ID D0BKK8_9LACT Unreviewed; 1441 AA.
AC D0BKK8;
DT 24-NOV-2009, integrated into UniProtKB/TrEMBL.
DT 24-NOV-2009, sequence version 1.
DT 24-JAN-2024, entry version 83.
DE RecName: Full=DNA polymerase III PolC-type {ECO:0000256|HAMAP-Rule:MF_00356};
DE Short=PolIII {ECO:0000256|HAMAP-Rule:MF_00356};
DE EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_00356};
GN Name=polC {ECO:0000256|HAMAP-Rule:MF_00356};
GN ORFNames=HMPREF0446_00493 {ECO:0000313|EMBL:EEW93611.1};
OS Granulicatella elegans ATCC 700633.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
OC Granulicatella.
OX NCBI_TaxID=626369 {ECO:0000313|EMBL:EEW93611.1, ECO:0000313|Proteomes:UP000002939};
RN [1] {ECO:0000313|EMBL:EEW93611.1, ECO:0000313|Proteomes:UP000002939}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700633 {ECO:0000313|EMBL:EEW93611.1,
RC ECO:0000313|Proteomes:UP000002939};
RG The Broad Institute Genome Sequencing Platform;
RA Ward D., Feldgarden M., Earl A., Young S.K., Zeng Q., Koehrsen M.,
RA Alvarado L., Berlin A., Bochicchio J., Borenstein D., Chapman S.B.,
RA Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D., Larson L.,
RA Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T.,
RA Shenoy N., Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J.,
RA Yandava C., Sibley C.D., Field T.R., Grinwis M., Eshaghurshan C.S.,
RA Surette M.G., Haas B., Nusbaum C., Birren B.;
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EEW93611.1, ECO:0000313|Proteomes:UP000002939}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700633 {ECO:0000313|EMBL:EEW93611.1,
RC ECO:0000313|Proteomes:UP000002939};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A., Ward D., Feldgarden M., Gevers D., Sibley C.D., Field T.R.,
RA Grinwis M., Eshaghurshan C.S., Surette M.G., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Brown A., Chapman S.B., Chen Z., Dunbar C.,
RA Freedman E., Gearin G., Goldberg J., Griggs A., Gujja S., Heiman D.,
RA Howarth C., Larson L., Lui A., MacDonald P.J.P., Montmayeur A., Murphy C.,
RA Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Granulicatella elegans ATCC 700633.";
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Required for replicative DNA synthesis. This DNA polymerase
CC also exhibits 3' to 5' exonuclease activity.
CC {ECO:0000256|ARBA:ARBA00003452, ECO:0000256|HAMAP-Rule:MF_00356}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_00356};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00356}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. PolC
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_00356}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EEW93611.1}.
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DR EMBL; ACRF02000013; EEW93611.1; -; Genomic_DNA.
DR RefSeq; WP_006702767.1; NZ_KI391971.1.
DR STRING; 626369.HMPREF0446_00493; -.
DR eggNOG; COG2176; Bacteria.
DR HOGENOM; CLU_003297_2_0_9; -.
DR OrthoDB; 9804290at2; -.
DR Proteomes; UP000002939; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd07435; PHP_PolIIIA_POLC; 1.
DR CDD; cd04484; polC_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.30.1900.20; -; 2.
DR Gene3D; 6.10.140.1510; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 1.10.150.700; PolC, middle finger domain; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_00356; DNApol_PolC; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR024754; DNA_PolC-like_N_II.
DR InterPro; IPR028112; DNA_PolC-type_N_I.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR006054; DnaQ.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR006308; Pol_III_a_PolC-type_gram_pos.
DR InterPro; IPR044923; PolC_middle_finger_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00573; dnaq; 1.
DR NCBIfam; TIGR01405; polC_Gram_pos; 1.
DR PANTHER; PTHR32294:SF5; DNA POLYMERASE III POLC-TYPE; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF14480; DNA_pol3_a_NI; 1.
DR Pfam; PF11490; DNA_pol3_a_NII; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 2.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF160975; AF1531-like; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00356};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_00356};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_00356};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_00356};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00356};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00356};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00356}; Reference proteome {ECO:0000313|Proteomes:UP000002939};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00356}.
FT DOMAIN 340..407
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT DOMAIN 425..591
FT /note="Exonuclease"
FT /evidence="ECO:0000259|SMART:SM00479"
SQ SEQUENCE 1441 AA; 163689 MW; 7912BEC55E9908B7 CRC64;
MSEHQQQLFL TLMNQLQLDN EQFLQPDFDQ ASLTKIDITK SIPEWCFNIE TNHVIQYEKF
KRFYEQLKQT YEKIAQVRLV VKTREESQIT QEQIQAYWEM VLFQLQQESP LVVQLLSGQI
PTWNQKKIQF HCPNEITKSH VNENYIKKIQ QYFENFGFPK MGVDIIVNDE LGSQLEEELR
IKHETIEQEY QEKAVQQKEV QETAASKPAA VNSDSSILYG KAIRSNLETR EIADIYEEER
NVLVEAQIFD IEIRTRKASG KSFANIKMTD YTSSIGATLF PSTAEDEERL AALKKGTWVK
AYGNIEINKF SQELNMIVRD MNKVEHIGRI DRAPEDQKRI ELHLHTNMSV MDATNSASDL
VKQAAKWGHP AVAITDHANL QSYPEAHGAG KGNNIKILYG LEGNIVDDQV NVAYNPQHIL
LEDATYVVFD VETTGLSAIY DSIIELAAVK MHKGNVIDKF EEFIDPGHPL SATTIQLTGI
TDDMVRGSKS QAQVLKEFAA FTKDTILVAH NASFDMGFLN TGYEKEGLEK TTQPVIDTLE
LSRLLHPHLK SHRLNTLAKR YNVALEQHHR AVFDSETTGH LCHIFLKEAA NDHGLIYHDQ
LNTNLHPEEV FKNGRPFHAT IFAKHQAGLK ELFKVVSASN VQYFYRVPRI LRSMLIENRD
SFLIGSGCSE GEVFEAMMQK GYEEAKEKAK FYDYIEIMPK AVYRPLIDKE LIRNEHHLEE
IIKNLVKLGE ELDIPVVATG NVHYLNPEDK IYREILLTSV NNGQKLNYPD VHFRTTNEML
DEFAFLGEEK AFEVVVTNTH WVNDQLEVII PIKDDLYTPK IEGAEDEITK LSYDKAHEWY
GEELPEIVEK RIDKELKSII GNGFSVIYLI SQKLVHKSNE DGYLVGSRGS VGSSLVATLT
GITEVNPLAP HYRCPNCCYS EFFDDGSVGS GFDLPEKDCP KCGTRLAKDG HDIPFETFLG
FYGDKVPDID LNFSGEYQPQ AHNYTKVLFG EDYVYRAGTI GTVADKTAFG YVKGYERDLG
LEFRQAEIDR LAKGCTGVKR STGQHPGGII VIPDYMDVYD FTPIQFPADD QNAEWKTTHF
DFHSIHDNVL KLDILGHDDP TVIRMLQDLS GIEPKDIPPD DPEVMKIFGG TEVLGVTPEQ
IDSNTGTLGI PEFGTKFVRG MLEQTKPTTF TELLQISGLS HGTDVYLGNA EELIRLHGIP
LSEVIGCRDD IMVYLIHKGV ENGAAFKIME GVRKGKGIPE DYQAMMRENN VPEWYIESCL
KIKYMFPKAH AAAYVLMALR VAWFKVHKPL LYYCAYFSVR AQDFDIVAMS QGKEMIKQRM
KEIRDKGNDA STKEVNLLTV LELANEMCER GFNFKMVDIE KSDAVNFVIE GDSLIAPFRS
IPGLGLNVAN KIVEARQEQP FISKKDLATR GKVSKTLIDY MTENKVLDDL PDENQLSLFD
F
//
