ID D2SA75_GEOOG Unreviewed; 1268 AA.
AC D2SA75;
DT 02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 02-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 80.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
DE AltName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273};
GN OrderedLocusNames=Gobs_3285 {ECO:0000313|EMBL:ADB75890.1};
OS Geodermatophilus obscurus (strain ATCC 25078 / DSM 43160 / JCM 3152 / KCC
OS A-0152 / KCTC 9177 / NBRC 13315 / NRRL B-3577 / G-20).
OC Bacteria; Actinomycetota; Actinomycetes; Geodermatophilales;
OC Geodermatophilaceae; Geodermatophilus.
OX NCBI_TaxID=526225 {ECO:0000313|EMBL:ADB75890.1, ECO:0000313|Proteomes:UP000001382};
RN [1] {ECO:0000313|EMBL:ADB75890.1, ECO:0000313|Proteomes:UP000001382}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25078 / DSM 43160 / JCM 3152 / KCC A-0152 / KCTC 9177 /
RC NBRC 13315 / NRRL B-3577 / G-20 {ECO:0000313|Proteomes:UP000001382};
RX PubMed=21304698;
RA Ivanova N., Sikorski J., Jando M., Munk C., Lapidus A., Glavina Del Rio T.,
RA Copeland A., Tice H., Cheng J.-F., Lucas S., Chen F., Nolan M., Bruce D.,
RA Goodwin L., Pitluck S., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA Palaniappan K., Land M., Hauser L., Chang Y.-J., Jeffries C.D., Meincke L.,
RA Brettin T., Detter J.C., Detter J.C., Rohde M., Goeker M., Bristow J.,
RA Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.-P.;
RT "Complete genome sequence of Geodermatophilus obscurus type strain (G-
RT 20).";
RL Stand. Genomic Sci. 2:158-167(2010).
RN [2] {ECO:0000313|Proteomes:UP000001382}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25078 / DSM 43160 / JCM 3152 / KCC A-0152 / KCTC 9177 /
RC NBRC 13315 / NRRL B-3577 / G-20 {ECO:0000313|Proteomes:UP000001382};
RG US DOE Joint Genome Institute (JGI-PGF);
RA Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N.,
RA Munk A.C., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L.,
RA Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Jando M.,
RA Schneider S., Klenk H.-P., Eisen J.A.;
RT "The complete genome of Geodermatophilus obscurus DSM 43160.";
RL Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|ARBA:ARBA00007391}.
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DR EMBL; CP001867; ADB75890.1; -; Genomic_DNA.
DR AlphaFoldDB; D2SA75; -.
DR STRING; 526225.Gobs_3285; -.
DR KEGG; gob:Gobs_3285; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_4_1_11; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000001382; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000001382};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT REGION 81..125
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1240..1268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1254..1268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1268 AA; 136656 MW; 7F6501F2FC9E7D85 CRC64;
MRADPFVHLH VASGYSMQYG ANHPADLVER AAEHGMRALA LTDRDGLYGA VKFALACRAA
DVRPLFGVDL AVTPSVDTTV PPALAPVVGG AHSAQRARSR RPGAPDDAPG RAGRRSPARG
GASVDPRLPR VTFLARDGAG WRSLCRLTTA THLRGTRGEP VSSLALAAEH APGLVALLGP
DSPVGRALAA GRADVASARL AAWRAVFGPG SLVLEVVHHR GRGDRSRAQA LLRFAAAEGV
PAVLTNAVRY VDALDAPTAD VLDAARRLVP LDARHVDRRT AEGHLKSGKE MAEVAEDLVG
PDRDAVLQLL ERTARLAEQC AVDVRADLGV GSVRYPELEV VTTPAEQGMG PSAVLRARCE
AGLGRRGMPR TAEVARRLDD ELEVIDVLGY PSYFLTVADV VDLIKSLRVR AAARGSGAGS
LVTYLLGISD VDPIRYGLLM ERFLSPLRHQ LPDVDIDVES ARRMEVYDAV LDRFGAHRVS
CISMMDTYRV RHAIRDVGAA LGLPPAEVDA VAKAFPHIRA NQVHAALKDL PELRASRLGS
RRGGGTGDLD LLFDLVARLD GLPRHIALHP CGVLLSDATL LDRTPVENSY LGYPMSQFDK
DDVEELGLLK LDLLGIRMQS AIAHALDEVA RVDGETVDID AVPRDDPTTF ELIRSTRTLG
MFQIESPGQR ELVGKFGPET FEDIVIDISL FRPGPVKSDM VTPFLLARNG WREPVYPHPD
LAPYLEQTAG VVVFHEQVLQ IVAQMTGCDL AQADEARRAL GDRELHPEVR AWFVPEAVQR
YPVEVVEQVW QVLVAFGSFG FCKAHAAAFA LPTYQSAWLK THHPAAFLAG VLTHDPGMYP
KRLILDDARN FGVRVLGLDV NASAGTYRVE RLEADPDAGP EVETAGGWAR PSWMPVAMPD
PSRYGIRLSL SDVKGICAEE VERVVAGQPY RSLTDFWHRA SVSRPVVERL VLAGGFDSLY
GFGVRDREAG RPTRGRRQVT RRDLLLQIGE LDRWSRSGAK AATTGQLSLD LMGLEMEGTE
LPGEEAEEPG LGWAEGSGLP ELTDAELVRA ELEVLGLDAS RHVVDFYRPF LAAIGAVLAG
ELLGSRSGAE VLVAGVKVAT QTPPIRSGRR VVFVTLDDGT GCTDSTFFED AQGPYASTVF
HSWLLVIRGV LRRTGPRGVS LRATGAWELP ALYEAWETGG LEAVAEQMAA SGGYTEEAIA
GAAASRGSRP VVVKPVLVHP TGFRMSPYAD IKPAGEDAMT VARRDATRRG TSSAPRKLWH
SSPGSSGH
//
