ID C5MCQ7_CANTT Unreviewed; 879 AA.
AC C5MCQ7;
DT 28-JUL-2009, integrated into UniProtKB/TrEMBL.
DT 28-JUL-2009, sequence version 1.
DT 24-JAN-2024, entry version 55.
DE RecName: Full=Telomerase reverse transcriptase {ECO:0000256|ARBA:ARBA00016182, ECO:0000256|RuleBase:RU365061};
DE EC=2.7.7.49 {ECO:0000256|ARBA:ARBA00012493, ECO:0000256|RuleBase:RU365061};
DE AltName: Full=Telomerase catalytic subunit {ECO:0000256|RuleBase:RU365061};
GN ORFNames=CTRG_04008 {ECO:0000313|EMBL:EER32337.1};
OS Candida tropicalis (strain ATCC MYA-3404 / T1) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=294747 {ECO:0000313|EMBL:EER32337.1, ECO:0000313|Proteomes:UP000002037};
RN [1] {ECO:0000313|EMBL:EER32337.1, ECO:0000313|Proteomes:UP000002037}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-3404 / T1 {ECO:0000313|Proteomes:UP000002037};
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W., Harris D., Hoyer L.L., Hube B., Klis F.M.,
RA Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M., Nikolaou E.,
RA Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F., Sherlock G.,
RA Shah P., Silverstein K.A., Skrzypek M.S., Soll D., Staggs R.,
RA Stansfield I., Stumpf M.P., Sudbery P.E., Srikantha T., Zeng Q., Berman J.,
RA Berriman M., Heitman J., Gow N.A., Lorenz M.C., Birren B.W., Kellis M.,
RA Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
RN [2] {ECO:0007829|PDB:6ZD1, ECO:0007829|PDB:6ZD2}
RP X-RAY CRYSTALLOGRAPHY (2.47 ANGSTROMS) OF 177-879.
RX PubMed=33856462; DOI=10.1093/nar/gkab261;
RA Zhai L.T., Rety S., Chen W.F., Song Z.Y., Auguin D., Sun B., Dou S.X.,
RA Xi X.G.;
RT "Crystal structures of N-terminally truncated telomerase reverse
RT transcriptase from fungi.";
RL Nucleic Acids Res. 49:4768-4781(2021).
CC -!- FUNCTION: Telomerase is a ribonucleoprotein enzyme essential for the
CC replication of chromosome termini in most eukaryotes. It elongates
CC telomeres. It is a reverse transcriptase that adds simple sequence
CC repeats to chromosome ends by copying a template sequence within the
CC RNA component of the enzyme. {ECO:0000256|RuleBase:RU365061}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49;
CC Evidence={ECO:0000256|ARBA:ARBA00024557,
CC ECO:0000256|RuleBase:RU365061};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365061}.
CC Chromosome, telomere {ECO:0000256|RuleBase:RU365061}.
CC -!- SIMILARITY: Belongs to the reverse transcriptase family. Telomerase
CC subfamily. {ECO:0000256|ARBA:ARBA00008001,
CC ECO:0000256|RuleBase:RU365061}.
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DR EMBL; GG692399; EER32337.1; -; Genomic_DNA.
DR RefSeq; XP_002549711.1; XM_002549665.1.
DR PDB; 6ZD1; X-ray; 2.47 A; A=177-879.
DR PDB; 6ZD2; X-ray; 2.84 A; A=158-745.
DR PDB; 6ZD6; X-ray; 2.64 A; A=177-879.
DR PDB; 6ZDP; X-ray; 2.85 A; A=158-879.
DR AlphaFoldDB; C5MCQ7; -.
DR SMR; C5MCQ7; -.
DR STRING; 294747.C5MCQ7; -.
DR EnsemblFungi; CTRG_04008-t43_1; CTRG_04008-t43_1-p1; CTRG_04008.
DR GeneID; 8296986; -.
DR KEGG; ctp:CTRG_04008; -.
DR VEuPathDB; FungiDB:CTRG_04008; -.
DR eggNOG; KOG1005; Eukaryota.
DR HOGENOM; CLU_012565_0_0_1; -.
DR OrthoDB; 55913at2759; -.
DR Proteomes; UP000002037; Unassembled WGS sequence.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003721; F:telomerase RNA reverse transcriptase activity; IEA:InterPro.
DR CDD; cd01648; TERT; 1.
DR Gene3D; 1.10.132.70; -; 1.
DR Gene3D; 3.30.70.2630; -; 1.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR021891; Telomerase_RBD.
DR InterPro; IPR003545; Telomerase_RT.
DR PANTHER; PTHR12066; TELOMERASE REVERSE TRANSCRIPTASE; 1.
DR PANTHER; PTHR12066:SF0; TELOMERASE REVERSE TRANSCRIPTASE; 1.
DR Pfam; PF12009; Telomerase_RBD; 1.
DR PRINTS; PR01365; TELOMERASERT.
DR SMART; SM00975; Telomerase_RBD; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:6ZD1, ECO:0007829|PDB:6ZD2};
KW Chromosome {ECO:0000256|ARBA:ARBA00022454, ECO:0000256|RuleBase:RU365061};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365061};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365061};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU365061};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365061};
KW Reference proteome {ECO:0000313|Proteomes:UP000002037};
KW RNA-directed DNA polymerase {ECO:0000256|RuleBase:RU365061};
KW Telomere {ECO:0000256|ARBA:ARBA00022895, ECO:0000256|RuleBase:RU365061};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365061}.
FT DOMAIN 394..734
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
SQ SEQUENCE 879 AA; 101660 MW; BB9505D19FD2FB2B CRC64;
MQTISLLQYV KDNSSDQSVQ LPSFINYLKS VNLIEITKPN KLPRITSDTS YQDFINNLIT
ELVLRKSQNV LVSGYKSTIE DSKVALPCVN LDGNFLPTNL NDSNWHRVFT LLGSEKFLNL
LINHKGFLKH KNGQQIQIFG DITSYAKPAA KAVLPIPKWI YKFDVLYQSR NPRYRNYEII
RTNVQEVLAE IVSPSTKNPN SENPAVKKRF RGIKNVVSRI ISNDKKCRYD LIYNKYLSSS
DTRKLKTMID YSTKFNRVVE VVLIIMGKLL PLDAWGGTEN KKVIQDRIVD FLRLGANERL
HLDDVLSGIK LSKFKWLGVG NNISSQQDFQ IRKRLLEGYI NWVFISLVKN IVRAFWYVTE
SSNMDRSKLF YFTHSIWNEL SSNWITKYAK GNLVQVVSPE SKGQFTNGKI KLIPKRGGFR
VICVPLKQSL YSFNNKRNFA LKQKEKWDYI FYQKYTLSPV RQVLQLKLNA LRKSDMGHRS
SVNSTNEVAD RILTFRNDLL KKNKTLPVLY MIKFDMKECY DRLNQNALKE SIAGIFKEDN
ENTTYHVREY GTLDEFLKLK RVRTLIETEV QNFNIIMNSK DEAEAGSRSY GTKVDKVKTL
SISKNKIIEV CHSQIEDATC LVKNKEGQYD LFKRKQGVFQ GFSLSGIFCD ILYSTMVSKE
FKFLWEATED NLLLRLVDDF IFITSNKDTL KKVKDKISSN ELQKYGAFVN HEKTVEINGE
AGSSNKMTFV GLDINCLTLD VKKDSSQFSR PTCKFRSFKA LFSNLKQFYC SNLSEFLLDF
SSNSLETIRE NVDAILKLTF EAIQTSFATI SKQDSFERYR FMKFLHVIIE TTIEKFARVN
GSMEGVEYLL TCIKITITKS LAFMATKQEI IEWLYTLTI
//
