ID D0LN44_HALO1 Unreviewed; 3045 AA.
AC D0LN44;
DT 15-DEC-2009, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2009, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE SubName: Full=KR domain protein {ECO:0000313|EMBL:ACY13415.1};
DE SubName: Full=Polyketide synthase {ECO:0000313|EMBL:ALX87678.1};
GN Name=hlaB {ECO:0000313|EMBL:ALX87678.1};
GN OrderedLocusNames=Hoch_0799 {ECO:0000313|EMBL:ACY13415.1};
OS Haliangium ochraceum (strain DSM 14365 / JCM 11303 / SMP-2).
OC Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae; Haliangium.
OX NCBI_TaxID=502025 {ECO:0000313|EMBL:ACY13415.1, ECO:0000313|Proteomes:UP000001880};
RN [1] {ECO:0000313|EMBL:ACY13415.1, ECO:0000313|Proteomes:UP000001880}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14365 {ECO:0000313|EMBL:ACY13415.1}, and DSM 14365 / CIP
RC 107738 / JCM 11303 / AJ 13395 / SMP-2
RC {ECO:0000313|Proteomes:UP000001880};
RX PubMed=21304682; DOI=10.4056/sigs.69.1277;
RG US DOE Joint Genome Institute (JGI-PGF);
RA Ivanova N., Daum C., Lang E., Abt B., Kopitz M., Saunders E., Lapidus A.,
RA Lucas S., Glavina Del Rio T., Nolan M., Tice H., Copeland A., Cheng J.F.,
RA Chen F., Bruce D., Goodwin L., Pitluck S., Mavromatis K., Pati A.,
RA Mikhailova N., Chen A., Palaniappan K., Land M., Hauser L., Chang Y.J.,
RA Jeffries C.D., Detter J.C., Brettin T., Rohde M., Goker M., Bristow J.,
RA Markowitz V., Eisen J.A., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Haliangium ochraceum type strain (SMP-2).";
RL Stand. Genomic Sci. 2:96-106(2010).
RN [2] {ECO:0000313|EMBL:ALX87678.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=SMP-2 {ECO:0000313|EMBL:ALX87678.1};
RX PubMed=26751435;
RA Sun Y., Tomura T., Sato J., Iizuka T., Fudou R., Ojika M.;
RT "Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid
RT Metabolite from the Marine Myxobacterium Haliangium ochraceum.";
RL Molecules 21:E59-E59(2016).
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DR EMBL; CP001804; ACY13415.1; -; Genomic_DNA.
DR EMBL; KU516824; ALX87678.1; -; Genomic_DNA.
DR STRING; 502025.Hoch_0799; -.
DR KEGG; hoh:Hoch_0799; -.
DR eggNOG; COG0604; Bacteria.
DR eggNOG; COG2267; Bacteria.
DR eggNOG; COG3321; Bacteria.
DR eggNOG; COG4424; Bacteria.
DR HOGENOM; CLU_225957_0_0_7; -.
DR OrthoDB; 3337911at2; -.
DR Proteomes; UP000001880; Chromosome.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd05195; enoyl_red; 1.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR013149; ADH-like_C.
DR InterPro; IPR013154; ADH-like_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00561; Abhydrolase_1; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF08240; ADH_N; 1.
DR Pfam; PF00107; ADH_zinc_N; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF13469; Sulfotransfer_3; 1.
DR PRINTS; PR00111; ABHYDROLASE.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SMART; SM00822; PKS_KR; 1.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF50129; GroES-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 3.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000001880};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 29..445
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1701..1777
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1791..2206
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2360..2437
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 2445..2479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3045 AA; 322400 MW; E4DFA1A7D8988A6F CRC64;
MPHAERLRRA VLLIKKMEAQ LAAERASRRA PIAIIGSACR LPGGVADRDA LWKVLRDGTE
TAEAVPAARW QMAANTHAGA RWGAFIDDVD CFDPSLFGVS PREAAQADPQ QRLALELVWA
ALEDAGMAPD GLHGTRAGVW LGMSGGDYSS MLSWSRPTGL DLHGMTGAIA AFLPGRLSYA
LGLQGPSMTV DTACSSSLVA VHLACNSLRA GETSLALVGG INLMLWGMHN DGLAGSGALS
PDGKCYTFDS RANGYVRGEG GGVFVLKRLE DAQRDDDRIL ATILGSAVNH NGRSTGLTTP
NGLAQQDLLR DALADAGLSA GDLDVLEVHG TGTPLGDSIE VDAIRAALGT GGERPLWMGS
VKANLGHLEA AAGVPSLLKL ILALRHEQLP PQATFRSLNP LIDLADSRLR IAERAVPWPS
GERPRRAGMS GFGMSGTNAH LIIGEAPARA EPAAGPERPV HVLPLATTDQ PAMARRIAQM
SAWIHSHPEA RAGDVAAAAA RRMARLPVRA AVVAADLPGL EAGLERLAAG TSGPGAVRGA
AIEMRPRIGW LFTGQGAQRA DMGRGLYEHH PGFRETLDRC ADALGRAHDL REVMWSSDGR
LDRTGWTQPA LFALEVSLAA LWRQWGIEPE VLVGHSVGEI AAACVAGVFS IEDGMRLVEA
RARLMDALPE GGAMVAVRGQ PARIERAVAS AEGVSVAAFN GPDQVVISGA SDAVQALASE
LAEAGLRAKA LTVSHAFHSE LMEPMLEDFR AALRDIRFHP PELPLVSNLR GELAGPEVAS
ADYWVEHVRA PVRFLEGMRA AHAVGVDHYL EIGPQPVLCR LGATCVPAGG GETWLPSLQR
TRDDWEVIAN TVAQLHSLGA DIDFAAFDEP FARRWVDLPC YPFERRRFPL PAIAEPDVSE
LSELSDLGDA AFVRGTERMA SPRQPVRAER SSGLVHVVHW TAVADLPESQ PAGQWVVLHE
GAPLAGELAS ALGAVTVELG HSIPACDGLI ILLGRDGEPA DEAARQTRAA LDILAALRDR
RPAPRTVWIT RGASAPGREP SGLAGAALAG MLRCLALEQP ELSPRWIDLA GAGDEGELVA
CLIRELGASE PEVRLWGTRR QLPRLDALSR LPGVLEAPAG PHLRLVRGQG NSLDALSIAA
WQPGPLAPDE VRVRVRAAAL NFRDVLSVLG MYPDDIGEPG GEVVGEVEAL GSEVVGLSVG
DRVMGGGQGG LADRVDIPAM QLVQVPDTLS DVEAATLPIA GGTALFALAE LARVQPGQRV
LIHAGAGGVG MAAIHIARML GAEVLATASP GKHALLRELG VELIANSRSP DFGERLRAAS
GGRGVHVVLN SLTGRFIDEG LSLLEPGGVF VELGKRDVRD PAAIAAGWPG VRYEVLDLTA
LPIEEFHANS QRVADIAARG DLPALRRSLY SLQRAPEALR RLGAGETVGK VVVRPPSANP
VFPTACPVLI TGGRGALGRA VARWLVQHGV RHLVLAGRSE PGQDDLEFAA ALGEQVEQVV
LDVCDEHALA ELLARLGPLG GVVHAAGVLD DAMLHNTDAE RAARVMAPKV QGAWNLHRQT
LNRPPDLFVV FSSVAGVFGN PGQSAYAAGN AFLDALMAWR AEMGLPGTSV AWGAWEGEGM
AAGIDRTRIE AAGLRMLQPE AALQALAEAL ASGLPRTVAT PLPTGRMGKG APALLAHLGE
VRGPAAATGA AAAASSQVAN LGPDALREQL ARRVANLLGH SGLVSIRRPL HELGLDSLMA
VELRNGLAAW TGLELPATLA FDYPSVDAIA SLIEGSLEPV QAAEEAPTRT DEPIAIVGLG
CRFPGADNPQ ALWQIVLKQQ VMVRPVPAER WPADAWPAPE EPGGLPRAAA FLDRVDQLDA
GFFDLSAGEA RDMDPQQRLL LEVGWEALED AGIPPLSLEG SNTGTYVGIC CSDFRELCGD
SALGVHGGTG TLHSVASGRL AYVLGLNGPA ITVDTACSSS LLAVHLACQA LRSGEVDLAL
AGGANVILSP RSSVEINRLS ALSPQGRSLS FSADANGYGR GEGCGVVVLK RLSDAVRAGD
RIVSLILGDA SNHDGRSNGL TAPHSGAQKK VIQQALRRAG VAGREVELIE AHGTGTPLGD
PIEVQALDAV LREGRERPVV LGSLKAGLGH AEAAAGIAGL LKVALAIQAG VLPAQPPVGA
LNPHVAWDRL KVDVIEQPQP WPSERRIAGV SSFGLSGTNV HVVLGAPPPV ETSPTPLQGV
LHLPLSARSA PALRELARAW AERLEQSSLA DLLHTARVGR SHLEHRVCVC GHDAEQLAAA
LRLFAESGTL EPDELARRFM AGEQVEWPLI PGARIVSAPT MRWQRRRYWP RGLDSASLVP
GGASARGETA PDGVNVTDVA DIADVGAWLS EHLVELLNLE PEAVTRDAEL AALGLDSMLS
LELGEAIRDE LELTVYPREL AEIRTLAELE TLLGRLADER VSLQARPAAA PHDAEPELGA
PLEPELESPL GPEGERLRGA PLREGPVFVL SAPRSGSTLL RVMLAGHSRL FAPPELHLLV
AADLAAWRDS PRHLDEGLLE ALVQLGQGTP EDVRALIDQW VAEGLSIADT YRRLMDLCAP
LALVDKSPSS VMDRDALMRV AREFPDARFV WLVRHPLAVV ESMIRRRIHA VVGAVEDPQT
FAEQTWCQSV DNALALRDEV GAERFVTLRY EALVRDPAAA MAHLCDALGL AYEDALLRPY
EGERMTDGLH DGSLSIGDPG FKERRDIEPT LADAWREVRL PRPPSAALCE RAQRLGYPVA
SQRELVSDLV LSTWGPESGD AVVCIHGHLD QGPLWTPVAD RLAAQGLRVL APDLRGHGRS
PHGSLGLFEH LADLDALLAA QAPGRIVLVG HSLGALIAAF YAAARPERVA KLVLLDPGLP
SPLSEGPGAA LARALDRRRD AAHAPMAGLD EAARRLRRAI PDLSEAWSRE LAERVSEQRG
EHRVWRWDPR LRVLSGEGFD RDTALEILAS QHAPVTVAFA ARGDRARPED RRAIEDALGS
ATFVELDTAS HHLHLARTED VVGLIVERAA AQSTMSSPDR STNAP
//
