ID C8VW97_DESAS Unreviewed; 488 AA.
AC C8VW97;
DT 03-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 03-NOV-2009, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE SubName: Full=Radical SAM domain protein {ECO:0000313|EMBL:ACV62449.1};
GN OrderedLocusNames=Dtox_1590 {ECO:0000313|EMBL:ACV62449.1};
OS Desulfofarcimen acetoxidans (strain ATCC 49208 / DSM 771 / KCTC 5769 / VKM
OS B-1644 / 5575) (Desulfotomaculum acetoxidans).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae;
OC Desulfofarcimen.
OX NCBI_TaxID=485916 {ECO:0000313|EMBL:ACV62449.1, ECO:0000313|Proteomes:UP000002217};
RN [1] {ECO:0000313|EMBL:ACV62449.1, ECO:0000313|Proteomes:UP000002217}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49208 / DSM 771 / VKM B-1644
RC {ECO:0000313|Proteomes:UP000002217};
RX PubMed=21304664; DOI=10.4056/sigs.39508;
RA Spring S., Lapidus A., Schroder M., Gleim D., Sims D., Meincke L.,
RA Glavina Del Rio T., Tice H., Copeland A., Cheng J.F., Lucas S., Chen F.,
RA Nolan M., Bruce D., Goodwin L., Pitluck S., Ivanova N., Mavromatis K.,
RA Mikhailova N., Pati A., Chen A., Palaniappan K., Land M., Hauser L.,
RA Chang Y.J., Jeffries C.D., Chain P., Saunders E., Brettin T., Detter J.C.,
RA Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA Kyrpides N.C., Klenk H.P., Han C.;
RT "Complete genome sequence of Desulfotomaculum acetoxidans type strain
RT (5575).";
RL Stand. Genomic Sci. 1:242-253(2009).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP001720; ACV62449.1; -; Genomic_DNA.
DR RefSeq; WP_015757160.1; NC_013216.1.
DR AlphaFoldDB; C8VW97; -.
DR STRING; 485916.Dtox_1590; -.
DR KEGG; dae:Dtox_1590; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_021572_4_3_9; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000002217; Chromosome.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF17; MG-PROTOPORPHYRIN IX MONOMETHYL ESTER OXIDATIVE CYCLASE 66KD SUBUNIT; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002217}.
FT DOMAIN 8..148
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 195..418
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 488 AA; 55857 MW; 41F44B9DAAC18B0B CRC64;
MRVLLIYPCL RLNRTPGPDW LPLGLSFMAA VLRQEGHIVS IFDRFSIQAV VGRNKDKINA
AMLEHIKVYK PDLIGLNTIS PLIYDTLECV SLIRRIYAGP VVAGGHHVSA LPELTLLKIP
GLNGIVQGEG ESAINSLARG ENPASIPGVW WKNEESIIVH TPAQQIDNLD TLPLPALDLL
DMPFYTRPTM NTIRGHYLST VSLLTSRGCN YRCDYCAESL TYGRGVRFHS PDYVIEWIKQ
VRKDYRVNGI YFHDNDFLIY ENRSREICER IISAGLSGKI KWAIQTRVNR LKPDMIKLLK
RAGCILVEVG VEAASQRELD LVNKQTTVYA NEQAIALCRN EGISIHAYML TALEGEIIKD
LEQRIQWVKR VKPSTFDWHP LKIHPGTLLY QKKGDGFFEA SEWTEENIVN YYKKDTLSCI
SQEKRREWMK RYYIRYHKRH HRMHILKVNA PIKLLQIFVK KIRASTLNLL GKISAVLANL
FNKSLTKP
//
