ID CCR9_HUMAN Reviewed; 369 AA.
AC P51686; Q4VBM3; Q549E0; Q9UQQ6;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 27-MAR-2024, entry version 191.
DE RecName: Full=C-C chemokine receptor type 9;
DE Short=C-C CKR-9;
DE Short=CC-CKR-9;
DE Short=CCR-9;
DE AltName: Full=G-protein coupled receptor 28;
DE AltName: Full=GPR-9-6;
DE AltName: CD_antigen=CDw199;
GN Name=CCR9; Synonyms=GPR28;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=10229797;
RA Zaballos A., Gutierrez J., Varona R., Ardavin C., Marquez G.;
RT "Identification of the orphan chemokine receptor GPR-9-6 as CCR9, the
RT receptor for the chemokine TECK.";
RL J. Immunol. 162:5671-5675(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP FUNCTION.
RX PubMed=10640743; DOI=10.4049/jimmunol.164.3.1293;
RA Yu C.-R., Peden K.W.C., Zaitseva M.B., Golding H., Farber J.M.;
RT "CCR9A and CCR9B: two receptors for the chemokine CCL25/TECK/Ck beta-15
RT that differ in their sensitivities to ligand.";
RL J. Immunol. 164:1293-1305(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2).
RA Lautens L.L., Tiffany H.L., Gao J.-L., Modi W., Murphy P.M., Bonner T.I.;
RT "Cloning, tissue distribution and chromosomal localization of two potential
RT G-protein-linked chemokine receptors.";
RL Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Warren C.N., Aronstam R.S., Sharma S.V.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 24-341, SUBCELLULAR LOCATION, AND
RP DISULFIDE BOND.
RX PubMed=27926729; DOI=10.1038/nature20606;
RA Oswald C., Rappas M., Kean J., Dore A.S., Errey J.C., Bennett K.,
RA Deflorian F., Christopher J.A., Jazayeri A., Mason J.S., Congreve M.,
RA Cooke R.M., Marshall F.H.;
RT "Intracellular allosteric antagonism of the CCR9 receptor.";
RL Nature 540:462-465(2016).
CC -!- FUNCTION: Receptor for chemokine SCYA25/TECK. Subsequently transduces a
CC signal by increasing the intracellular calcium ions level.
CC {ECO:0000269|PubMed:10229797, ECO:0000269|PubMed:10640743}.
CC -!- FUNCTION: (Microbial infection) Alternative coreceptor with CD4 for
CC HIV-1 infection.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:27926729};
CC Multi-pass membrane protein {ECO:0000305|PubMed:27926729}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=CCR9A;
CC IsoId=P51686-1; Sequence=Displayed;
CC Name=2; Synonyms=CCR9B;
CC IsoId=P51686-2; Sequence=VSP_020286;
CC -!- TISSUE SPECIFICITY: Highly expressed in the thymus and low in lymph
CC nodes and spleen. {ECO:0000269|PubMed:10229797,
CC ECO:0000269|PubMed:10640743}.
CC -!- MISCELLANEOUS: EC50 of SCYA25/TECK for isoform 1 is lower than for
CC isoform 2.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH95516.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Wikipedia; Note=CC chemokine receptors entry;
CC URL="https://en.wikipedia.org/wiki/CC_chemokine_receptors";
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="https://atlasgeneticsoncology.org/gene/44380/CCR9";
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DR EMBL; AJ132337; CAB43477.1; -; mRNA.
DR EMBL; AF145439; AAF66699.1; -; mRNA.
DR EMBL; AF145440; AAF66700.1; -; mRNA.
DR EMBL; U45982; AAA93319.1; -; Genomic_DNA.
DR EMBL; AY242127; AAO92294.1; -; Genomic_DNA.
DR EMBL; BC069678; AAH69678.1; -; mRNA.
DR EMBL; BC095516; AAH95516.1; ALT_INIT; mRNA.
DR CCDS; CCDS2732.1; -. [P51686-1]
DR CCDS; CCDS2733.1; -. [P51686-2]
DR RefSeq; NP_001243298.1; NM_001256369.1. [P51686-2]
DR RefSeq; NP_006632.2; NM_006641.3. [P51686-2]
DR RefSeq; NP_112477.1; NM_031200.2. [P51686-1]
DR RefSeq; XP_011531614.1; XM_011533312.2.
DR PDB; 5LWE; X-ray; 2.80 A; A/B=24-341.
DR PDBsum; 5LWE; -.
DR AlphaFoldDB; P51686; -.
DR SMR; P51686; -.
DR BioGRID; 116017; 18.
DR DIP; DIP-5884N; -.
DR IntAct; P51686; 18.
DR MINT; P51686; -.
DR STRING; 9606.ENSP00000350256; -.
DR BindingDB; P51686; -.
DR ChEMBL; CHEMBL5815; -.
DR DrugBank; DB15250; Vercirnon.
DR GuidetoPHARMACOLOGY; 66; -.
DR GlyCosmos; P51686; 1 site, No reported glycans.
DR GlyGen; P51686; 1 site.
DR iPTMnet; P51686; -.
DR PhosphoSitePlus; P51686; -.
DR BioMuta; CCR9; -.
DR DMDM; 114152781; -.
DR PaxDb; 9606-ENSP00000350256; -.
DR PeptideAtlas; P51686; -.
DR ProteomicsDB; 56370; -. [P51686-1]
DR ProteomicsDB; 56371; -. [P51686-2]
DR Antibodypedia; 3479; 677 antibodies from 36 providers.
DR DNASU; 10803; -.
DR Ensembl; ENST00000357632.7; ENSP00000350256.2; ENSG00000173585.18. [P51686-1]
DR Ensembl; ENST00000395963.2; ENSP00000379292.2; ENSG00000173585.18. [P51686-2]
DR Ensembl; ENST00000706789.1; ENSP00000516552.1; ENSG00000173585.18. [P51686-2]
DR GeneID; 10803; -.
DR KEGG; hsa:10803; -.
DR MANE-Select; ENST00000357632.7; ENSP00000350256.2; NM_031200.3; NP_112477.1.
DR UCSC; uc003coz.3; human. [P51686-1]
DR AGR; HGNC:1610; -.
DR CTD; 10803; -.
DR DisGeNET; 10803; -.
DR GeneCards; CCR9; -.
DR HGNC; HGNC:1610; CCR9.
DR HPA; ENSG00000173585; Tissue enriched (lymphoid).
DR MIM; 604738; gene.
DR neXtProt; NX_P51686; -.
DR OpenTargets; ENSG00000173585; -.
DR PharmGKB; PA26174; -.
DR VEuPathDB; HostDB:ENSG00000173585; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01030000234667; -.
DR HOGENOM; CLU_009579_8_3_1; -.
DR InParanoid; P51686; -.
DR OMA; PYNCVLL; -.
DR OrthoDB; 5357410at2759; -.
DR PhylomeDB; P51686; -.
DR TreeFam; TF330966; -.
DR PathwayCommons; P51686; -.
DR Reactome; R-HSA-380108; Chemokine receptors bind chemokines.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; P51686; -.
DR SIGNOR; P51686; -.
DR BioGRID-ORCS; 10803; 6 hits in 1147 CRISPR screens.
DR GeneWiki; CCR9; -.
DR GenomeRNAi; 10803; -.
DR Pharos; P51686; Tchem.
DR PRO; PR:P51686; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; P51686; Protein.
DR Bgee; ENSG00000173585; Expressed in thymus and 78 other cell types or tissues.
DR ExpressionAtlas; P51686; baseline and differential.
DR Genevisible; P51686; HS.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0019957; F:C-C chemokine binding; IBA:GO_Central.
DR GO; GO:0016493; F:C-C chemokine receptor activity; IBA:GO_Central.
DR GO; GO:0004950; F:chemokine receptor activity; TAS:ProtInc.
DR GO; GO:0019722; P:calcium-mediated signaling; IBA:GO_Central.
DR GO; GO:0002305; P:CD8-positive, gamma-delta intraepithelial T cell differentiation; IEA:Ensembl.
DR GO; GO:0060326; P:cell chemotaxis; IBA:GO_Central.
DR GO; GO:0006968; P:cellular defense response; TAS:ProtInc.
DR GO; GO:0006935; P:chemotaxis; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0006955; P:immune response; IBA:GO_Central.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR CDD; cd15174; 7tmA_CCR9; 1.
DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR InterPro; IPR004069; Chemokine_CCR9.
DR InterPro; IPR000355; Chemokine_rcpt.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR PANTHER; PTHR10489:SF664; C-C CHEMOKINE RECEPTOR TYPE 9; 1.
DR PANTHER; PTHR10489; CELL ADHESION MOLECULE; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00657; CCCHEMOKINER.
DR PRINTS; PR01531; CHEMOKINER9.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Host-virus interaction; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..369
FT /note="C-C chemokine receptor type 9"
FT /id="PRO_0000069291"
FT TOPO_DOM 1..48
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 49..74
FT /note="Helical; Name=1"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 75..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 86..109
FT /note="Helical; Name=2"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 110..120
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 121..150
FT /note="Helical; Name=3"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 151..159
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 160..185
FT /note="Helical; Name=4"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 186..208
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 209..243
FT /note="Helical; Name=5"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 244..248
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 249..283
FT /note="Helical; Name=6"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 284..290
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TRANSMEM 291..321
FT /note="Helical; Name=7"
FT /evidence="ECO:0000269|PubMed:27926729"
FT TOPO_DOM 322..369
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:27926729"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 38..289
FT /evidence="ECO:0000269|PubMed:27926729"
FT DISULFID 119..198
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521,
FT ECO:0000269|PubMed:27926729, ECO:0007744|PDB:5LWE"
FT VAR_SEQ 1..12
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10640743,
FT ECO:0000303|PubMed:15489334, ECO:0000303|Ref.4"
FT /id="VSP_020286"
FT VARIANT 92
FT /note="I -> V (in dbSNP:rs45530037)"
FT /id="VAR_029208"
FT VARIANT 284
FT /note="M -> V (in dbSNP:rs12721497)"
FT /id="VAR_020068"
FT HELIX 33..37
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 45..75
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 82..106
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 117..148
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 155..157
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 158..179
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 181..185
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 209..222
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 224..243
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 249..252
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 254..282
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 289..307
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 308..311
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 313..320
FT /evidence="ECO:0007829|PDB:5LWE"
FT HELIX 324..341
FT /evidence="ECO:0007829|PDB:5LWE"
SQ SEQUENCE 369 AA; 42016 MW; F27CEA0CFB66B44C CRC64;
MTPTDFTSPI PNMADDYGSE STSSMEDYVN FNFTDFYCEK NNVRQFASHF LPPLYWLVFI
VGALGNSLVI LVYWYCTRVK TMTDMFLLNL AIADLLFLVT LPFWAIAAAD QWKFQTFMCK
VVNSMYKMNF YSCVLLIMCI SVDRYIAIAQ AMRAHTWREK RLLYSKMVCF TIWVLAAALC
IPEILYSQIK EESGIAICTM VYPSDESTKL KSAVLTLKVI LGFFLPFVVM ACCYTIIIHT
LIQAKKSSKH KALKVTITVL TVFVLSQFPY NCILLVQTID AYAMFISNCA VSTNIDICFQ
VTQTIAFFHS CLNPVLYVFV GERFRRDLVK TLKNLGCISQ AQWVSFTRRE GSLKLSSMLL
ETTSGALSL
//
