ID D2PWV7_KRIFD Unreviewed; 3497 AA.
AC D2PWV7;
DT 02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 02-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 82.
DE SubName: Full=Amino acid adenylation domain protein {ECO:0000313|EMBL:ADB35337.1};
GN OrderedLocusNames=Kfla_6335 {ECO:0000313|EMBL:ADB35337.1};
OS Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399).
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Kribbellaceae; Kribbella.
OX NCBI_TaxID=479435 {ECO:0000313|EMBL:ADB35337.1, ECO:0000313|Proteomes:UP000007967};
RN [1] {ECO:0000313|Proteomes:UP000007967}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17836 / JCM 10339 / NBRC 14399
RC {ECO:0000313|Proteomes:UP000007967};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M.,
RA Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D.,
RA Pukall R., Klenk H.-P., Eisen J.A.;
RT "The complete genome of Kribbella flavida DSM 17836.";
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADB35337.1, ECO:0000313|Proteomes:UP000007967}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17836 / JCM 10339 / NBRC 14399
RC {ECO:0000313|Proteomes:UP000007967};
RX PubMed=21304701;
RA Pukall R., Lapidus A., Glavina Del Rio T., Copeland A., Tice H.,
RA Cheng J.-F., Lucas S., Chen F., Nolan M., LaButti K., Pati A., Ivanova N.,
RA Mavrommatis K., Mikhailova N., Pitluck S., Bruce D., Goodwin L., Land M.,
RA Hauser L., Chang Y.-J., Jeffries C.D., Chen A., Palaniappan K., Chain P.,
RA Rohde M., Goeker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA Kyrpides N.C., Klenk H.-P., Brettin T.;
RT "Complete genome sequence of Kribbella flavida type strain (IFO 14399).";
RL Stand. Genomic Sci. 2:186-193(2010).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- SIMILARITY: In the C-terminal section; belongs to the NRP synthetase
CC family. {ECO:0000256|ARBA:ARBA00029443}.
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DR EMBL; CP001736; ADB35337.1; -; Genomic_DNA.
DR RefSeq; WP_012923890.1; NC_013729.1.
DR STRING; 479435.Kfla_6335; -.
DR KEGG; kfl:Kfla_6335; -.
DR eggNOG; COG0318; Bacteria.
DR eggNOG; COG1020; Bacteria.
DR eggNOG; COG2141; Bacteria.
DR eggNOG; COG3321; Bacteria.
DR HOGENOM; CLU_000274_0_0_11; -.
DR OrthoDB; 9808669at2; -.
DR Proteomes; UP000007967; Chromosome.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 3.30.70.3290; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR Gene3D; 3.20.20.30; Luciferase-like domain; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR024011; Biosynth_lucif-like_mOase_dom.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR NCBIfam; TIGR04020; seco_metab_LLM; 1.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 1.
DR Pfam; PF00296; Bac_luciferase; 1.
DR Pfam; PF00668; Condensation; 2.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 1.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF51679; Bacterial luciferase-like; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 4.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 3: Inferred from homology;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000007967};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 574..649
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 672..1086
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1555..1630
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3016..3091
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 553..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1495..1533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1693..1733
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2288..2309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1513..1528
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1693..1713
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 3497 AA; 373170 MW; DF3522DE25129056 CRC64;
MANSSLTRGS QLILTGADPA DAVQALTRAA TEFPAAGVRT PAGLLTYADL LDRARRILTG
LRATGLSSGD PVIVQGLSLD DFFPAFWACL LGGIQPAVIS EQVSPGSPVL DRLRHTWALL
DTPPVLTDRT GAVVLADRGF QPAVVDELQQ SLPADELHRP DPAEVALLML SSGSTGAPKA
AALTHRGLAE FAAGSRRVLD VRPGETMLNW LPVDHSGALL LYHVLPVFTG STNVHLPTAD
VLANPSLWLE KAAQYGVAHA WSPTFGLQLA ADAFAARQDL ELDLSCVRSV VSGGEQIVLP
VVDRFFAATA RAGLGRERFR PAWGMAETAT AITIGALTSQ GGVLRVLKSS LAGDLVLAEP
SVPDADCTTF VAVGPPAPGA TVRVVDDNDE VLPELRIGRL QVRSARITAG YLNNPSATAA
ALPDGEWLRT GDLAFVADGQ VVITGREGDS VVLNGHTVFC HEIEEVVGGV DGLSLRGVGV
CGVPNPGTGT EDLVVFVDDA GTPGEVEPAI RAALFRRLRL TAAEIVRVPA GEFPRTASGK
VRRTELRARY QAGRYSRDSA ASQGGRSAGE DRMAPSVGEV GAVVRRLVAE LAGQPVAVDA
PFYEVGLSSI KLARLRAGLT EQLGIDVPAT TPFEHPTVDA LTHWLTEQLA ASGPAQVEPD
GRADEAGRRV VDRRVAVVGM AARFPGARTV EEFWANLWGG VDSVSTFAAA GGPDHVPVGG
VLEDAETFDA EFFGMSPKEA ALTDAAHRQF LEVAHEVLEN GGYPSAEPGV RIGVYAGTGM
NLYGHQDQPA GAAAADIPTA MQATIGATAD FLATRVAYRL GLTGPAIGVQ TACSTSLVAV
HLAVQALLDD DADLAVAGAA AVRLPQDAGY RHVPGSILSP TGRCRPFDAA ADGTVGGNGV
AAVLLKRLDR ALADGDTVHA VILGSAVNND GTSKVGFTAP SVSGQVDVVR RALERAGVGA
DTISYVEAHG TGTALGDPVE FEALSRVFGA GQRTAKCGLG SVKANVGHLD SCAGLAGLIK
TILMLQHGRL VPTLNLTQPH PDLRVERSPF ELITASGPWR TDGVPRRAGV SALGVGGTNA
HIVLEQPPLE SGRDETPVPQ ATDGQTNGFH PVVVPVSAQS VEALGELSAS LRAYLAARPE
VAVADVATTM GVGRRHHACR RAVVGAEVGE VVAALGEDPG EVVTGPLTFA YSGQGSAYSG
MSEWMYRTFR PAREVFDEIE RVLGVPVLEG EGELGQVALF AHQAAWTEVW RGLGVVPDFV
VGHSLGEYAA LHAAGVLSLT DGALLTARRG ELMQAGMEPG VMVAVGAELA EAEQIAAECG
VEVAAVNGRD RVVLSGAEDA MARAVGVLDG RGVSWRRMGV ERAFHSVMVE PVLGELRAFA
EKVAFRPPRV PVFRGTDGAV LSGVDGRYLV EHARRAVRFD LLMRAVRERG GNRFLDVGPD
AVLSGLGRRA LPGSAWVASQ RKGAEDQFAQ TLAALYRAGA DINWSMVSTG RRIPLPGHPL
ARRRYDRPST PTVPPQTAVP PQPTVPARTP EPMRATEPMQ GVEQMQVQSV GAERAAVLQQ
VRQLVAPSLG MEPDAVPAEA TFLSLGADSL SLMRLVGDLD EVFGVKIPVR RLFDDADTPN
RLVDLLSPSA AVVPQPLQQE SQPVQLQEAP AAQAVDDGGR RELVDRQLRL AERMVDRVTD
LMSEQLAFLN GASPTTQQAS TPMVDSSTAP PATVTHPEAP PQPTPARRNS PHRRTGCDFS
LYFFGDYPDH AAQDKYGLIL KATEYADAHG FHAVWLPERH FHSFGALFPN PSVLAAALAT
RTQRIRLHAG SVVLPLHHPI RVAEEWSVVD NLSGGRAGMC VASGWHARDF VFAPDSYGRH
REELYEHLDT VRRLWAGEAI TATAGNGEST EVRLHPRPLQ DQPPLYAAVV GNPESYKLAA
RNDIGVVTNL MAQSVEDLAA NIALYRSTRA EHGLDPDAGR VVVLLHTYVG DDLEAVRAAA
YRPFCDYLRS SLSLFGQVTN SLGFQIDLDK TPADDVDFLL GQAYQRYCES RALIGTPDSC
ATIVDALLAA GADEIACFVD FGVAPEQVMQ SLTAVDALRA EYDGAAPPRH EGRELTPPER
RIWFLEQLNP GTNRYHEPKA IELRGPLDPV ALRGALQKVA DRHPALRTTF REIDGEPRAF
VGATAPIDCP MVDAEGKDLS SVLRDVRLGE LDPATGPLVR AQLVRFSERH HVLLLVAHHI
VFDSASTPVL ARDLGAYYRS WPDDPDLPAA TDGLHLPAGG DQEPAALEAR KKGDLDFWVE
HLSGAPELQL PADRPRPPRR SGAGSSLTHD LDRRLTEQLT AFAGRTGVTV FSTLLAGLSV
VLSRFSGQRD FVLGTGVSGR GKHAGDAIGM FVDTLPLRVR IPATAAFADV TRDLGLALMD
AVDHRDLPFE DIVAALNPER SAGRNPLFGV AVEFESGSEQ ISFAPPTVSA TLLDLPSERA
PLDLVLYLTY RPDGVQCVVE YDTDLYDEST IQRLLDYLGT TLDLATASTP VAVPDLAVLT
EQDSRLLAQL EGRPAEESTL CLHELFERQV DQHPEALALT GAYGDTTYAE LDRQANRIAA
ELLTRGVRRG ELVALCLPRG PQQIAALLGV LKSGYAYLPL DPTVPVERLR LVVDDADAAL
VVTAEGMPDL GDRPRLRIDD LDAGPVDRPR TDVTPDDLAY CIYTSGSTGT PKGVLVPHRG
TVNHVLQYVA AHPPTRLLQW ATPTFDGSVH EVFTTLAAGQ TLVLIDDASR YDVEALAATI
DRYQVQRIVM PYSAVHSLLA TKPSLPSLRE ILTAGETVRL TAADLEFLAA HPNCVLYNGY
GPTEASIGVT EHRVEPGEIA PPVGRPIPGV RLRLLDAERR PVPMGAVGEI CVEGVCVTDG
YLNRSAETAA AFFAPNSYAT GDLGRWRADG GLEFHGRRDE QVKIRGARIE PGEVRAAVLD
HPLVTDAAVL AVDGELVGYV VGTVGGDELT DHLSAQLPQY LVPRRWVRLD RLPLASTGKL
DRAALPAPEN ARGSATPSTA MEHTLHEVWC KVLGVTAVGV DDSFFALGGH SLLAVRLLNL
VRETAGVELT LTEFFRAPTL RAVAAKAQGD VVVETAPLTF AQRRVLGRHR SRRDASVYNG
ITRVDVTGDL NPATLQAALR TLANRHTALR TRIAADRQEV FAAVPITLPV HELPDDESQI
QAWCVAAAQP AMDPAQAPLW RVCLGRVSAE HWVLVVVVHH LIYDGWSARL FWQELSALYT
GAELAPPTAQ LTDYARWERD TLTGETRTQL EAFWRNELAG ASLRPNLPVD HPRPDVLSGD
GATHHVDLPH ELAARLRARA AEAGTTPYVV IAAAFARWLG DLCGEDQVIL PASSARRSRP
EHDGIIGYLG EAVLVRVRLD APDLLGETAG ALYAALDHEA LPLAEVVRTA LPDQADLPYP
AVLFTVITTP GATLTLPSGE FRTRGFSVPG QARTELYVVF TVTEEAFTLD IEYSTDLFTS
TTIAEWAMSL VTALTGP
//
