ID   D2W090_NAEGR            Unreviewed;       746 AA.
AC   D2W090;
DT   02-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   02-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 56.
DE   RecName: Full=Acylamino-acid-releasing enzyme {ECO:0000256|ARBA:ARBA00018421};
DE            EC=3.4.19.1 {ECO:0000256|ARBA:ARBA00012917};
GN   ORFNames=NAEGRDRAFT_74773 {ECO:0000313|EMBL:EFC37532.1};
OS   Naegleria gruberi (Amoeba).
OC   Eukaryota; Discoba; Heterolobosea; Tetramitia; Eutetramitia;
OC   Vahlkampfiidae; Naegleria.
OX   NCBI_TaxID=5762 {ECO:0000313|Proteomes:UP000006671};
RN   [1] {ECO:0000313|EMBL:EFC37532.1, ECO:0000313|Proteomes:UP000006671}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEG-M {ECO:0000313|EMBL:EFC37532.1,
RC   ECO:0000313|Proteomes:UP000006671};
RX   PubMed=20211133; DOI=10.1016/j.cell.2010.01.032;
RA   Fritz-Laylin L.K., Prochnik S.E., Ginger M.L., Dacks J.B., Carpenter M.L.,
RA   Field M.C., Kuo A., Paredez A., Chapman J., Pham J., Shu S., Neupane R.,
RA   Cipriano M., Mancuso J., Tu H., Salamov A., Lindquist E., Shapiro H.,
RA   Lucas S., Grigoriev I.V., Cande W.Z., Fulton C., Rokhsar D.S., Dawson S.C.;
RT   "The genome of Naegleria gruberi illuminates early eukaryotic
RT   versatility.";
RL   Cell 140:631-642(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Cleavage of an N-acetyl or N-formyl amino acid from the N-
CC         terminus of a polypeptide.; EC=3.4.19.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000721};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR   EMBL; GG738917; EFC37532.1; -; Genomic_DNA.
DR   RefSeq; XP_002670276.1; XM_002670230.1.
DR   AlphaFoldDB; D2W090; -.
DR   STRING; 5762.D2W090; -.
DR   MEROPS; S09.004; -.
DR   EnsemblProtists; EFC37532; EFC37532; NAEGRDRAFT_74773.
DR   GeneID; 8857405; -.
DR   VEuPathDB; AmoebaDB:NAEGRDRAFT_74773; -.
DR   eggNOG; KOG2100; Eukaryota.
DR   InParanoid; D2W090; -.
DR   OMA; QEIATPF; -.
DR   OrthoDB; 2877590at2759; -.
DR   Proteomes; UP000006671; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   Gene3D; 2.120.10.30; TolB, C-terminal domain; 1.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR045550; AARE_N.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR001375; Peptidase_S9.
DR   PANTHER; PTHR42776:SF4; ACYLAMINO-ACID-RELEASING ENZYME; 1.
DR   PANTHER; PTHR42776; SERINE PEPTIDASE S9 FAMILY MEMBER; 1.
DR   Pfam; PF19283; APEH_N; 1.
DR   Pfam; PF00326; Peptidase_S9; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   SUPFAM; SSF82171; DPP6 N-terminal domain-like; 1.
PE   4: Predicted;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006671}.
FT   DOMAIN          12..270
FT                   /note="Acylamino-acid-releasing enzyme N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF19283"
FT   DOMAIN          529..744
FT                   /note="Peptidase S9 prolyl oligopeptidase catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF00326"
SQ   SEQUENCE   746 AA;  84012 MW;  19B4647B475AC896 CRC64;
     MSLNVDSQTA SRVSQLYEDL KTIPTLSGGL ILPSSCIEIN CTVSDHQNDK NISLSKQYQL
     VNDHQVVGSP YPSITPADIH LVSTSPSGKR RAVFKCIASD GKPNANTAAL DYQIEIYSNQ
     VLQLRILVKD IHGKVFTDET FGRVSWSSCE NKLLYVAEMK QELVKPYWEK YSRTNDKLPE
     NDNPYQYKYD YEAKEDWGEQ ILLKTPHIYE LDILVKKVTH IDLIPATDSA GSPHYLPGST
     NFVYTAFERP VRKLGIRFCV QRDAKLFYCD RISVKQIASE YRGPRRPVFS PCGTKLAFLA
     IENSVNYHNS TSKLVVVNWT TEGAKDSQVI VDIVDEVKCN DEIEAMNTFP GLYNIDLPLS
     CWSSDSRYIV MNSTWRSVQS IVVIDTQKTS NNVRRLFNSN TYESYVLLSH EPQSKKVLYY
     KTTPTMPYQI YVGKLDYDSL EVSNITLIED ALSLINTELN DSGIKEKLES ISWKVLHIPI
     EKGSKVYMDC ILYIPKGPKS FVNGSGSDKH SLLLIPHGGP HGSCSTIFAA RFIYFALCGY
     AVLLLNYRGS VGFSQSFAGC LPGNIGDMDV KDCYNSYRYI LDENVIPVDE NSVSVYGGSH
     GGFLSGHLVG QYPSQFKAGI LLNPVINLAT IFSESDIPDW CYNESLGNDY YDEACVTKEM
     LTQMYDRSPI AHVSNVTAAV LLLVGEVDRR VPKEQPREFY HSLRLLGKCK EARMLVYPEN
     DHPIAKPKDD FDSMVSSALF LYKHSN
//