ID D1LNU0_I63A3 Unreviewed; 230 AA.
AC D1LNU0;
DT 19-JAN-2010, integrated into UniProtKB/TrEMBL.
DT 19-JAN-2010, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=Non-structural protein 1 {ECO:0000256|RuleBase:RU362113};
DE Short=NS1 {ECO:0000256|RuleBase:RU362113};
GN Name=NS1 {ECO:0000313|EMBL:ACZ47281.1};
GN Synonyms=NS {ECO:0000256|RuleBase:RU362113};
OS Influenza A virus (strain A/Duck/Ukraine/1/1963 H3N8).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=385580 {ECO:0000313|EMBL:ACZ47281.1, ECO:0000313|Proteomes:UP000129778};
OH NCBI_TaxID=8782; Aves (birds).
OH NCBI_TaxID=9796; Equus caballus (Horse).
RN [1] {ECO:0000313|EMBL:ACZ47281.1, ECO:0000313|Proteomes:UP000129778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A/duck/Ukraine/1/1963 {ECO:0000313|EMBL:ACZ47281.1};
RA Kim L.M., Scott M.A., Suarez D.L., Spackman E., Swayne D.E., Afonso C.L.;
RT "USDA Agriculture Research Service Avian Influenza Virus Sequencing
RT Project.";
RL Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000129778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Kim L.M., Scott M.A., Suarez D.L., Spackman E., Swayne D.E., Afonso C.L.;
RT "USDA Agriculture Research Service Avian Influenza Virus Sequencing
RT Project.";
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Inhibits post-transcriptional processing of cellular pre-
CC mRNA, by binding and inhibiting two cellular proteins that are required
CC for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage
CC and polyadenylation specificity factor/CPSF4 and the poly(A)-binding
CC protein 2/PABPN1. In turn, unprocessed 3' end pre-mRNAs accumulate in
CC the host nucleus and are no longer exported to the cytoplasm. Cellular
CC protein synthesis is thereby shut off very early after virus infection.
CC Viral protein synthesis is not affected by the inhibition of the
CC cellular 3' end processing machinery because the poly(A) tails of viral
CC mRNAs are produced by the viral polymerase through a stuttering
CC mechanism. Prevents the establishment of the cellular antiviral state
CC by inhibiting TRIM25-mediated RIGI ubiquitination, which normally
CC triggers the antiviral transduction signal that leads to the activation
CC of type I IFN genes by transcription factors IRF3 and IRF7. Also binds
CC poly(A) and U6 snRNA. Inhibits the integrated stress response (ISR) in
CC the infected cell by blocking dsRNA binding by EIF2AK2/PKR and further
CC phosphorylation of EIF2S1/EIF-2ALPHA. Stress granule formation is thus
CC inhibited, which allows protein synthesis and viral replication.
CC {ECO:0000256|RuleBase:RU362113}.
CC -!- SUBUNIT: Homodimer. Interacts with host TRIM25 (via coiled coil); this
CC interaction specifically inhibits TRIM25 multimerization and TRIM25-
CC mediated RIGI CARD ubiquitination. Interacts with human EIF2AK2/PKR,
CC CPSF4, IVNS1ABP and PABPN1. {ECO:0000256|RuleBase:RU362113}.
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000256|RuleBase:RU362113}.
CC Host cytoplasm {ECO:0000256|RuleBase:RU362113}.
CC -!- DOMAIN: The dsRNA-binding region is required for suppression of RNA
CC silencing. {ECO:0000256|RuleBase:RU362113}.
CC -!- SIMILARITY: Belongs to the influenza A viruses NS1 family.
CC {ECO:0000256|RuleBase:RU362113}.
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DR EMBL; GU052325; ACZ47281.1; -; Viral_cRNA.
DR Proteomes; UP000129778; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030291; F:protein serine/threonine kinase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0039524; P:suppression by virus of host mRNA processing; IEA:UniProtKB-UniRule.
DR GO; GO:0039580; P:suppression by virus of host PKR signaling; IEA:UniProtKB-KW.
DR GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0039540; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of host RIG-I activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.420.330; Influenza virus non-structural protein, effector domain; 1.
DR Gene3D; 1.10.287.10; S15/NS1, RNA-binding; 1.
DR HAMAP; MF_04066; INFV_NS1; 1.
DR InterPro; IPR004208; NS1.
DR InterPro; IPR000256; NS1A.
DR InterPro; IPR038064; NS1A_effect_dom-like_sf.
DR InterPro; IPR009068; uS15_NS1_RNA-bd_sf.
DR Pfam; PF00600; Flu_NS1; 1.
DR SUPFAM; SSF143021; Ns1 effector domain-like; 1.
DR SUPFAM; SSF47060; S15/NS1 RNA-binding domain; 1.
PE 3: Inferred from homology;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247, ECO:0000256|RuleBase:RU362113};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200,
KW ECO:0000256|RuleBase:RU362113};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995,
KW ECO:0000256|RuleBase:RU362113};
KW Host mRNA suppression by virus {ECO:0000256|ARBA:ARBA00022557,
KW ECO:0000256|RuleBase:RU362113};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562,
KW ECO:0000256|RuleBase:RU362113};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581,
KW ECO:0000256|RuleBase:RU362113};
KW Inhibition of host innate immune response by virus
KW {ECO:0000256|ARBA:ARBA00022632};
KW Inhibition of host interferon signaling pathway by virus
KW {ECO:0000256|ARBA:ARBA00022830};
KW Inhibition of host PKR by virus {ECO:0000256|ARBA:ARBA00023041};
KW Inhibition of host pre-mRNA processing by virus
KW {ECO:0000256|ARBA:ARBA00022834, ECO:0000256|RuleBase:RU362113};
KW Inhibition of host RIG-I by virus {ECO:0000256|ARBA:ARBA00023090};
KW Inhibition of host RLR pathway by virus {ECO:0000256|ARBA:ARBA00022482};
KW Interferon antiviral system evasion {ECO:0000256|ARBA:ARBA00023258,
KW ECO:0000256|RuleBase:RU362113};
KW Reference proteome {ECO:0000313|Proteomes:UP000129778};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|RuleBase:RU362113};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843};
KW Viral immunoevasion {ECO:0000256|ARBA:ARBA00023280}.
FT REGION 205..230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..230
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 230 AA; 26145 MW; 3691CA90BBBBC07F CRC64;
MDSNTVSSFQ VDCFLWHVRK RFADQELGDA PFLDRLRRDQ KSLRGRGSTL GLDIETATRA
GKQIVERILE EESDEALKMT IASVPDSRYL TDMTLEEMSR DWFMLMPKQK MAGSLCIRMD
QAIMDKDIIL KANFSVIFNR LETLILLRAF TEDGAIVGEI SPLPSLPGHT DEDVKNAIGV
LIGGLEWNDN TVRVSETLQR FAWRSSNEDG RPPLPPKQKR KMARTIESEV
//
