ID CCC1_ORYSJ Reviewed; 989 AA.
AC Q6Z0E2; Q0J6F8;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 24-JAN-2024, entry version 92.
DE RecName: Full=Cation-chloride cotransporter 1;
DE Short=OsCCC1;
DE AltName: Full=Potassium-chloride cotransporter 1;
GN Name=CCC1; Synonyms=KCC1; OrderedLocusNames=Os08g0323700, LOC_Os08g23440;
GN ORFNames=OsJ_26882, OSJNBa0078D03.20;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION.
RX PubMed=21369877; DOI=10.1007/s11103-011-9744-6;
RA Kong X.Q., Gao X.H., Sun W., An J., Zhao Y.X., Zhang H.;
RT "Cloning and functional characterization of a cation-chloride cotransporter
RT gene OsCCC1.";
RL Plant Mol. Biol. 75:567-578(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Probable cation/chloride cotransporter that may mediate
CC potassium-chloride cotransport. Involved in plant development and K(+)
CC and Cl(-) homeostasis. May not be involved in sodium-chloride
CC cotransport. {ECO:0000269|PubMed:21369877}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:21369877}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:21369877}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems and leaves with higher
CC expression in root and leaf tips. {ECO:0000269|PubMed:21369877}.
CC -!- INDUCTION: By KCl in roots and leaves. {ECO:0000269|PubMed:21369877}.
CC -!- MISCELLANEOUS: Plants silencing CCC1 have slightly shorter roots and
CC leaves than wild-type, and are more sensitive to KCl treatment during
CC seed germination and plant development.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF23457.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; GU250733; ADB03187.1; -; mRNA.
DR EMBL; AP005493; BAD03666.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF23457.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM000145; EAZ42309.1; -; Genomic_DNA.
DR RefSeq; XP_015650142.1; XM_015794656.1.
DR AlphaFoldDB; Q6Z0E2; -.
DR SMR; Q6Z0E2; -.
DR STRING; 39947.Q6Z0E2; -.
DR GlyCosmos; Q6Z0E2; 3 sites, No reported glycans.
DR iPTMnet; Q6Z0E2; -.
DR PaxDb; 39947-Q6Z0E2; -.
DR EnsemblPlants; Os08t0323700-01; Os08t0323700-01; Os08g0323700.
DR GeneID; 4345272; -.
DR Gramene; Os08t0323700-01; Os08t0323700-01; Os08g0323700.
DR KEGG; osa:4345272; -.
DR eggNOG; KOG2082; Eukaryota.
DR InParanoid; Q6Z0E2; -.
DR OrthoDB; 5490251at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000007752; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q6Z0E2; baseline and differential.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015379; F:potassium:chloride symporter activity; IMP:UniProtKB.
DR GO; GO:0055064; P:chloride ion homeostasis; IMP:UniProtKB.
DR GO; GO:0006821; P:chloride transport; IMP:UniProtKB.
DR GO; GO:0055075; P:potassium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IMP:UniProtKB.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF100; CATION-CHLORIDE COTRANSPORTER 1; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF03522; SLC12; 2.
PE 2: Evidence at transcript level;
KW Glycoprotein; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..989
FT /note="Cation-chloride cotransporter 1"
FT /id="PRO_0000410467"
FT TOPO_DOM 1..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..167
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..280
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 302..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..365
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 387..405
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..441
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..498
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..519
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 520..522
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..543
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 544..551
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 552..572
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 573..578
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..599
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 600..989
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 256
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 261
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 363
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 989 AA; 108454 MW; 2578C300129EAD44 CRC64;
MENGEIEGAA DDGVPVPAPP NGRRYRPVGS SDRAVIQMTS MEPGSSSSTA VAAVSGITPQ
PPRNLTVDPS MQEDHTVSQG DSKLELFGFD SLVNILGLKS MTGEQIQAPS SPRDGEDVAI
TIGRPKETGP KFGTMMGVFV PCLQNILGII YYIRFTWIVG MAGVWQSLVL VSFCGACTFL
TGISLSAIAT NGAMKGGGPY YLIGRALGPE VGVSIGLCFF LGNAVAGSMY VLGAVETFLD
AVPSAGFFKE SVTVVNNTLV NGTATASTAT ISTPSLHDLQ VYGVIVTILL CFIVFGGVKI
INKVAPAFLI PVLFSLLCIY LGVFIAPRHN APKGITGLSI TTFKDNWGSE YQRTNNAGVP
DPNGSIYWDF NALVGLFFPA VTGIMAGSNR SASLKDTQRS IPIGTLSATL TTTAMYLFSV
LLFGALATRE ELLTDRLLTA TVAWPAPAVI YIGIILSTLG AALQSLTGAP RLLAAIANDD
ILPVLNYFKV SEGAEPHSAT LFTAFICICC VVIGNLDLIT PTITMFFLLC YAGVNLSCFL
LDLLDAPSWR PRWKFHHWSL SLVGALLCVV IMFLISWSFT VVSLALASLI YYYVSLKGKA
GDWGDGFKSA YFQLALRSLR SLGANQVHPK NWYPIPLIFC RPWGKLPENV PCHPKLADFA
NCMKKKGRGM SIFVSIIDGD YHELAEDAKT ACRQLDTYIE YKRCEGVAEI IVAPSMSEGF
RSIVQTMGLG NLKPNIIVMR YPEIWRRENL IQIPSTFVSI INDCIIANKA VVIVKGLDEW
PNEYQRQYGT IDLYWIVRDG GLMLLLSQLL LTKETFESCK IQVFCIAEED TDAEELKADV
KKFLYDLRMH AEVIVVTMKS WEPHMESSSS GAPQDDSQEA YTSAQRRIST YLSEMKETAQ
REGHPLMEDG KQVVVNEQKI EKFLYTMFKL NSTILRYSRM AAVVLVSLPP PPLNHPAYFY
MEYMDLLVEN VPRMLIVRGY RRDVVTFFT
//
