ID C8NIF4_9LACT Unreviewed; 969 AA.
AC C8NIF4;
DT 03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 03-NOV-2009, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN Name=hsdR {ECO:0000313|EMBL:EEW36580.1};
GN ORFNames=HMPREF0444_1699 {ECO:0000313|EMBL:EEW36580.1};
OS Granulicatella adiacens ATCC 49175.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
OC Granulicatella.
OX NCBI_TaxID=638301 {ECO:0000313|EMBL:EEW36580.1, ECO:0000313|Proteomes:UP000005926};
RN [1] {ECO:0000313|EMBL:EEW36580.1, ECO:0000313|Proteomes:UP000005926}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49175 {ECO:0000313|EMBL:EEW36580.1,
RC ECO:0000313|Proteomes:UP000005926};
RA Muzny D., Qin X., Deng J., Jiang H., Liu Y., Qu J., Song X.-Z., Zhang L.,
RA Thornton R., Coyle M., Francisco L., Jackson L., Javaid M., Korchina V.,
RA Kovar C., Mata R., Mathew T., Ngo R., Nguyen L., Nguyen N., Okwuonu G.,
RA Ongeri F., Pham C., Simmons D., Wilczek-Boney K., Hale W., Jakkamsetti A.,
RA Pham P., Ruth R., San Lucas F., Warren J., Zhang J., Zhao Z., Zhou C.,
RA Zhu D., Lee S., Bess C., Blankenburg K., Forbes L., Fu Q., Gubbala S.,
RA Hirani K., Jayaseelan J.C., Lara F., Munidasa M., Palculict T., Patil S.,
RA Pu L.-L., Saada N., Tang L., Weissenberger G., Zhu Y., Hemphill L.,
RA Shang Y., Youmans B., Ayvaz T., Ross M., Santibanez J., Aqrawi P.,
RA Gross S., Joshi V., Fowler G., Nazareth L., Reid J., Worley K.,
RA Petrosino J., Highlander S., Gibbs R.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEW36580.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ACKZ01000028; EEW36580.1; -; Genomic_DNA.
DR RefSeq; WP_005606258.1; NZ_GG694016.1.
DR AlphaFoldDB; C8NIF4; -.
DR STRING; 638301.HMPREF0444_1699; -.
DR eggNOG; COG0610; Bacteria.
DR HOGENOM; CLU_010804_0_0_9; -.
DR Proteomes; UP000005926; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR CDD; cd22332; HsdR_N; 1.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR040980; SWI2_SNF2.
DR PANTHER; PTHR42927; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42927:SF1; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:EEW36580.1};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000005926};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 293..486
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 969 AA; 111585 MW; 2B5D642932549F8D CRC64;
MGIKDTKMEA ELENNIIEYL VSNQGYKYIK PDEMKISFNR KYAFDEKRLL EFIKISQPNE
FDILRLDTES GKEKFYKQLD TSIRQNGIVS VLKNGIKCYP SSGTIIFYHA LDPKRPSSYD
EFNTNIFSAT NQLTYSEKNR GLELDLAIFV NGLPVITMEL KSRASSTGWT YKDAEEQYKN
DRDSKEALFN FKRCIAHFAV DENFITFTTK IDGKNTRFMP FNKGTVQGGS GNPINNSGTM
TDYLWKDFLQ KETLTSLIRD FAYVSVDKVK KTETLIFPRY HQYRVVTKLV DDVKNNGVGN
RYLIQHSAGS GKSNSITWLA YRLVEVNYNG QKAFDSVIVV TDRLNLDKQI NDNIRKFIDE
KSIVGHAGSS TDLKNMLIDG KKIIITTVQK FPYLLEKIGT DLKGKNFAIV IDEAHSSQSG
KAAASLNMAV SGSLGNEAEF EIEDKLNELI EARKMPENAS FFAFTATPKA KTIQMFGSVF
DLYSMKQAIE EGFILDVLKN YTHYENYYKI YKTVEDNPEF DKRKAQKKIR NYVEGQEFPI
REKAEVMINH ILTNTTNKIN GKAKAMIITQ SIRRAIEYYH IVKELLENSN TGYEALIAFS
GEKEYNGKIV TETSLNGFSD KETTEKFKQD KYRILIVADK YQTGYDEPLL HTMYVDKPLN
DVKAVQTLSR LNRSAKYKID TCVIDFANQP EHIMEAFQPY YKETKLEEET DPNKLHNLLS
LLDSKYVYEE DEVDNFVELF LNNSPRSSID SIVDKCVERY ISLSEDDQVE FKSGVKSFIR
TYNFLASILP IGQVDWEKKV IFFEQLIHRL PTPEGKDLSA GILESVDLES YRLEKKKTID
IILEDEDGKV EGLSIGAGKK NSVELDSLEN IVSTFNDIFG NIEWQDKDNV ARQIKELPAM
VMKNEKFKNA LKNSDLENIK REYQSALKEV FRIIMIDNME LFGQWTNNSN FSKWLNNLIF
DEIMKKYKK
//
