ID COG8_YEAST Reviewed; 607 AA.
AC Q04632; D6VZA2; Q03641;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 27-MAR-2024, entry version 168.
DE RecName: Full=Conserved oligomeric Golgi complex subunit 8;
DE Short=COG complex subunit 8;
DE AltName: Full=Component of oligomeric Golgi complex 8;
GN Name=COG8; Synonyms=DOR1; OrderedLocusNames=YML071C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=11703943; DOI=10.1016/s1534-5807(01)00063-6;
RA Whyte J.R., Munro S.;
RT "The Sec34/35 Golgi transport complex is related to the exocyst, defining a
RT family of complexes involved in multiple steps of membrane traffic.";
RL Dev. Cell 1:527-537(2001).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP COMPOSITION OF THE COG COMPLEX, AND INTERACTION WITH COG3 AND COG4.
RX PubMed=15047703; DOI=10.1074/jbc.m400662200;
RA Loh E., Hong W.;
RT "The binary interacting network of the conserved oligomeric Golgi tethering
RT complex.";
RL J. Biol. Chem. 279:24640-24648(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Acts as a component of the peripheral membrane COG complex
CC that is involved in intra-Golgi protein trafficking. COG is located at
CC the cis-Golgi, and regulates tethering of retrograde intra-Golgi
CC vesicles and possibly a number of other membrane trafficking events.
CC {ECO:0000269|PubMed:11703943}.
CC -!- SUBUNIT: Component of the conserved oligomeric Golgi (COG or
CC Sec34/Sec35) complex which consists of eight different proteins COG1-
CC COG8. {ECO:0000269|PubMed:11703943}.
CC -!- INTERACTION:
CC Q04632; P53079: COG1; NbExp=8; IntAct=EBI-6035, EBI-4835;
CC Q04632; P53271: COG2; NbExp=4; IntAct=EBI-6035, EBI-16614;
CC Q04632; P53951: COG5; NbExp=3; IntAct=EBI-6035, EBI-4841;
CC Q04632; P53959: COG6; NbExp=5; IntAct=EBI-6035, EBI-4829;
CC Q04632; P53195: COG7; NbExp=2; IntAct=EBI-6035, EBI-4847;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 6440 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the COG8 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z38114; CAA86249.1; -; Genomic_DNA.
DR EMBL; Z46373; CAA86507.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09826.1; -; Genomic_DNA.
DR PIR; S48326; S48326.
DR RefSeq; NP_013640.1; NM_001182430.1.
DR AlphaFoldDB; Q04632; -.
DR BioGRID; 35070; 406.
DR ComplexPortal; CPX-1840; COG Golgi transport complex.
DR DIP; DIP-6749N; -.
DR IntAct; Q04632; 21.
DR MINT; Q04632; -.
DR STRING; 4932.YML071C; -.
DR iPTMnet; Q04632; -.
DR MaxQB; Q04632; -.
DR PaxDb; 4932-YML071C; -.
DR PeptideAtlas; Q04632; -.
DR EnsemblFungi; YML071C_mRNA; YML071C; YML071C.
DR GeneID; 854904; -.
DR KEGG; sce:YML071C; -.
DR AGR; SGD:S000004536; -.
DR SGD; S000004536; COG8.
DR VEuPathDB; FungiDB:YML071C; -.
DR eggNOG; KOG2069; Eukaryota.
DR GeneTree; ENSGT00390000015893; -.
DR HOGENOM; CLU_031416_0_0_1; -.
DR InParanoid; Q04632; -.
DR OMA; CLQLVYC; -.
DR OrthoDB; 1442148at2759; -.
DR BioCyc; YEAST:G3O-32665-MONOMER; -.
DR BioGRID-ORCS; 854904; 2 hits in 10 CRISPR screens.
DR PRO; PR:Q04632; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04632; Protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; NAS:ComplexPortal.
DR GO; GO:0017119; C:Golgi transport complex; IMP:SGD.
DR GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IMP:SGD.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0000301; P:retrograde transport, vesicle recycling within Golgi; NAS:ComplexPortal.
DR InterPro; IPR007255; COG8.
DR PANTHER; PTHR21311; CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 8; 1.
DR PANTHER; PTHR21311:SF0; CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 8; 1.
DR Pfam; PF04124; Dor1; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..607
FT /note="Conserved oligomeric Golgi complex subunit 8"
FT /id="PRO_0000213525"
FT REGION 375..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 410
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 607 AA; 69829 MW; 57E34E2DE36664B6 CRC64;
MELILNSLIS DDLTEEQKRL SLDFLQDILQ SNTKDYESYF SSRAVPGSIT EDIAEIDAEL
SALDRKIRKT LLDNTSQIIG NILENDDRAQ LDDIAKSLEQ LWELDTNINK AADRNVTNDD
INNESVSIDD FLEDDKEDND TGRIMTTESN NLARKKKEDE FHKALSRLRN RISTKEDDKD
DIRSDTLVTV LENLDSITDL MELPFLARTC IRTGHYQEAV MLYTHTTSLR SRFPGSTIVD
EVCEKVLNEI STTMLSGLVK LLSTNVSVNS LKKILQYLNS IPPFDGKTNK SLLSVFLAMR
YKFITDEIAS YPLDVESSNE SLIEMMVKRK IEVLREHVYM SLNVFLKSFL YDTNDLEIPF
PEELESTVLR INGTNEEKEI EEKEKETKKE EYQKQDSVAN NEEDVTENKS IEDVQEEVQG
KVEGEDDGAE RKTENEIENE TVNKTEDKAE KEKEEEVNTK DNKAEKEEEE INKVEVTPEE
PSKSIDNKAE KEEEEINKVE VTPEEPSKKI RTSKRENKIP TNAVMLQFVD KCITYVLKDL
TRGLNSIKLS DSVCLQLVYC SFRLCDLNRN YHHLFLKKIN DTSLFTTEQL ARAIDKRAEL
ASKYIYS
//
