ID D0NB97_PHYIT Unreviewed; 1645 AA.
AC D0NB97;
DT 15-DEC-2009, integrated into UniProtKB/TrEMBL.
DT 15-DEC-2009, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=PITG_09260 {ECO:0000313|EMBL:EEY55326.1};
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677 {ECO:0000313|EMBL:EEY55326.1, ECO:0000313|Proteomes:UP000006643};
RN [1] {ECO:0000313|Proteomes:UP000006643}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4 {ECO:0000313|Proteomes:UP000006643};
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the ubiquitin conjugation factor E4 family.
CC {ECO:0000256|ARBA:ARBA00007434}.
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DR EMBL; DS028131; EEY55326.1; -; Genomic_DNA.
DR RefSeq; XP_002903550.1; XM_002903504.1.
DR STRING; 403677.D0NB97; -.
DR EnsemblProtists; PITG_09260T0; PITG_09260T0; PITG_09260.
DR GeneID; 9461744; -.
DR KEGG; pif:PITG_09260; -.
DR VEuPathDB; FungiDB:PITG_09260; -.
DR eggNOG; KOG2042; Eukaryota.
DR HOGENOM; CLU_244966_0_0_1; -.
DR InParanoid; D0NB97; -.
DR OMA; CFTRIPN; -.
DR OrthoDB; 145583at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000151; C:ubiquitin ligase complex; IEA:InterPro.
DR GO; GO:0034450; F:ubiquitin-ubiquitin ligase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0030433; P:ubiquitin-dependent ERAD pathway; IEA:InterPro.
DR CDD; cd19757; Bbox1; 1.
DR Gene3D; 3.30.60.90; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR046349; C1-like_sf.
DR InterPro; IPR019474; Ub_conjug_fac_E4_core.
DR InterPro; IPR045132; UBE4.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR000433; Znf_ZZ.
DR InterPro; IPR043145; Znf_ZZ_sf.
DR PANTHER; PTHR13931:SF2; UBIQUITIN CONJUGATION FACTOR E4 B; 1.
DR PANTHER; PTHR13931; UBIQUITINATION FACTOR E4; 1.
DR Pfam; PF04564; U-box; 1.
DR Pfam; PF10408; Ufd2P_core; 2.
DR Pfam; PF00569; ZZ; 1.
DR SMART; SM00504; Ubox; 1.
DR SMART; SM00291; ZnF_ZZ; 2.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57889; Cysteine-rich domain; 1.
DR SUPFAM; SSF57850; RING/U-box; 2.
DR PROSITE; PS51698; U_BOX; 1.
DR PROSITE; PS50135; ZF_ZZ_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000006643};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00228}.
FT DOMAIN 632..700
FT /note="ZZ-type"
FT /evidence="ECO:0000259|PROSITE:PS50135"
FT DOMAIN 1520..1600
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REGION 882..901
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1626..1645
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1645 AA; 184180 MW; 69F72CC0E43586A2 CRC64;
MFVCARAQGG SEDVHALPDS LNASLSTSQR ASRDSSLSVA IDDASSYLQL LGALTRFVAQ
LLDVHTLSPS LRFSAGESVA VDAAEWWKEV QRQSIALSQR QKIAQEAHRD TEEPTARRLW
LLSPRTREKS PVRESRVINN NLWAGEAFKD LPEHVPKANF FMQALYLIMR ALERATRRQQ
GRIEEDSVLG ETLVDALASV DAALAFAAAE LLQLWAPVSH KRVVDSAEKL SKLMNVAVVN
ALLQPRNVGT KHTFHGHILL PSMPLRAFLG AKQWACSVCE RISQTTGSDA PCVYRCAACN
FNLCRECFTR IPNVTNPPAS SPLEELTKLT SDRFFSLVVP QLKHETGKVV VRQITQELRR
RVLELYPHNF QGAAEFAQLF LRLAASDKSA AIALVTSPPA SVPSSGRATC SWFPLFMRTW
TMSLSTLLGP FLRASTMLDE THDLDAAVRA ANHGSVVADC LRAQWTSGYA ELRETLQNIM
QTLLAEGQDP IVVDATLCWL ALALMTADPR RRLNYDAHER LDGFLVNVTT MLLAHALPLL
DKAQHDPSRI DASYHQSVEP VRVYASKDFP EVPLHYSRSQ DTHNENAAGV ETCEIKARDE
DAIAAYNKRR QQEHEEGERA VPSTYYPRLS CHEGVVCDQC EFPNLHGVRY KCAFCGDMDL
CSDCFEIFRS HQAAPDSESE SESILHSPDH VFLRIESPIP VFALKHFQSL PVECLTSKHK
GESCSVLDEG FEGSRIHCAD CGDALGASGQ PDEVFYKCAN CLSSRVVCAA CLAREERLSE
GNPNDMHAPG HVYFVITDPW RRHLSVPWDY VLGKLHFRRL LHPPALIPRR RFPRDTEMFY
ITLKYLHFGP LATLSRWLGM TKELRELQAF CACDEERFEL ERHQRSSRNG SQRQTLKPSV
HYKASKSRLE DIRVKCVKVE LHLLASANVA GWLIFYARTC RWLLYSASAA PETGKAPQDP
FSEPISHFSS MFSGFPEHFF FDLCDVVHLL GLERLEYRDL VSELKRTRPP CEAVDEVTEV
VEPLLVMLTQ IVVAPKFTKN PHLRIEALRS LTTLVTFVSK GQQVHHRPGY QRIESLFKSH
RLLSQCLTPG LLQFHADMDR YNASNNGLAF TSAVSSGDHM LWGFLPTRLS VTMLLRYLWQ
VPSARQSLLH MLSTSETRHT SVSAEPSTDS TQQLTELVSG LWSDIAKLFD EANNKITTLR
QIHDLLEDSL DGSVVVLPFR RDMLDGYVAI NAKHLRLTMR LLLEALESTS WFASIVSGER
SFRSRSLSAP AAALRHVLLQ PLVVEQAART LSFLVASLAS AHEENEWKFS LPLVDDGRLL
LAHLIVLVVR YAGHSAVKSG ASSGACSPSS FWKMIRSGGD IMAKRVGDLD THVRWGFNAL
CTRMESESYL GTSLDDDVED IRVETQAGHE LDEDEALALL EKQAVSSTVS VVDTAAKKRL
SKRFIATLAK DGRFDFRLFV GGCRFLRPQR GSSDNSDERE DVDEGYLYLD DAWVQQFVRA
MQEADEMIHV QEAMEACLGD IPDQYLDPLL STLMTDPVRL PSGNIVDRAV IARHLLASSQ
QGGSTGRDPF TREPLTMAMV EPCDALRSEI QMYLRTKMRH FRQTAREDVL ATWGLGWDVL
FDSSSETEAD AEDGVDGNNA TLRSQ
//
