ID D2PZ46_KRIFD Unreviewed; 1906 AA.
AC D2PZ46;
DT 02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 02-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN OrderedLocusNames=Kfla_2775 {ECO:0000313|EMBL:ADB31840.1};
OS Kribbella flavida (strain DSM 17836 / JCM 10339 / NBRC 14399).
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Kribbellaceae; Kribbella.
OX NCBI_TaxID=479435 {ECO:0000313|EMBL:ADB31840.1, ECO:0000313|Proteomes:UP000007967};
RN [1] {ECO:0000313|Proteomes:UP000007967}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17836 / JCM 10339 / NBRC 14399
RC {ECO:0000313|Proteomes:UP000007967};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M.,
RA Hauser L., Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D.,
RA Pukall R., Klenk H.-P., Eisen J.A.;
RT "The complete genome of Kribbella flavida DSM 17836.";
RL Submitted (SEP-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ADB31840.1, ECO:0000313|Proteomes:UP000007967}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17836 / JCM 10339 / NBRC 14399
RC {ECO:0000313|Proteomes:UP000007967};
RX PubMed=21304701;
RA Pukall R., Lapidus A., Glavina Del Rio T., Copeland A., Tice H.,
RA Cheng J.-F., Lucas S., Chen F., Nolan M., LaButti K., Pati A., Ivanova N.,
RA Mavrommatis K., Mikhailova N., Pitluck S., Bruce D., Goodwin L., Land M.,
RA Hauser L., Chang Y.-J., Jeffries C.D., Chen A., Palaniappan K., Chain P.,
RA Rohde M., Goeker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA Kyrpides N.C., Klenk H.-P., Brettin T.;
RT "Complete genome sequence of Kribbella flavida type strain (IFO 14399).";
RL Stand. Genomic Sci. 2:186-193(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
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DR EMBL; CP001736; ADB31840.1; -; Genomic_DNA.
DR STRING; 479435.Kfla_2775; -.
DR CAZy; CBM48; Carbohydrate-Binding Module Family 48.
DR CAZy; GH13; Glycoside Hydrolase Family 13.
DR KEGG; kfl:Kfla_2775; -.
DR eggNOG; COG0366; Bacteria.
DR eggNOG; COG1523; Bacteria.
DR eggNOG; COG3209; Bacteria.
DR HOGENOM; CLU_000910_0_0_11; -.
DR Proteomes; UP000007967; Chromosome.
DR GO; GO:0051060; F:pullulanase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11339; AmyAc_bac_CMD_like_2; 1.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR CDD; cd12962; X25_BaPul_like; 3.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR Gene3D; 2.60.40.10; Immunoglobulins; 4.
DR Gene3D; 2.60.40.1130; Rab geranylgeranyltransferase alpha-subunit, insert domain; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011839; Pullul_strch.
DR InterPro; IPR024561; Pullul_strch_C.
DR InterPro; IPR040671; Pullulanase_N2.
DR NCBIfam; TIGR02103; pullul_strch; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF11852; Pullul_strch_C; 1.
DR Pfam; PF17967; Pullulanase_N2; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 2.
DR SUPFAM; SSF81296; E set domains; 2.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000007967};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..33
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 34..1906
FT /note="pullulanase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003034816"
FT DOMAIN 163..623
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 822..848
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1573..1605
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1906 AA; 203293 MW; 3FE19851F9B6CE94 CRC64;
MIRFVMRRAR WLAGVTAVAV VAVGLGTPSA APAGPSAERV ITLAGSLQSE LGCPGDWDPG
CARSTLGTAA PYSKVFDVPA GSYEYKVTVN GGWDENYGAG GVLNGANIPL RIEGPAKLRF
SYDDTSHVVS VQPVDVAGGL TAADRALASP SLRKDLTKER FYFLMADRFA NGDKSNDAGG
LTGDRLSTGL DPADKGFYHG GDLAGVMQKL DYIKSLGTTS IWLTPSFKNR PVQGAGADVS
AGYHGYWITD FTQIDPHLGS NEDMRKLIQL AHGKGMKVFF DIITNHTADV IAYRSGQYGY
VPKSTSPYKD AAGNVFDDKQ YAGGDTFPPL DRNVSFPNVP VFRTPADESV KVPNWLNDPT
LYHNRGDSTF AGESAEYGDF VGLDDLFTEQ PKVRDGMIDV YRTWAEMGID GFRIDTVKHV
NLEFWQKFSP EILKAARAKG SKDFFMFGEV FDADPKFMST YTTAGALQAT LDFGFQQNAV
AFAKGDPSTR LRDFYAADDY YTDADSNAYQ LPTFLGNHDM GRVGTFLKQA GASGQELLQR
DKLAHSLMFL TRGQPVVYYG DEQGFTGAGG DKDARQDMFA TKTAEYADDE VVDGTGTSTI
GSKDRFDVDA PMYRHIAGLQ KLRAKYPALS DGTQVHRYSS NTGGLYAFSR LGADKTEYLV
VSNNATTAKT ATIPTYGSKI FRPVYGGTKS FLTDREGRAR LTQPPLSVTV YQALSKVPHR
SKAPSVFMSS PESGATVGGR AEIAAAVPEN TPVTVTFGYR PVGTTAWQRL GSDDNAPYRV
FHDVSKLAKG TLLEYRAVLK DASGNYSVSG SYGVVGDAPA PGGGGGGGTA PVTQPASVSV
PGDHNSEMGC PADWSPDCDQ AQLTLDPKDK VWKKTSTLPA GEHAYKAAIN KTWDENYGAG
GNANGANISY TAPATPVTFY YEHGRHFATS DAQGPIVTVP GSFQSELGCP GDWAPDCMRP
WLTDQDGDGT YTWSTSELGA GSYEAKVAHG LSFDESYGAG GSPTGGNIAV SVPGDGLVVT
FSYVLATHLL TVTTSKPGAQ PDLEQAKAHW LGRDLLAVPP VPHPERSRWR LHWSPTGSLA
IDADDIGGSS AGLRVDPNGL PAAVLAKFPA LKGYTALRLG RVDAESMLTG QLGLAQYDDA
GRLLDATGVQ IPGVLDDLYG AAATGRSYGV TWHGGVPRFT LWAPTAQKVA LLVGAQRIPM
RRTSDGSWIA QGRRSWQNAP YRYEVTVFAP STGKVETNLV TDPYSVALTT DSTHSVAVDL
DDPAGKPALW ARTPAPKLAR PVDSTIYELH VRDFSIDDPT VPAAHRGSYL AFADEGAGTK
HLRKLAQAGL NTVHLLPTFD IASIPEGTQE KPACDLKALP PDSEQQQACV TAVAAKDGFN
WGYDPYHWLA PEGSYAVRKD GLSRVAEFRT MVGGLHRSGL RVVLDQVFNH TPAAGQAPTS
VLDKVVPGYY QRLNATGGVE TSTCCSNIAT EHAMAEKIMV DGTVSWARNY RVDGFRFDLM
GHHSKANMLK VRAALDRLTL AKDGVDGRQV FLYGEGWNFG EVANDALFEQ ARQGNLGGTG
IATFSDRLRD AVRGGGPFDE DPRVQGFGSG AASDPNGAPV NGSPADRAKR LAHDTDLTQL
GLAGNLRAFT FRSAETGAVV RGDAVDYNGA PAGYADQPGE VITYVDAHDN ETLWDALTYK
LPTGLPMADR VRMNTLSLAT TALAQTPSFW HAGADLLRSK SLDRNSYDSG DWFNTLDWTG
ADNGFGHGLP PKPDNEAKWP FMKPLLGNPA LKPSAADVGT ASAAAADLLK LRFSTPLFRL
GSADLVNQKV GFPLSGTPAA VPGVVTMRID DTVGPDVDPA LKGLVVVFNS TGSTVSQQIP
GLTGATLSLS PVQAGGADPV VKQSTWTAAS GTVSVPARTV AVLIQR
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