ID   C7BGS2_PHOAA            Unreviewed;       393 AA.
AC   C7BGS2;
DT   22-SEP-2009, integrated into UniProtKB/TrEMBL.
DT   22-SEP-2009, sequence version 1.
DT   27-MAR-2024, entry version 80.
DE   SubName: Full=Cysteine desulfurase {ECO:0000313|EMBL:CAQ82251.1};
DE            EC=4.4.1.- {ECO:0000313|EMBL:CAQ82251.1};
GN   Name=iscS {ECO:0000313|EMBL:CAQ82251.1};
GN   OrderedLocusNames=PAU_00158 {ECO:0000313|EMBL:CAQ82251.1};
OS   Photorhabdus asymbiotica subsp. asymbiotica (strain ATCC 43949 / 3105-77)
OS   (Xenorhabdus luminescens (strain 2)).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=553480 {ECO:0000313|EMBL:CAQ82251.1, ECO:0000313|Proteomes:UP000002747};
RN   [1] {ECO:0000313|EMBL:CAQ82251.1, ECO:0000313|Proteomes:UP000002747}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43949 / 3105-77 {ECO:0000313|Proteomes:UP000002747};
RX   PubMed=19583835; DOI=10.1186/1471-2164-10-302;
RA   Wilkinson P., Waterfield N.R., Crossman L., Corton C.,
RA   Sanchez-Contreras M., Vlisidou I., Barron A., Bignell A., Clark L.,
RA   Ormond D., Mayho M., Bason N., Smith F., Simmonds M., Churcher C.,
RA   Harris D., Thompson N.R., Quail M., Parkhill J., ffrench-Constant R.H.;
RT   "Comparative genomics of the emerging human pathogen Photorhabdus
RT   asymbiotica with the insect pathogen Photorhabdus luminescens.";
RL   BMC Genomics 10:302-302(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-
CC         alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA-
CC         COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972,
CC         ChEBI:CHEBI:64428; EC=2.8.1.7;
CC         Evidence={ECO:0000256|ARBA:ARBA00001357};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933};
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. NifS/IscS subfamily.
CC       {ECO:0000256|ARBA:ARBA00006490}.
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DR   EMBL; FM162591; CAQ82251.1; -; Genomic_DNA.
DR   AlphaFoldDB; C7BGS2; -.
DR   STRING; 291112.PAU_00158; -.
DR   KEGG; pay:PAU_00158; -.
DR   eggNOG; COG1104; Bacteria.
DR   Proteomes; UP000002747; Chromosome.
DR   GO; GO:0031071; F:cysteine desulfurase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR   Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR016454; Cysteine_dSase.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR   PANTHER; PTHR11601:SF34; CYSTEINE DESULFURASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR11601; CYSTEINE DESULFURYLASE FAMILY MEMBER; 1.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   PIRSF; PIRSF005572; NifS; 1.
DR   SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE   3: Inferred from homology;
KW   2Fe-2S {ECO:0000256|ARBA:ARBA00022714};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Lyase {ECO:0000313|EMBL:CAQ82251.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898}.
FT   DOMAIN          8..370
FT                   /note="Aminotransferase class V"
FT                   /evidence="ECO:0000259|Pfam:PF00266"
SQ   SEQUENCE   393 AA;  42491 MW;  B8770A077F5CFC84 CRC64;
     MYNLARPIYL DNNATTPLDP DVLQSMMPYF TNAYGNASSN NHVFGWKAME AVDDARQVIS
     TSINATSHAD IIFTSGATES NNLAIMGLQH RGKGGHVITS TIEHKAVLDC CGRLESMGTE
     VTYLPPQSDG IVSPDSISRA IRDDTYLVSI MAANNEIGSI QKIEEIGAIC KARDIPLHTD
     ATQALGKIEF DVQRMNVSLA SFSAHKIYGP KGIGALYINS RDKNISLTPL IVGGGHERGL
     RSGTLNVPGI VGFGRAVELS VKNLTAESMR LRYLRDLLRL YIIENVADVR INGHLTECLP
     GLLNVSFNGI DADSLLLELS EVALSSGSAC TSANRAPSHV LKAIGVSDAL AQASVRFGLG
     RFNNEDEIRY VCLRLEKAVN KLRSMAPKSF VTQ
//