ID C8PCS7_9LACO Unreviewed; 563 AA.
AC C8PCS7;
DT 03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 03-NOV-2009, sequence version 1.
DT 24-JAN-2024, entry version 63.
DE RecName: Full=PTS system lactose-specific EIICB component {ECO:0000256|ARBA:ARBA00020834};
DE EC=2.7.1.207 {ECO:0000256|ARBA:ARBA00012802};
DE AltName: Full=EIICB-Lac {ECO:0000256|ARBA:ARBA00029639};
GN Name=lacE {ECO:0000313|EMBL:EEW51735.1};
GN ORFNames=HMPREF0520_0897 {ECO:0000313|EMBL:EEW51735.1};
OS Lactobacillus iners DSM 13335.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=525328 {ECO:0000313|EMBL:EEW51735.1, ECO:0000313|Proteomes:UP000004115};
RN [1] {ECO:0000313|EMBL:EEW51735.1, ECO:0000313|Proteomes:UP000004115}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 13335 {ECO:0000313|EMBL:EEW51735.1,
RC ECO:0000313|Proteomes:UP000004115};
RA Qin X., Bachman B., Battles P., Bell A., Bess C., Bickham C., Chaboub L.,
RA Chen D., Coyle M., Deiros D.R., Dinh H., Forbes L., Fowler G.,
RA Francisco L., Fu Q., Gubbala S., Hale W., Han Y., Hemphill L.,
RA Highlander S.K., Hirani K., Hogues M., Jackson L., Jakkamsetti A.,
RA Javaid M., Jiang H., Korchina V., Kovar C., Lara F., Lee S., Mata R.,
RA Mathew T., Moen C., Morales K., Munidasa M., Nazareth L., Ngo R.,
RA Nguyen L., Okwuonu G., Ongeri F., Patil S., Petrosino J., Pham C., Pham P.,
RA Pu L.-L., Puazo M., Raj R., Reid J., Rouhana J., Saada N., Shang Y.,
RA Simmons D., Thornton R., Warren J., Weissenberger G., Zhang J., Zhang L.,
RA Zhou C., Zhu D., Muzny D., Worley K., Gibbs R.;
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=lactose(out) + N(pros)-phospho-L-histidyl-[protein] = L-
CC histidyl-[protein] + lactose 6-phosphate(in); Xref=Rhea:RHEA:42400,
CC Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:17716,
CC ChEBI:CHEBI:29979, ChEBI:CHEBI:64837, ChEBI:CHEBI:79080;
CC EC=2.7.1.207; Evidence={ECO:0000256|ARBA:ARBA00001568};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEW51735.1}.
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DR EMBL; ACLN01000010; EEW51735.1; -; Genomic_DNA.
DR RefSeq; WP_006728941.1; NZ_GG700801.1.
DR AlphaFoldDB; C8PCS7; -.
DR GeneID; 69785394; -.
DR PATRIC; fig|525328.13.peg.1158; -.
DR HOGENOM; CLU_029688_0_0_9; -.
DR OrthoDB; 1550290at2; -.
DR Proteomes; UP000004115; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0022869; F:protein-N(PI)-phosphohistidine-lactose phosphotransferase system transporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05565; PTS_IIB_lactose; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR InterPro; IPR004801; LacE.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR013012; PTS_EIIB_3.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR004501; PTS_EIIC_3.
DR InterPro; IPR041713; PTS_IIB.
DR NCBIfam; TIGR00394; lac_pts_IIC; 1.
DR NCBIfam; TIGR00410; lacE; 1.
DR NCBIfam; TIGR00853; pts-lac; 1.
DR PANTHER; PTHR33989; -; 1.
DR PANTHER; PTHR33989:SF8; PERMEASE IIC COMPONENT; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; PTS system IIB component-like; 1.
DR PROSITE; PS51100; PTS_EIIB_TYPE_3; 1.
DR PROSITE; PS51105; PTS_EIIC_TYPE_3; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000004115};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EEW51735.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 102..120
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 132..154
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 221..238
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 281..303
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 378..406
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 8..408
FT /note="PTS EIIC type-3"
FT /evidence="ECO:0000259|PROSITE:PS51105"
FT DOMAIN 460..563
FT /note="PTS EIIB type-3"
FT /evidence="ECO:0000259|PROSITE:PS51100"
FT MOD_RES 467
FT /note="Phosphocysteine; by EIIA"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00423"
SQ SEQUENCE 563 AA; 61253 MW; 2B195F2DE42F6707 CRC64;
MNVLISKIEK GKPFFEKVSR NIYLRAIRDG FIASMPVILF SSIFLLIAFV PNIFGIRWGK
DVVELLMKPY NYTMGIVAFL VAGTTAKSLT DSINRDMDKT NQVNFISTML ASITGFLILA
SDSIQGGFSS DFLGTKGLLS AFIAAFIVVN IYKICIKKNV TIRMPEEVPP NISQVFKDVI
PFALSILVLY ALDLLVRRSL GTGVAPAIGK VIAPLFKAAD GYVGITIIFG AYALFWFIGI
HGPSIVEPAI AAITYANIET NFKLLQAGQH ADKILTSGTQ MFIVTMGGTG ATLVVPFMFM
WLAKSKQNRA IGRASVVPTF FGVNEPILFG APLVLNPIFF IPFVFAPIAN VWIFKFFVDV
LGMNSFSVNL PWTTPGPIGI IMGTGFGLWS VVLAITLIAV DVIIYYPFFK VYDNQILEHE
ISGNCSNNLK DKVAANFDTS KADAILEKNN ISKVGNDVKQ TNVLVLCAGG GTSGLLANAL
NKAAKDYNVP ITAAAGSYGS HREILPQYQL VILAPQVASN YVDMKAETDR LGIKLAKTEG
VQYIKLTRDG LGALKFVEEQ IKN
//
