ID C6A058_THESM Unreviewed; 526 AA.
AC C6A058;
DT 01-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 01-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 78.
DE RecName: Full=DNA repair and recombination protein RadA {ECO:0000256|ARBA:ARBA00018144, ECO:0000256|HAMAP-Rule:MF_00348};
GN Name=radA {ECO:0000256|HAMAP-Rule:MF_00348};
GN OrderedLocusNames=TSIB_1992 {ECO:0000313|EMBL:ACS91039.1};
OS Thermococcus sibiricus (strain DSM 12597 / MM 739).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=604354 {ECO:0000313|EMBL:ACS91039.1, ECO:0000313|Proteomes:UP000009079};
RN [1] {ECO:0000313|EMBL:ACS91039.1, ECO:0000313|Proteomes:UP000009079}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 12597 / MM 739 {ECO:0000313|Proteomes:UP000009079};
RX PubMed=19447963; DOI=10.1128/AEM.00718-09;
RA Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V.,
RA Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G.;
RT "Metabolic versatility and indigenous origin of the archaeon Thermococcus
RT sibiricus, isolated from a siberian oil reservoir, as revealed by genome
RT analysis.";
RL Appl. Environ. Microbiol. 75:4580-4588(2009).
CC -!- FUNCTION: Involved in DNA repair and in homologous recombination. Binds
CC and assemble on single-stranded DNA to form a nucleoprotein filament.
CC Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand
CC exchange between homologous DNA molecules. {ECO:0000256|HAMAP-
CC Rule:MF_00348}.
CC -!- SIMILARITY: Belongs to the eukaryotic RecA-like protein family.
CC {ECO:0000256|ARBA:ARBA00008050, ECO:0000256|HAMAP-Rule:MF_00348}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00348}.
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DR EMBL; CP001463; ACS91039.1; -; Genomic_DNA.
DR AlphaFoldDB; C6A058; -.
DR STRING; 604354.TSIB_1992; -.
DR KEGG; tsi:TSIB_1992; -.
DR eggNOG; arCOG00415; Archaea.
DR HOGENOM; CLU_528557_0_0_2; -.
DR Proteomes; UP000009079; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_00348; RadA_arch; 1.
DR InterPro; IPR013632; DNA_recomb/repair_Rad51_C.
DR InterPro; IPR011938; DNA_recomb/repair_RadA.
DR InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020588; RecA_ATP-bd.
DR InterPro; IPR020587; RecA_monomer-monomer_interface.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR22942:SF39; DNA REPAIR PROTEIN RAD51 HOMOLOG 1; 1.
DR PANTHER; PTHR22942; RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF08423; Rad51; 2.
DR SMART; SM00278; HhH1; 2.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR SUPFAM; SSF47794; Rad51 N-terminal domain-like; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS50162; RECA_2; 1.
DR PROSITE; PS50163; RECA_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00348}; Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA damage {ECO:0000256|HAMAP-Rule:MF_00348};
KW DNA recombination {ECO:0000256|HAMAP-Rule:MF_00348};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00348};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00348}; Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000009079}.
FT DOMAIN 299..322
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 322..462
FT /note="RecA family profile 1"
FT /evidence="ECO:0000259|PROSITE:PS50162"
FT DOMAIN 467..526
FT /note="RecA family profile 2"
FT /evidence="ECO:0000259|PROSITE:PS50163"
SQ SEQUENCE 526 AA; 58357 MW; 8A43E9221DDB372E CRC64;
MIQMARKKKI EDEVKELDEF EELDALDELS VEEPTKGSNK KEIKSIEDLP GVGPATAEKL
REAGYDSLEA IAVASPLELK ELAGVSEGAA LKIIQAAREA ANIGTFMRAD EYFQKRAVVG
KITTGSKSLD KLLGGGIETQ AVSEVFGEFG SGKCFAKDTT VYYENDDVAH VESIEEMYNK
YATKNGEIPF DNGFAVPLEV VSVYTFNIKT RKVEKTKVSY IYKEKVSTLV KLKLSTGIEL
KVTQSHPVLV FKDGLKWIKA SEVQIGDRVV GIGEVPPKSE VNLRFHQVES VEIFDYNDYV
YDLVVPETHN FIAPNGLILH NTQLAHTLAV IVQKPPEEGG LNGSVVWIDT ENTFRPERIK
QIAESRGMDP DEVLKNIYVA RAFNSNHQML LVEKAEEIIK EKLPSEKPVK LLVVDSLTGH
FRSEYVGRGT LAERQQKLAK HLADLHRLAN LYDIAVFVTN QVQARPDAFF GDPTRPIGGH
ILAHSATVRV YLRKGKAGKR VARLIDSPHL PEGEAAFAIT EKGVED
//
