ID D1W6S7_9BACT Unreviewed; 382 AA.
AC D1W6S7;
DT 09-FEB-2010, integrated into UniProtKB/TrEMBL.
DT 09-FEB-2010, sequence version 1.
DT 24-JAN-2024, entry version 53.
DE RecName: Full=dTDP-glucose 4,6-dehydratase {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
DE EC=4.2.1.46 {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
GN Name=rfbB {ECO:0000313|EMBL:EFA91697.1};
GN ORFNames=HMPREF0650_0013 {ECO:0000313|EMBL:EFA91697.1};
OS Hoylesella buccalis ATCC 35310.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Hoylesella.
OX NCBI_TaxID=679190 {ECO:0000313|EMBL:EFA91697.1, ECO:0000313|Proteomes:UP000005283};
RN [1] {ECO:0000313|EMBL:EFA91697.1, ECO:0000313|Proteomes:UP000005283}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35310 {ECO:0000313|EMBL:EFA91697.1,
RC ECO:0000313|Proteomes:UP000005283};
RA Durkin A.S., Madupu R., Torralba M., Methe B., Sutton G., Strausberg R.L.,
RA Nelson K.E.;
RT "Genome Sequence of Prevotella buccalis ATCC 35310.";
RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC Evidence={ECO:0000256|ARBA:ARBA00001539,
CC ECO:0000256|RuleBase:RU004473};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911,
CC ECO:0000256|RuleBase:RU004473};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily.
CC {ECO:0000256|ARBA:ARBA00008178, ECO:0000256|RuleBase:RU004473}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFA91697.1}.
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DR EMBL; ADEG01000073; EFA91697.1; -; Genomic_DNA.
DR RefSeq; WP_004349806.1; NZ_ADEG01000073.1.
DR AlphaFoldDB; D1W6S7; -.
DR STRING; 679190.HMPREF0650_0013; -.
DR eggNOG; COG1088; Bacteria.
DR Proteomes; UP000005283; Unassembled WGS sequence.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01181; dTDP_gluc_dehyt; 1.
DR PANTHER; PTHR43000:SF7; DTDP-D-GLUCOSE 4,6-DEHYDRATASE; 1.
DR PANTHER; PTHR43000; DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU004473, ECO:0000313|EMBL:EFA91697.1};
KW NAD {ECO:0000256|ARBA:ARBA00023027}.
FT DOMAIN 5..351
FT /note="NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF16363"
SQ SEQUENCE 382 AA; 43868 MW; BFC183C94F953725 CRC64;
MKTYLVTGAA GFIGANFIKY LLDKKYKNED IKVIILDALT YAGNLGTIKN NIDNKRCFFV
KGDIRDRALA DKLFAEHDID FVVNFAAESH VDRSIEDPQL FLSVNILGTQ NLMDAARRAW
VTGKDAQGYP TWKAGKRYHQ VSTDEVYGSL GAEGYFSEQT PLCPHSPYSA SKTSADMIVM
AYRDTYRMPV SITRCSNNYG PYHFPEKLIP LIIKNILEGK QLPVYGKGDN VRDWLYVEDH
CKAIDMVVRE GKEGEVYNVG GHNEMKNIDI VKLTIKTIHD MMQQDKDLRK VLKKQEVDEQ
GNIKIDWIND NLITFVADRL GHDQRYAIDP TKIKNDLGWY PETMFADGIV KTIKWNLQHQ
DWINEVTSGD YQKYYEQMYG NR
//
