIC
DQCFLDCHFS REVYKNILSK PVALKDMESL DLDYYKSLIW ILENDITDII EETFSVETDD
YGEHKVIDLI EDGHNVPVTE QNKQEYVKKI VEYKLHTSVK EQMDNFLLGF YAIIPKDLVS
IFDEQELELL ISGLPDIDVD DWKNNTNYVN YTPTCKQISY FWRAVRSFDS EERAKLLQFV
TGTSKVPLSG FKELTGVNGI SKFSIHRDYG STERLPSSHT CFNQLDLPAY DSYETLRGSL
LLAINEGHEG FGIA
//
