ID C5DWC9_ZYGRC Unreviewed; 3277 AA.
AC C5DWC9;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 24-JAN-2024, entry version 84.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN OrderedLocusNames=ZYRO0D13794g {ECO:0000313|EMBL:CAR28098.1};
OS Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568
OS / NRRL Y-229) (Candida mogii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Zygosaccharomyces.
OX NCBI_TaxID=559307 {ECO:0000313|EMBL:CAR28098.1, ECO:0000313|Proteomes:UP000008536};
RN [1] {ECO:0000313|Proteomes:UP000008536}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 2623 / CBS 732 / BCRC 21506 / NBRC 1130 / NCYC 568 / NRRL
RC Y-229 {ECO:0000313|Proteomes:UP000008536};
RX PubMed=19525356; DOI=10.1101/gr.091546.109;
RG The Genolevures Consortium;
RA Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C.,
RA Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V.,
RA Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R., Durrens P.,
RA Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L.,
RA Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V.,
RA Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I.,
RA Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A.,
RA Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L.,
RA Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.;
RT "Comparative genomics of protoploid Saccharomycetaceae.";
RL Genome Res. 19:1696-1709(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; CU928176; CAR28098.1; -; Genomic_DNA.
DR RefSeq; XP_002497031.1; XM_002496986.1.
DR STRING; 559307.C5DWC9; -.
DR GeneID; 8204294; -.
DR KEGG; zro:ZYRO0D13794g; -.
DR HOGENOM; CLU_000215_0_1_1; -.
DR InParanoid; C5DWC9; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000008536; Chromosome D.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 2.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000008536};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 2941..3277
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1649..1670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1902..1930
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1964..2028
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2048..2086
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2138..2160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2287..2318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2411..2453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1966..1983
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2048..2080
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2429..2453
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3244
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3277 AA; 375712 MW; 9CB63A9A9241FC77 CRC64;
MVKLTRYERL QKEQSALSFK PLMDELTQCS EEEFVEKLKG IREWDRSRDD LFTWIPVLNR
IDEMLTRVVE KYSYQSIDPK KQPVKLIEMT ADEEELCVNL SQFTSRLLCN TENRFIYSSL
DVMNNLLNCP NFRVKLGATK VLAIMGERYV ITRERFDKDN ILGNPQLKRK LLRLALSIPS
STMDEERGEH FSLVELFLDK KKYPSKWSKL KYTYYTAGGG NSSNNKRAPP SSSGKEAVQS
SSMKRLVFSK EDLQSHSLQQ LFDKGMEVLP SDVWFDFSIQ VTMAKAFSDD SFENIELRKL
LIRTKFIAIA VVNTVYVPPQ VSSKLFEVDP YAFNSLTDFV SLSETKIPKE LRLDSLFALE
CTSLKHVWCS DIVRNLGGNM SHGLLFQILR YIGKILRDES QEVDEEYNVR FFYLISNLAD
VKALHESLLG AGLIPSLLDI MLVKNTTYRR TLASATHLLE AVINDGDSTA EFIGNDGFAI
LINSIMEEVD FALEHPEYGG PPKYSVVYYS VSFRQLAYIR SLLKLVLKLL KTDSGDRIRN
LIDSPILISI KKILENRPVF GYTLITYALD IVQKVINSEP TIYPILVEAG LVPYIIDHFP
EMMGPSSELL SILPDVISAL CLNSEGLKKA KENNMVRFLF DAVTNPEYAK ILIWKESATD
LGASMDELAR HYPDLKPQIL QCFHDIVKEL PKRINFKQSY LYEPSNSKEL FFQSKDEPAE
EKEENAGELA FWEVQEATPL VDCFADVIYG MSLDNSTLEN LPEKLNVRDL FSIIIMDRPP
FDYTSSQAML NFTDVLQLFD EQKKDYVFPE MMKVLQERLQ HVSEFLNFNN EESYLLRARK
DRDNSDIDGL LGEFSGLAAL LYLMTDVYVN VTSLIPARIH QILNYFDKNG FELIKLLRLL
YQKCALEEMY LTQRMPDEVL TQTMPEPLGT ASPVQIHATK PVKQAQKDDF TSAKYKNTFE
ARFLLNKLQS CTAMLFRCFL RLTHARNMEV EQEKQVVEVH VFDEVSYQMQ EMLQAVDLEK
SLPYFLVIFN FINYIYSFPK TSITGTGILQ TVPAYLFYQY GGYRFYSDAI KTLFAKMSTF
KDIAAIEEVD YIKNTDEVLT LSCLLNLLTF FNRSLSLDTM EHIPCMEVFG LVPKIGDDYV
LTKALMDLVK KMSLCLVYKL ANEGCLFNSR ARTVPYPVFK QVLTMLKNTY TNINADEDSV
LELNWDLMPP RYRVIDALKS CGLTEEQARK FITAEEHAYE SGGLNDDELF PSKPDVFSEE
GWERYKSIKL SGSLSLTPME PRHGNDMFSS DRLETMTDEF LDNGLPQKVF DVLPFYPKLV
NAIARTLLQM FSNYDEPVEN FASQVLEKIL QTNLEDGATL SSLIHIFGIF LNEKQVYENT
GRLISEFLEY LQKSLSPEYI NSSWFSKALY VYEIILAKSE VPVMESLPDD IPVRYRLPPT
PNVYRIPANI KQNIFDSLIR VGEITNFYSA LATCRILIFY ARDEGYASEI ARSGTLSKLL
KVIGIFQKSD KINFLESSFL LLVRRCFETV DIVSTLIRCE LNKSFTTRVI GDHKEKERDL
TSLIEEKPHV VMRNPDKFID NLCETARFQS FSGDGKLQEY NVHRNFDDKP SENEDVATKN
EANSAVQNRT GIIHLLLSQL MAAYKKDWTS EPNQPNDVKD TSNDKQEKVD PTKNPVCAYM
MFLLKVLIEL VSSYKQCKFE FLTFERRNQY TEFPKPKSTA LNFFLYQLLD KSTVHEQDKF
EAKRREVISM LARSVVVGFI AAVQDDNNKK SDLKAIDPDM NFIRKFTIES IMRALKNSTM
TSKLLEANVS KLETWFKVIS SMVLVQAPYL RLILDSNKLD ADQYQICKLM MDMNVPASVT
ECIAGVDLNY PFAKNLFNNA VDPLNCINSI RNTFADLFKI ESNDDEEDVD EESDKEDPPD
MFKNSALGMY DVEDIEEDDD DDVSLMGDDE DIAFVDGDDG EFEVVFSDDN EHGSGHDDHS
DIGDSGDEEM DYSSDDHDME HMHDHEDHSE EGEDEEGSGE SYYSDDSNGD IEVIDVDADD
GLEIELDDYD VDESDWESGL SELSASEEDL EDGEDGENED DANVGGLRRR WATSDGFDII
EDTSDEDEAT GVFQGLEHVF HGEGQPLIRI QDARRHGRHH NHPFRRNHGH SLLGPPSLTL
LNGGRRHQSN LINPLGPSGL EEIENGISDQ LVNVGNGARH RPEHTHFSNV LFSGEFFDER
SPDGIILKPT VSRWKDIFDM FYDTKSYASF IIPTVIAKLY GPSLRLYREA EERREQEYNQ
RLEKLREEET ERVHQTARPA SQPTAPAATS RDVEEGHEPH EPVYVTIDGT EVDIGGTDID
PEFLNALPED MRSEVFAQHV RERRAEAMRN DVHSREIDSD FLDAIPESLR EEILEQESAE
TRFSNIIHSI GEQTHEHDQD EDMLSDEGEI DDGNRPGEER RRSESDKKKP SRTYFSPLVD
RAGIAAIMKS IFISQPYVQR EMYHELFYRL CSSKQSRNEI INMLLMILTE GIIDENSLEK
VYNLISSRAN GSGKIQNSVS GGRQLPADCS PLIVANQAVE ILQNLLDSDN RLKYFFITEH
ENLMVNKAPV KNKKDIFHKN LKWPIKYLFG LLDRRIITDE SVLMDLLTRI LQVCTKPINA
LSKNSDESSG SKKKFQVPYF DRKDFERIVS IINMDSCNTR VFQQTLSVMH HLSMLKDAVK
IFTEELISLA LKTVRNLVAD LNKLTEESSM VSNGTEFNSE LVQKFTMPSS DQAKLLKVLT
AVDYLYTHKK DLSEEDVSKL MGVYNEMQLG QIWSSLSKCL FEFENRKGLN TSATVLLPLI
ESLMVVFKHI KTSQSGNKNT ALKYEDEKKL DFSNSGTESF FFKFTEAHKK LLNQMIRSNP
KLMSGPFSLL VKNPKVLDFD NKRYYFNAKL RSDAPDRPKL SISVRREQVF LDSYRALFFK
SNEDIKKSKL EITFKGESGV DAGGLTREWY QVLSRQMFNP DYALYLPVES DRTTFRPNRT
SGINPEHLSF FKFVGMVIGK AICDQCFLDC HFSREVYKNI LGRPVSLKDM ESLDLDYYKS
LIWILENDIT DIIEETFSLE TDDYGERKVV ELIPNGSEIQ VTEENKQEYV KKIVEYKLHL
SVKEQMDNFL QGFYALIPRD LISIFDEQEL ELLISGLPDV DVDDWRNNTN YVNYTANCKQ
INYFWRAVRS FDAEERAKLL QFVTGTSKVP LNGFKELTGV SGICKFSIHR DYCPTDRLPS
SHTCFNQLNL PSYNSYDTLR GSLLLAINEG HEGFGIA
//
