ID   D2VSR9_NAEGR            Unreviewed;       942 AA.
AC   D2VSR9;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 47.
DE   RecName: Full=RNA-dependent RNA polymerase {ECO:0000256|RuleBase:RU363098};
DE            EC=2.7.7.48 {ECO:0000256|RuleBase:RU363098};
GN   ORFNames=NAEGRDRAFT_72038 {ECO:0000313|EMBL:EFC40243.1};
OS   Naegleria gruberi (Amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5762 {ECO:0000313|Proteomes:UP000006671};
RN   [1] {ECO:0000313|EMBL:EFC40243.1, ECO:0000313|Proteomes:UP000006671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEG-M {ECO:0000313|EMBL:EFC40243.1,
RC   ECO:0000313|Proteomes:UP000006671};
RX   PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA   Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA   Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA   Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA   Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT   "The genome of Naegleria gruberi illuminates early eukaryotic
RT   versatility.";
RL   Cell 140:631-642(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000256|ARBA:ARBA00024517,
CC         ECO:0000256|RuleBase:RU363098};
CC   -!- SIMILARITY: Belongs to the RdRP family. {ECO:0000256|ARBA:ARBA00005762,
CC       ECO:0000256|RuleBase:RU363098}.
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DR   EMBL; GG738894; EFC40243.1; -; Genomic_DNA.
DR   RefSeq; XP_002672987.1; XM_002672941.1.
DR   AlphaFoldDB; D2VSR9; -.
DR   EnsemblProtists; EFC40243; EFC40243; NAEGRDRAFT_72038.
DR   GeneID; 8860062; -.
DR   VEuPathDB; AmoebaDB:NAEGRDRAFT_72038; -.
DR   eggNOG; KOG0988; Eukaryota.
DR   InParanoid; D2VSR9; -.
DR   OrthoDB; 2735303at2759; -.
DR   Proteomes; UP000006671; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0031047; P:regulatory ncRNA-mediated gene silencing; IEA:UniProt.
DR   InterPro; IPR007855; RNA-dep_RNA_pol_euk-typ.
DR   PANTHER; PTHR23079; RNA-DEPENDENT RNA POLYMERASE; 1.
DR   PANTHER; PTHR23079:SF55; RNA-DIRECTED RNA POLYMERASE; 1.
DR   Pfam; PF05183; RdRP; 1.
PE   3: Inferred from homology;
KW   Nucleotidyltransferase {ECO:0000256|RuleBase:RU363098};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006671};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|RuleBase:RU363098};
KW   RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW   ECO:0000256|RuleBase:RU363098};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363098}.
SQ   SEQUENCE   942 AA;  109669 MW;  6CB40F070E1A3AAF CRC64;
     MRDEDLQTIT GWEDWNVLGF GKNVWKIVVK IDNVQYSELS AKISYMCDST MDLAKNPLEY
     LMKRYRYFAR ELELFESQAN QTLFQSLFNS NVAGGYEQFF GEFYPLVCHV KNRWKKMEGN
     NGKYAIIGDG TNRYSILNSF SRLARILMSL NLGKCLMVVK FDFTSKEDLK ALSECKKEYC
     LSYGIVHEGH KYCLLGTSNS GLKGDSCIFY NSTLLNLEIL KQMLGTFEKA EEHGPRKVAS
     RVGLSMGTST PSIVEWKQDD IQIISDDIAV TDGAGMAVED FFEQVLENTY SVPYFFMDKL
     FESDTKQGRL FEKNFKNISA MQVRILGAKG VIQKVNKSMW AELLKKHKLP SNTKVILRNS
     MVKFEGNYSI YNKIDILNVA RVHGGNINRN IMSCLLQMTT NEEEMEKLFK NFYETQLMEI
     VENKDNPEYL CNVLLSQSGE TSQESIARDL IMAEHSLDNY FISSVVKDNI ESKFESMVSD
     KLSMKCKHPG FLSGMGVYDE SSTLKHDEVH IPCSKLIRSL DYAKIDKVLV YRNPLAYEGD
     IQCLKLVKHP PTEFLQSRRD VIVFGHNPNY KKVPEPLCYM AGGDLDGDLY EIIFDPEIVK
     LFDQNLKPKL NYNDGAATNK VTKTVKNSLS MEDVRKTVLE MMEQSSKISE VYLKTMCWRE
     DSSKVGIDIK LKLSKMYHDS IDTIKGSAFN AKQLYEIPKK DSPKWMELCK LKITDQSHIE
     LDTFEARMFD LIRNIFNRMV FSDTKQLDTV FENPSEIYSS EWQRWKDEFK EAYSQWRLYW
     STRKNDEIVL SDKFSAHKQK LFESIKDCLE EDKINWEKVF IKHDGDSLDK LNHKAAYWLF
     HTYNQYIEED DNEFNLEERF KSLIRSPFVY TCCLFNLNCF KLFNNQEEKM PHIVSPRFTI
     RIKQSRNEKV SKTILLECPL NNYFLDLSYC FPLSKEREFP SF
//