ID   D2V9J7_NAEGR            Unreviewed;       436 AA.
AC   D2V9J7;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 48.
DE   SubName: Full=Predicted protein {ECO:0000313|EMBL:EFC46600.1};
GN   ORFNames=NAEGRDRAFT_65467 {ECO:0000313|EMBL:EFC46600.1};
OS   Naegleria gruberi (Amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5762 {ECO:0000313|Proteomes:UP000006671};
RN   [1] {ECO:0000313|EMBL:EFC46600.1, ECO:0000313|Proteomes:UP000006671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEG-M {ECO:0000313|EMBL:EFC46600.1,
RC   ECO:0000313|Proteomes:UP000006671};
RX   PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA   Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA   Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA   Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA   Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT   "The genome of Naegleria gruberi illuminates early eukaryotic
RT   versatility.";
RL   Cell 140:631-642(2010).
CC   -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC       family. Dihydroflavonol-4-reductase subfamily.
CC       {ECO:0000256|ARBA:ARBA00023445}.
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DR   EMBL; GG738858; EFC46600.1; -; Genomic_DNA.
DR   RefSeq; XP_002679344.1; XM_002679298.1.
DR   AlphaFoldDB; D2V9J7; -.
DR   STRING; 5762.D2V9J7; -.
DR   EnsemblProtists; EFC46600; EFC46600; NAEGRDRAFT_65467.
DR   GeneID; 8859838; -.
DR   VEuPathDB; AmoebaDB:NAEGRDRAFT_65467; -.
DR   eggNOG; KOG1502; Eukaryota.
DR   InParanoid; D2V9J7; -.
DR   OrthoDB; 846192at2759; -.
DR   Proteomes; UP000006671; Unassembled WGS sequence.
DR   GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0006694; P:steroid biosynthetic process; IEA:InterPro.
DR   CDD; cd08958; FR_SDR_e; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR10366:SF564; 3BETA_HSD DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR10366; NAD DEPENDENT EPIMERASE/DEHYDRATASE; 1.
DR   Pfam; PF01073; 3Beta_HSD; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006671}.
FT   DOMAIN          46..314
FT                   /note="3-beta hydroxysteroid dehydrogenase/isomerase"
FT                   /evidence="ECO:0000259|Pfam:PF01073"
SQ   SEQUENCE   436 AA;  49424 MW;  323071C7E9008A27 CRC64;
     MGTNYPEDYS QQHHITTINQ QQELATNNME ETSLPLKENS KRKYCVIGGS GFLGSHLVMT
     LLRRGHAVNL TVRNLGKVEN YEFIKKAACQ EGLDDNLQFF QADLAKDGSF YNAFLDCDGV
     FATACPLDDA LTRNSSDPKS ELFDPIVNGV KNVVRECVKI RKEQNERNDS RRLKRLIFTS
     SITAVSTDAL WDYKSKSWHY FDEAEWNDKA SLTQCTYAYC KTMSEREAFE LLKGEFSNTQ
     LEIAAVLPST MFGPSIGIRK NIGMQPIISS IVDIPAHVRL FCPSVDVRDV ALAHCLVMEK
     TSKPVNDQSN GFYIDHDYLA SNQERVIVHN NTICTRDICQ AISKYPQALQ ILEKKKLKLP
     TQIPFLENTI GDKILLLGAY LTGPKPQYDL LRGMVGKELH FSNNYVKGKY GLEFTDLDQT
     ILETVMHILE HDMHKL
//