ID CNNM2_CAEEL Reviewed; 760 AA.
AC Q9GYL2;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2023, sequence version 3.
DT 27-MAR-2024, entry version 125.
DE RecName: Full=Metal transporter cnnm-2 {ECO:0000305};
DE AltName: Full=CNNM family homolog 2 {ECO:0000312|WormBase:R04E5.2};
DE Flags: Precursor;
GN Name=cnnm-2 {ECO:0000312|WormBase:R04E5.2};
GN ORFNames=R04E5.2 {ECO:0000312|WormBase:R04E5.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27564576; DOI=10.1371/journal.pgen.1006276;
RA Ishii T., Funato Y., Hashizume O., Yamazaki D., Hirata Y., Nishiwaki K.,
RA Kono N., Arai H., Miki H.;
RT "Mg2+ extrusion from intestinal epithelia by CNNM proteins is essential for
RT gonadogenesis via AMPK-TORC1 signaling in Caenorhabditis elegans.";
RL PLoS Genet. 12:E1006276-E1006276(2016).
CC -!- FUNCTION: Probable metal transporter. Probably acts redundantly with
CC the other metal transport proteins cnnm-1, cnnm-3, cnnm-4 and cnnm-5 to
CC regulate Mg(2+) homeostasis. {ECO:0000305|PubMed:27564576}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Double knockout with cnnm-3
CC results in 22% sterility. Quintuple knockout with cnnm-1, cnnm-3, cnnm-
CC 4 and cnnm-5 results in a reduced lifespan and 100% sterility.
CC {ECO:0000269|PubMed:27564576}.
CC -!- SIMILARITY: Belongs to the ACDP family. {ECO:0000305}.
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DR EMBL; BX284606; CCD65954.2; -; Genomic_DNA.
DR RefSeq; NP_509493.1; NM_077092.3.
DR AlphaFoldDB; Q9GYL2; -.
DR SMR; Q9GYL2; -.
DR STRING; 6239.R04E5.2.1; -.
DR GlyCosmos; Q9GYL2; 6 sites, No reported glycans.
DR EPD; Q9GYL2; -.
DR PaxDb; 6239-R04E5-2; -.
DR PeptideAtlas; Q9GYL2; -.
DR EnsemblMetazoa; R04E5.2.1; R04E5.2.1; WBGene00019869.
DR UCSC; R04E5.2; c. elegans.
DR AGR; WB:WBGene00019869; -.
DR WormBase; R04E5.2; CE53658; WBGene00019869; cnnm-2.
DR eggNOG; KOG2118; Eukaryota.
DR HOGENOM; CLU_011310_1_2_1; -.
DR InParanoid; Q9GYL2; -.
DR OMA; HICYKER; -.
DR PhylomeDB; Q9GYL2; -.
DR PRO; PR:Q9GYL2; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00019869; Expressed in material anatomical entity and 3 other cell types or tissues.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:UniProtKB.
DR GO; GO:0010960; P:magnesium ion homeostasis; IEA:InterPro.
DR GO; GO:0006811; P:monoatomic ion transport; IEA:UniProtKB-KW.
DR GO; GO:1905941; P:positive regulation of gonad development; IGI:UniProtKB.
DR CDD; cd04590; CBS_pair_CorC_HlyC_assoc; 1.
DR Gene3D; 3.10.580.10; CBS-domain; 1.
DR InterPro; IPR045095; ACDP.
DR InterPro; IPR046342; CBS_dom_sf.
DR InterPro; IPR002550; CNNM.
DR InterPro; IPR044751; Ion_transp-like_CBS.
DR PANTHER; PTHR12064; METAL TRANSPORTER CNNM; 1.
DR PANTHER; PTHR12064:SF23; METAL TRANSPORTER CNNM-2; 1.
DR Pfam; PF01595; CNNM; 1.
DR SUPFAM; SSF54631; CBS-domain pair; 1.
DR PROSITE; PS51846; CNNM; 1.
PE 3: Inferred from homology;
KW Cell membrane; Glycoprotein; Ion transport; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..760
FT /note="Metal transporter cnnm-2"
FT /evidence="ECO:0000305"
FT /id="PRO_5004327911"
FT TOPO_DOM 22..153
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 252..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 262..282
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..760
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 145..323
FT /note="CNNM transmembrane"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01193"
FT DOMAIN 344..406
FT /note="CBS 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT DOMAIN 442..512
FT /note="CBS 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT REGION 708..734
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 302
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 528
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 592
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 667
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 760 AA; 85012 MW; F9BDD800544D36F8 CRC64;
MIIKVFLRLL LLCAHIVCID GKLEIRPVVS GVRIESDVDA SGFLGYGDAG ELLVEANTEV
DLVIFGHGLE NVEMVTFTDS VCVTSEFNVS ESTFYIHKDM KIVFKYAFVA WPQPWRICLK
SECHGLIQID DDRTWIQAVQ STHETFMPVW AQCAILCLLF SISALCSGLT LGLMALTPQE
LSILMKSGSQ REKKHAAAIY PIRCHGNRLL CTVIIMNVIV NTGITLLFDD LAEGLIAFVA
STVGIVVFGE ILPQSICVKY GLAVGANTIF ITKFFMFLLF PITWPLGKIL DKYAGVDIDV
VNRSRMVEML KMNMENDACD IDLSTLKIAI GAMELTKKSV RDVMTDIDDV FMLSEDQVLN
AETMTKISDS GYTRIPVFEG NNRNKVKNLL YVSDLALIGK DNNITVKAVA RFNKRRLRIV
DESMPLTALM DEFKLGDYHL AMVAKATEVK KHHHGKFADG TVDSFILKSM KLVEATMMPQ
VENPEDHPVT LVGLITLEDI TEELLQAEIT DETDCYVTDD AQKKRRTNTS KKSAAELFCS
EKKSERLSLH MLEMTEKWLL EKTPLFGNMN PKAFENLIQR NIREVLIVPP KNSTSPGTLN
LFEAGVMSKR FLLILEGKAT IRFNEKDLIF ECGPWTCFGE AILEKMEMCI SDRKEPSTGF
FFLPDYNLTV SGPCRFLQIS TSSLLHSLRI TQFVKEIRTP KISITSDDDF GSPTRKASIL
DSSPNSRKRS STSVMNSLAL PTARLAAKIA SVEELKPLME
//
