ID C5NUH5_9BACL Unreviewed; 319 AA.
AC C5NUH5;
DT 01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 01-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=High-affinity heme uptake system protein IsdE {ECO:0000256|ARBA:ARBA00015862};
DE AltName: Full=Iron-regulated surface determinant protein E {ECO:0000256|ARBA:ARBA00031463};
DE AltName: Full=Staphylococcal iron-regulated protein F {ECO:0000256|ARBA:ARBA00031148};
GN Name=isdE {ECO:0000313|EMBL:EER69169.1};
GN ORFNames=GEMHA0001_0077 {ECO:0000313|EMBL:EER69169.1};
OS Gemella haemolysans ATCC 10379.
OC Bacteria; Bacillota; Bacilli; Bacillales; Gemellaceae; Gemella.
OX NCBI_TaxID=546270 {ECO:0000313|EMBL:EER69169.1, ECO:0000313|Proteomes:UP000006004};
RN [1] {ECO:0000313|EMBL:EER69169.1, ECO:0000313|Proteomes:UP000006004}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10379 {ECO:0000313|EMBL:EER69169.1,
RC ECO:0000313|Proteomes:UP000006004};
RA Fulton L., Clifton S., Chinwalla A.T., Mitreva M., Sodergren E.,
RA Weinstock G., Clifton S., Dooling D.J., Fulton B., Minx P., Pepin K.H.,
RA Johnson M., Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EER69169.1, ECO:0000313|Proteomes:UP000006004}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10379 {ECO:0000313|EMBL:EER69169.1,
RC ECO:0000313|Proteomes:UP000006004};
RA Sebastian Y., Madupu R., Durkin A.S., Torralba M., Methe B., Sutton G.G.,
RA Strausberg R.L., Nelson K.E.;
RL Submitted (JUN-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000256|ARBA:ARBA00001970};
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 8 family.
CC {ECO:0000256|ARBA:ARBA00008814}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EER69169.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ACDZ02000004; EER69169.1; -; Genomic_DNA.
DR RefSeq; WP_004392793.1; NZ_ACDZ02000004.1.
DR AlphaFoldDB; C5NUH5; -.
DR GeneID; 78010793; -.
DR eggNOG; COG0614; Bacteria.
DR OrthoDB; 66025at2; -.
DR Proteomes; UP000006004; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015886; P:heme transport; IEA:InterPro.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 2.
DR InterPro; IPR019957; ABC_transptr_haem-bd_IsdE.
DR InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR NCBIfam; TIGR03659; IsdE; 1.
DR PANTHER; PTHR30535:SF36; HIGH-AFFINITY HEME UPTAKE SYSTEM PROTEIN ISDE; 1.
DR PANTHER; PTHR30535; VITAMIN B12-BINDING PROTEIN; 1.
DR Pfam; PF01497; Peripla_BP_2; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS50983; FE_B12_PBP; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Heme {ECO:0000256|ARBA:ARBA00022617}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..319
FT /note="High-affinity heme uptake system protein IsdE"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039425844"
FT DOMAIN 65..319
FT /note="Fe/B12 periplasmic-binding"
FT /evidence="ECO:0000259|PROSITE:PS50983"
SQ SEQUENCE 319 AA; 35775 MW; F5CA648FA906F4A2 CRC64;
MKRKLSLLFT LLLTSILVLT ACSSQNKAGN TTGTDPNLAD KAFNETIDLT GVNKNKKSEE
ENGKRVVMDS VALAQVANVL DIKLAAVPTT KLGRIPKKYD NTVQIGLPMN PNMEVIKSIN
PSIVYAPDSL QDWIKEGFEK NKIPYKFIDI RSVNGLYAVT EELAKEFGKT EKFKELKKEH
DKFFAKFNEK IANKKKPKVL VLMGLPGSYM AATEKSYVGN LVKLAGGENI VKGNEEFKNL
NLETALNDKP DFILRTAHAM PDTVNEMFKK EFENNKSWSH FDAVKNNKVV DLDSSMFGMT
ATYDYQKGLE SLYSIFYSN
//
