ID C7NIM2_KYTSD Unreviewed; 1183 AA.
AC C7NIM2;
DT 13-OCT-2009, integrated into UniProtKB/TrEMBL.
DT 13-OCT-2009, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN OrderedLocusNames=Ksed_16450 {ECO:0000313|EMBL:ACV06660.1};
OS Kytococcus sedentarius (strain ATCC 14392 / DSM 20547 / CCM 314 / 541)
OS (Micrococcus sedentarius).
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Kytococcaceae;
OC Kytococcus.
OX NCBI_TaxID=478801 {ECO:0000313|EMBL:ACV06660.1, ECO:0000313|Proteomes:UP000006666};
RN [1] {ECO:0000313|EMBL:ACV06660.1, ECO:0000313|Proteomes:UP000006666}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14392 / DSM 20547 / CCM 314 / 541
RC {ECO:0000313|Proteomes:UP000006666};
RX PubMed=21304632; DOI=10.4056/sigs.761;
RA Sims D., Brettin T., Detter J.C., Han C., Lapidus A., Copeland A.,
RA Glavina Del Rio T., Nolan M., Chen F., Lucas S., Tice H., Cheng J.F.,
RA Bruce D., Goodwin L., Pitluck S., Ovchinnikova G., Pati A., Ivanova N.,
RA Mavrommatis K., Chen A., Palaniappan K., D'haeseleer P., Chain P.,
RA Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Schneider S.,
RA Goker M., Pukall R., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Kytococcus sedentarius type strain (541).";
RL Stand. Genomic Sci. 1:12-20(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001686; ACV06660.1; -; Genomic_DNA.
DR RefSeq; WP_015779605.1; NC_013169.1.
DR AlphaFoldDB; C7NIM2; -.
DR STRING; 478801.Ksed_16450; -.
DR KEGG; kse:Ksed_16450; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_0_0_11; -.
DR Proteomes; UP000006666; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 11..78
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1183 AA; 131068 MW; 9AFC04243EA723CE CRC64;
MSAPGSAENF VHLHNHTEYS MLDGAARIDD MFTVADEMGM PAIASTDHGS VFGAYEFWSK
SRKFETKPIV GVEAYLTPGT HRTDKTRVKF GDGGRNDVSG SGAYTHMTMW AETTEGMHNL
FRASSLASIE GFYFKPRMDR ELLDRYGKGI IATTGCPSGE VQTRLRLGQY DKAKEAAAEF
RDIFGKDNFF CELMDHGLDI ERQVRADLLK LAKELDLPLV ATNDLHYTHK EDAKAHGALL
CVQSGSTLMD PNRFKFDSDE FYLKSAAEMR HLWREVPEAC DNTLLIAERC NVEFTEGEGR
YMPRFPCPDG HDETSWFISE VQTGLARRFP EGVPDYALKQ AAYEQDVIIN KGYPGYFLVV
ADFINWAKEN GIRVGPGRGS GAGSMCAYAM GITDLDPIPH GLIFERFLNP ERASMPDFDV
DFDERRRPEV IKYVTEKYGS DRVAMIVTYG TIKAKQALKD ASRVMGFPFA TGEKLTKAMP
PAVMGKDIPL SGIFDEKHPR YAEAIDFREL HANDPEAREV FDTAVGLEGL KRQWGVHAAG
VIMASEPLID LIPIMRREAD GQIITQFDYP SCETLGLVKM DFLGLRNLTI LDDAIANIEM
NREETIDLDA LSKDMTDPAA YKLLARGDTL GVFQLDGGGM RSLLRLMQPD NFEDISAALA
LYRPGPMGAN AHTNFALRKN GKQPVDYIHP ELTEALEPIL KMTYGLIIYQ EQVMEIAQKL
AGYTLGNADL LRRAMGKKKK EVLDAEYVNF EAGMLANGYS KDAVETLWGI LLPFSDYAFN
KAHTAAYGVV SYWTAYLKAN YRAEYMAALL TSVRDDKDKS ALYLGECRRM GIKVLPPDVN
ESIANFAAVG QDIRFGLEAI RNVGGNVVRA IVATREEKGK FTSFEDFLRK CPAVVCNKRT
IDSLIKAGAF DDLGHQRQAL ATVHEAYVDA LVGEKKEQER GQESLFASLM GDGGDDASSI
GGMPAIPDLE WDKQTLLAFE REMLGLYVSD HPLFGIEHAL SRASDTSIAH LNSEDGRPDG
AEVTIAGLIT QVVTKRNKRG ELWAIATVED LEGSIECLFF ARTYMTVSTM LTQDVVCVVS
GRVKRGDDAV SIFASDLRLP DLDDGPRGPV MIHMAMNQTT DAVVGKLRNV LSDHPGPTEV
QIHLTQPGRR VVMRVEDSLR VEASNSLFGD LKQLLGPNCL PVA
//
