ID CAS6L_PYRFU Reviewed; 241 AA.
AC Q8U3R3;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 27-MAR-2024, entry version 96.
DE RecName: Full=Putative CRISPR-associated endoribonuclease-like protein Cas6;
GN Name=cas6b; Synonyms=cas6-2, cas6-3; OrderedLocusNames=PF0393;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS), RNA-BINDING, AND LACK OF
RP ENDONUCLEASE ACTIVITY.
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=22447673; DOI=10.1002/prot.24061;
RA Park H.M., Shin M., Sun J., Kim G.S., Lee Y.C., Park J.H., Kim B.Y.,
RA Kim J.S.;
RT "Crystal structure of a Cas6 paralogous protein from Pyrococcus furiosus.";
RL Proteins 80:1895-1900(2012).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA), also
CC called psiRNA (prokaryotic silencing) in this organism (Potential).
CC {ECO:0000305}.
CC -!- SUBUNIT: Binds crRNA.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000305}.
CC -!- CAUTION: No in vitro nuclease activity has been observed against crRNA
CC for this protein. {ECO:0000305|PubMed:22447673}.
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DR EMBL; AE009950; AAL80517.1; -; Genomic_DNA.
DR RefSeq; WP_011011506.1; NZ_CP023154.1.
DR PDB; 3UFC; X-ray; 2.03 A; X=1-241.
DR PDBsum; 3UFC; -.
DR AlphaFoldDB; Q8U3R3; -.
DR SMR; Q8U3R3; -.
DR STRING; 186497.PF0393; -.
DR PaxDb; 186497-PF0393; -.
DR GeneID; 41712190; -.
DR KEGG; pfu:PF0393; -.
DR PATRIC; fig|186497.12.peg.408; -.
DR eggNOG; arCOG04342; Archaea.
DR HOGENOM; CLU_089858_1_1_2; -.
DR OrthoDB; 43942at2157; -.
DR PhylomeDB; Q8U3R3; -.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1890; -; 1.
DR Gene3D; 3.30.70.1900; -; 1.
DR InterPro; IPR049435; Cas_Cas6_C.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR NCBIfam; TIGR01877; cas_cas6; 1.
DR PANTHER; PTHR36984; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR PANTHER; PTHR36984:SF1; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR Pfam; PF21350; Cas6_I-A; 1.
DR Pfam; PF01881; Cas_Cas6_C; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiviral defense; Reference proteome; RNA-binding.
FT CHAIN 1..241
FT /note="Putative CRISPR-associated endoribonuclease-like
FT protein Cas6"
FT /id="PRO_0000417971"
FT STRAND 1..13
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 21..35
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 40..44
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 56..63
FT /evidence="ECO:0007829|PDB:3UFC"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 84..96
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 99..102
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 105..114
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 122..130
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 132..140
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 143..149
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 156..172
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 181..195
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 198..211
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 213..222
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 224..226
FT /evidence="ECO:0007829|PDB:3UFC"
FT HELIX 228..230
FT /evidence="ECO:0007829|PDB:3UFC"
FT STRAND 235..237
FT /evidence="ECO:0007829|PDB:3UFC"
SQ SEQUENCE 241 AA; 28046 MW; A2C70BA1BC4BBD00 CRC64;
MRIEIKLLPL QDNPVIPFNY NYELYSQIVE KAGAIEPRIV KLLESPHGYW TFSRIIIRKR
EIIPEKGIKI LSDDISLYIS SSNKEIIKGI VEGIEKSPEF KIGDVGFLVA DIKALKSKEI
KNVNIFSTLS PIVVRTVKFE GDKLKHWDLY PHDELFLDRL RKVMLLRYHE VMGDLPEDKD
FRIELIKFKP TRLIVKDSYI RGSLMVFRYY GSKEIAKFGY ENGFGEKTNL GFGMVKIIEE
Q
//
