ID CAPP_RHOPA Reviewed; 936 AA.
AC O32483;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 16-FEB-2004, sequence version 2.
DT 27-MAR-2024, entry version 126.
DE RecName: Full=Phosphoenolpyruvate carboxylase;
DE Short=PEPC;
DE Short=PEPCase;
DE EC=4.1.1.31;
GN Name=ppc; OrderedLocusNames=RPA1772;
OS Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Rhodopseudomonas.
OX NCBI_TaxID=258594;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=7;
RX PubMed=9244286; DOI=10.1128/jb.179.15.4942-4945.1997;
RA Inui M., Dumay V., Zahn K., Yamagata H., Yukawa H.;
RT "Structural and functional analysis of the phosphoenolpyruvate carboxylase
RT gene from the purple nonsulfur bacterium Rhodopseudomonas palustris No.
RT 7.";
RL J. Bacteriol. 179:4942-4945(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-98 / CGA009;
RX PubMed=14704707; DOI=10.1038/nbt923;
RA Larimer F.W., Chain P., Hauser L., Lamerdin J.E., Malfatti S., Do L.,
RA Land M.L., Pelletier D.A., Beatty J.T., Lang A.S., Tabita F.R.,
RA Gibson J.L., Hanson T.E., Bobst C., Torres y Torres J.L., Peres C.,
RA Harrison F.H., Gibson J., Harwood C.S.;
RT "Complete genome sequence of the metabolically versatile photosynthetic
RT bacterium Rhodopseudomonas palustris.";
RL Nat. Biotechnol. 22:55-61(2004).
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000305}.
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DR EMBL; D89668; BAA21835.1; -; Genomic_DNA.
DR EMBL; BX572598; CAE27213.1; -; Genomic_DNA.
DR RefSeq; WP_011157278.1; NZ_CP116810.1.
DR AlphaFoldDB; O32483; -.
DR SMR; O32483; -.
DR STRING; 258594.RPA1772; -.
DR GeneID; 66892807; -.
DR eggNOG; COG2352; Bacteria.
DR HOGENOM; CLU_006557_2_0_5; -.
DR PhylomeDB; O32483; -.
DR Proteomes; UP000001426; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation; Lyase; Magnesium; Reference proteome.
FT CHAIN 1..936
FT /note="Phosphoenolpyruvate carboxylase"
FT /id="PRO_0000166618"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 164
FT /evidence="ECO:0000250"
FT ACT_SITE 598
FT /evidence="ECO:0000250"
FT CONFLICT 51
FT /note="S -> T (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="A -> E (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 88
FT /note="Q -> P (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="G -> S (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 198
FT /note="T -> A (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 263..266
FT /note="ANAE -> VNSD (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 311
FT /note="E -> D (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 404..406
FT /note="THH -> QHN (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="G -> T (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 460
FT /note="V -> M (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 464
FT /note="D -> E (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 530
FT /note="L -> V (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 671
FT /note="S -> R (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 798
FT /note="E -> A (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 802
FT /note="K -> T (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 809
FT /note="A -> K (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 846..850
FT /note="TAIRD -> VDVRE (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 854
FT /note="S -> G (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 865
FT /note="D -> E (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 868
FT /note="L -> F (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
FT CONFLICT 875
FT /note="H -> R (in Ref. 1; BAA21835)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 936 AA; 104139 MW; F95CF9565356EA4F CRC64;
MSSLNLSAGP EPVSERPDDA AAIEAETRLR NDIRLLGRIL GDTVREQEGQ SVFDLVENIR
QTSIRFHRDD DKTARAELAA ILDGMSIQDT MRIVRAFSYF SHLANIAEDQ NNIRQMRAGS
TAGSAPRAGL LAKTLAHARQ EGISAAELRK FFATALVSPV LTAHPTEVRR KSTMDREMQI
AGLLDQRDRV QLTADEWTDN EERLRRAVET LWKTNLLRRT KLTVLDEVTN GLSFYDYTFL
REVPRLHSAL EDRLADAAKA EGANAEGELA SFLRMGSWIG GDRDGNPFVT AEVLHGTLKL
QSTRVLRYYL EELHELGSEL SLASHLAGTT DTVKALAETS PDTSPHRKYE PYRLAVSGIY
ARLAATALKL EVENLRAPVG EAEPYASAQD FKADLDAIHL SLTTHHSGVI ARGRLRQLRR
AIDCFGFHLA SLDMRQNSAV HERTIGELMD AARPGTSYAV LDEDARIALL ISELRSTRPL
TSMFVKYSDE TVGELAVFRE AAKAHATYGA AAIPQCIISM TKGVSDLLEL AVLLKEVGLI
DPSGRSAINV VPLFETIEDL QACAKIMDRL LSIPEYRRLV DSRGSVQEVM LGYSDSNKDG
GFVTSGWELY KAEIGLIEIF EHHGVRLRLF HGRGGSVGRG GGPSYDAIVA QPGGAVNGQI
RITEQGEIIT SKYSNVEVGR NNLEILAAAT LEASLLQPKR VAPHRDYLEA MEQLSALAFK
AYRGLVYETD GFVDYFWAST VINEISTLNI GSRPASRKKT RAIEDLRAIP WVFSWAQCRL
MLPGWYGFGS AVSAWVTEHP EKGIAFLQAM YQEWPFFRTL LSNMDMVLSK SSIGIASRYA
ELVEDTAIRD RIFSRIRAEW HSSIDYLLAI MQQDHLLQSN PLLERSIRHR FPYLDPLNHV
QVQLLREHRT HDPDEQVLRG VQLTINGISA GLRNSG
//
