ID D2VA07_NAEGR Unreviewed; 519 AA.
AC D2VA07;
DT 02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT 02-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 4 {ECO:0000256|RuleBase:RU364141};
DE AltName: Full=Mediator complex subunit 4 {ECO:0000256|RuleBase:RU364141};
GN Name=MED4 {ECO:0000256|RuleBase:RU364141};
GN ORFNames=NAEGRDRAFT_47850 {ECO:0000313|EMBL:EFC46345.1};
OS Naegleria gruberi (Amoeba).
OC Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC Vahlkampfiidae; Naegleria.
OX NCBI_TaxID=5762 {ECO:0000313|Proteomes:UP000006671};
RN [1] {ECO:0000313|EMBL:EFC46345.1, ECO:0000313|Proteomes:UP000006671}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NEG-M {ECO:0000313|EMBL:EFC46345.1,
RC ECO:0000313|Proteomes:UP000006671};
RX PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT "The genome of Naegleria gruberi illuminates early eukaryotic
RT versatility.";
RL Cell 140:631-642(2010).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000256|RuleBase:RU364141}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000256|RuleBase:RU364141}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU364141}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC {ECO:0000256|ARBA:ARBA00009626, ECO:0000256|RuleBase:RU364141}.
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DR EMBL; GG738859; EFC46345.1; -; Genomic_DNA.
DR RefSeq; XP_002679089.1; XM_002679043.1.
DR AlphaFoldDB; D2VA07; -.
DR EnsemblProtists; EFC46345; EFC46345; NAEGRDRAFT_47850.
DR GeneID; 8859462; -.
DR VEuPathDB; AmoebaDB:NAEGRDRAFT_47850; -.
DR InParanoid; D2VA07; -.
DR OrthoDB; 5485411at2759; -.
DR Proteomes; UP000006671; Unassembled WGS sequence.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR CDD; cd00590; RRM_SF; 1.
DR InterPro; IPR019258; Mediator_Med4.
DR InterPro; IPR035979; RBD_domain_sf.
DR Pfam; PF10018; Med4; 1.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|RuleBase:RU364141};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364141};
KW Reference proteome {ECO:0000313|Proteomes:UP000006671};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU364141};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU364141}.
FT REGION 1..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 355..382
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 13..51
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..72
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 519 AA; 58173 MW; E256EB1A578D5B43 CRC64;
MTKNRKNNKQ QKPAAGNKKE EEKVDTNLMV DEVPEKSQQG VKRKNEKVTS SGEEPTLESL
TKKAKKETND TEEYINATQD PTFTGKITTN SSKIYKNILL ISNLALSVDE IVLKGCMNEI
AEASGNSAQK LKEILIFQKN LSSKESEALA SNALRNAQQK NTNNKQATLI QGPKAAIVIF
EKEFANAQVF EDYNGAIIEG IPINIRFACE NDKACLMVVK GAPITEEELW SYVEECGSLV
SIESSKSNKN QFMILFKEWR SVRECLLYNG ADVHGAPCRI FACMFPAPTN HSSKFKGGNK
ALCAAISKGT RTLTGETAKS KALVAMIKNE IMLQTELLIE KDKELKTLLL ELVENQKFQR
KIDQTKQILD EKEYKIAKLT EALETVHSKL GNALHQSSPI VKSIKESKNL NLDEVFSYSH
FISKVTSEQP ALKESDMQLA PFPAPEIWPL SLLYPSTLRL YKTKYTEKLN AEKEQEEQKI
PRIDFDKFQY ETNAAVPTTQ KAKISLFGDD SDDSDDSDD
//
