ID C6WNK1_ACTMD Unreviewed; 677 AA.
AC C6WNK1;
DT 22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT 22-SEP-2009, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN OrderedLocusNames=Amir_0961 {ECO:0000313|EMBL:ACU34920.1};
OS Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / JCM 3225 / NBRC 14064
OS / NCIMB 13271 / NRRL B-12336 / IMRU 3971 / 101).
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Actinosynnema.
OX NCBI_TaxID=446462 {ECO:0000313|EMBL:ACU34920.1, ECO:0000313|Proteomes:UP000002213};
RN [1] {ECO:0000313|EMBL:ACU34920.1, ECO:0000313|Proteomes:UP000002213}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29888 / DSM 43827 / JCM 3225 / NBRC 14064 / NCIMB 13271 /
RC NRRL B-12336 / IMRU 3971 / 101 {ECO:0000313|Proteomes:UP000002213};
RX PubMed=21304636; DOI=10.4056/sigs.21137;
RA Land M., Lapidus A., Mayilraj S., Chen F., Copeland A., Del Rio T.G.,
RA Nolan M., Lucas S., Tice H., Cheng J.F., Chertkov O., Bruce D., Goodwin L.,
RA Pitluck S., Rohde M., Goker M., Pati A., Ivanova N., Mavromatis K.,
RA Chen A., Palaniappan K., Hauser L., Chang Y.J., Jeffries C.C., Brettin T.,
RA Detter J.C., Han C., Chain P., Tindall B.J., Bristow J., Eisen J.A.,
RA Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Actinosynnema mirum type strain (101).";
RL Stand. Genomic Sci. 1:46-53(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP001630; ACU34920.1; -; Genomic_DNA.
DR RefSeq; WP_012783582.1; NC_013093.1.
DR AlphaFoldDB; C6WNK1; -.
DR STRING; 446462.Amir_0961; -.
DR KEGG; ami:Amir_0961; -.
DR eggNOG; COG0286; Bacteria.
DR eggNOG; COG0732; Bacteria.
DR HOGENOM; CLU_022127_0_0_11; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000002213; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.90.220.20; DNA methylase specificity domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR044946; Restrct_endonuc_typeI_TRD_sf.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR42998:SF1; TYPE I RESTRICTION ENZYME HINDI METHYLASE SUBUNIT; 1.
DR PANTHER; PTHR42998; TYPE I RESTRICTION ENZYME HINDVIIP M PROTEIN-RELATED; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF116734; DNA methylase specificity domain; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000313|EMBL:ACU34920.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002213};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW Transferase {ECO:0000313|EMBL:ACU34920.1}.
FT DOMAIN 122..354
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT REGION 348..370
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 677 AA; 71885 MW; A5B23419B3C528DF CRC64;
MTEEAAVTAA EIARLSGVGR AAVSNWRRRH EDFPQPAGGT DTSPVFKLSE VQAWLQGQGK
LNALSGRELV WQHLEAASGT DLISDALAAA GTYLANGSSG KMFDENTRNA LDALASEDGA
EAAFEQLHDR FVEANWRGVA VTPRELARLM VDLTEPHGTV FDPACGTGAL LRAAVRSEPG
IRPVGQELDP SLAQVAIARL AFAAGEASVR SGDSLRNDAF PELVADVVVS NPPFNIRNWG
AEELAYDRRW VYGVPPKGES ELAWVQHCLA HLRPGGHAVV LMPPAVASRR SGRPIRAELL
RSGTLRAVVA LPPGAAAPLH VGLQIWVLRR PEPGGADRRT VLFIDTAGEQ HTHPPEAHRG
STGRTSPRVQ AGRQVVGWDT LSATVLGHWR AFSPNPECFT DAPGTTRAIP AIDLLDDLVD
VSPARHVRIS ASTADPIAVA KHANELRDRL TESATTLAAS PIPAGWRPVG EESRSWRTAT
VADLSRGGAL TLHRTVPAVR GSKEINLPEE PASRRVLTGR DVATDARASA GADEQRSAIF
VLIEPGDVLL SGVAAGRGAA ARVADGEDAG CLLGLHVHLF RPAPDRLDPW FLAGFLSAAD
NVSSASTGTT AVHIDPRRLR VPLLPLIEQR RYGGAFRQMH ELRTTARQVA KLATETAGTL
AAGLTSGALA PPDVDCP
//
