ID VPY_MEDTR Reviewed; 541 AA.
AC D3J162;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 1.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Protein VAPYRIN {ECO:0000303|PubMed:19912567};
DE Short=MtVpy {ECO:0000303|PubMed:19912567};
DE AltName: Full=Protein HERMES {ECO:0000303|PubMed:21223389};
GN Name=VPY {ECO:0000303|PubMed:19912567};
GN Synonyms=FNB4 {ECO:0000303|PubMed:21223389},
GN HMS {ECO:0000303|PubMed:21223389};
GN OrderedLocusNames=MTR_6g027840 {ECO:0000312|EMBL:KEH25576.1};
GN ORFNames=MtrunA17_Chr6g0461061 {ECO:0000312|EMBL:RHN50766.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE,
RP DEVELOPMENTAL STAGE, INDUCTION BY GLOMUS VERSIFORME AND RHIZOBIA BACTERIA,
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Jemalong A17;
RX PubMed=19912567; DOI=10.1111/j.1365-313x.2009.04072.x;
RA Pumplin N., Mondo S.J., Topp S., Starker C.G., Gantt J.S., Harrison M.J.;
RT "Medicago truncatula Vapyrin is a novel protein required for arbuscular
RT mycorrhizal symbiosis.";
RL Plant J. 61:482-494(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE,
RP INDUCTION BY SINORHIZOBIUM MELILOTI NOD FACTORS, TISSUE SPECIFICITY,
RP INDUCTION BY MYCORRHIZAL FUNGI AND NITROGEN-FIXING RHIZOBIAL BACTERIA, AND
RP SUBCELLULAR LOCATION.
RX PubMed=21223389; DOI=10.1111/j.1365-313x.2010.04415.x;
RA Murray J.D., Muni R.R., Torres-Jerez I., Tang Y., Allen S., Andriankaja M.,
RA Li G., Laxmi A., Cheng X., Wen J., Vaughan D., Schultze M., Sun J.,
RA Charpentier M., Oldroyd G., Tadege M., Ratet P., Mysore K.S., Chen R.,
RA Udvardi M.K.;
RT "Vapyrin, a gene essential for intracellular progression of arbuscular
RT mycorrhizal symbiosis, is also essential for infection by rhizobia in the
RT nodule symbiosis of Medicago truncatula.";
RL Plant J. 65:244-252(2011).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [6]
RP INTERACTION WITH EX70I, AND SUBCELLULAR LOCATION.
RX PubMed=26234213; DOI=10.1016/j.cub.2015.06.075;
RA Zhang X., Pumplin N., Ivanov S., Harrison M.J.;
RT "EXO70I is required for development of a sub-domain of the periarbuscular
RT membrane during arbuscular mycorrhizal symbiosis.";
RL Curr. Biol. 25:2189-2195(2015).
RN [7]
RP INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
CC -!- FUNCTION: Required for arbuscular mycorrhizal (AM) symbiosis with AM
CC fungi (e.g. Glomus versiforme and Gigaspora gigantea) both during
CC fungal passage across root epidermis and for arbuscule formation in
CC cortical cells; this symbiosis promotes phosphorus (P) and copper (Cu)
CC uptake (PubMed:19912567, PubMed:21223389). Essential for infection by
CC symbiotic nitrogen-fixing rhizobial bacteria (e.g. Sinorhizobium
CC meliloti) leading to the formation of root nodules (PubMed:21223389).
CC {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:21223389}.
CC -!- SUBUNIT: Interacts with EX70I at the periarbuscular membrane (PAM)
CC around the arbuscule hyphal tips. {ECO:0000269|PubMed:26234213}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19912567,
CC ECO:0000269|PubMed:21223389}. Nucleus {ECO:0000269|PubMed:19912567,
CC ECO:0000269|PubMed:21223389}. Cell membrane
CC {ECO:0000269|PubMed:26234213}; Peripheral membrane protein
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=In cells containing
CC arbuscular mycorrhizal (AM) fungal hyphae and arbuscules, accumulates
CC in small puncta that move through the cytoplasm, likely mobile
CC spherical structures that are associated with the tonoplast referred to
CC as 'tonospheres' (PubMed:19912567). Present in cytoplasmic strands
CC below hyphopodia (PubMed:19912567). Observed associated with EX70I in
CC zones adjacent to the periarbuscular membrane (PAM) around the
CC arbuscule hyphal tips (PubMed:26234213). Occasionally observed in the
CC nucleus of cells containing fungal hyphae (PubMed:19912567).
CC {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:26234213}.
CC -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:19912567,
CC ECO:0000269|PubMed:21223389}.
CC -!- DEVELOPMENTAL STAGE: First observed in root vascular tissues and root
CC caps (PubMed:19912567). During arbuscular mycorrhizal (AM) symbiosis
CC with (AM) fungi (e.g. Glomus versiforme), accumulates transiently in
CC the root epidermis and outer cortical cells coincident with hyphal
CC penetration (hyphopodia), and later present in cortex cells during
CC arbuscule formation (PubMed:19912567). {ECO:0000269|PubMed:19912567}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal (AM) fungi (e.g. Glomus
CC versiforme and Gigaspora gigantea) (PubMed:19912567, PubMed:21223389,
CC PubMed:26511916). Induced in roots (including root hairs) by rhizobial
CC bacteria (e.g. Sinorhizobium meliloti) synthesized Nod factors in a
CC NFP, DMI1 and DMI3-dependent manner (PubMed:21223389). Also observed in
CC root nodules that arise from symbiotic associations with nitrogen-
CC fixing Rhizobia (PubMed:19912567, PubMed:21223389).
CC {ECO:0000269|PubMed:19912567, ECO:0000269|PubMed:21223389,
CC ECO:0000269|PubMed:26511916}.
CC -!- DISRUPTION PHENOTYPE: Impaired arbuscular mycorrhiza (AM) fungi (e.g.
CC Glomus versiforme and Gigaspora gigantea) passage across root epidermis
CC and abolished arbuscule formation in root cortex during AM symbiosis,
CC thus leading to aborted hyphopodia and no arbuscules (PubMed:19912567,
CC PubMed:21223389). Reduced accumulation of PT4 in AM roots
CC (PubMed:19912567). Abnormal nitrogen-fixing rhizobial bacteria (e.g.
CC Sinorhizobium meliloti) infection threads and fewer small white root
CC nodules, but normal calcium spiking in root hairs in response to Nod
CC factors (PubMed:21223389). {ECO:0000269|PubMed:19912567,
CC ECO:0000269|PubMed:21223389}.
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DR EMBL; GQ423209; ADC33495.1; -; Genomic_DNA.
DR EMBL; FJ795648; ACX94227.1; -; Genomic_DNA.
DR EMBL; CM001222; KEH25576.1; -; Genomic_DNA.
DR EMBL; PSQE01000006; RHN50766.1; -; Genomic_DNA.
DR RefSeq; XP_013451548.1; XM_013596094.1.
DR AlphaFoldDB; D3J162; -.
DR SMR; D3J162; -.
DR STRING; 3880.D3J162; -.
DR EnsemblPlants; KEH25576; KEH25576; MTR_6g027840.
DR GeneID; 25495843; -.
DR Gramene; KEH25576; KEH25576; MTR_6g027840.
DR KEGG; mtr:25495843; -.
DR HOGENOM; CLU_000134_53_1_1; -.
DR OrthoDB; 2385921at2759; -.
DR Proteomes; UP000002051; Chromosome 6.
DR Proteomes; UP000265566; Chromosome 6.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0085042; C:periarbuscular membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0009877; P:nodulation; IMP:UniProtKB.
DR GO; GO:0002237; P:response to molecule of bacterial origin; IEP:UniProtKB.
DR GO; GO:0009609; P:response to symbiotic bacterium; IMP:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 4.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR000535; MSP_dom.
DR InterPro; IPR008962; PapD-like_sf.
DR PANTHER; PTHR24178; MOLTING PROTEIN MLT-4; 1.
DR PANTHER; PTHR24178:SF41; MSP DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF12796; Ank_2; 4.
DR Pfam; PF00635; Motile_Sperm; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 8.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF49354; PapD-like; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 7.
DR PROSITE; PS50202; MSP; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cell membrane; Cytoplasm; Membrane; Nucleus;
KW Reference proteome; Repeat.
FT CHAIN 1..541
FT /note="Protein VAPYRIN"
FT /id="PRO_0000450029"
FT DOMAIN 4..138
FT /note="MSP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00132"
FT REPEAT 176..205
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 209..238
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 242..271
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REPEAT 275..304
FT /note="ANK 4"
FT /evidence="ECO:0000255"
FT REPEAT 309..338
FT /note="ANK 5"
FT /evidence="ECO:0000255"
FT REPEAT 342..372
FT /note="ANK 6"
FT /evidence="ECO:0000255"
FT REPEAT 374..392
FT /note="ANK 7"
FT /evidence="ECO:0000255"
FT REPEAT 396..425
FT /note="ANK 8"
FT /evidence="ECO:0000255"
FT REPEAT 429..458
FT /note="ANK 9"
FT /evidence="ECO:0000255"
FT REPEAT 462..491
FT /note="ANK 10"
FT /evidence="ECO:0000255"
SQ SEQUENCE 541 AA; 59134 MW; 8E66558DE0998BEA CRC64;
MDRLIKLDPS NIVLIRVEEG QKCLGKITLN NVMYTMPVAF RIQPLIKTRY TIKPQSGIIS
PLASLVIEIT YHPPQQQGSN NLPHSFPFSD DSFLLHSVLA PGAAIKEPSS MFDSVPSDWF
TTKKKQVFID SAIKVMFVGS QILTQLVEDG NSMDDIREVL EKSDPLWESV NSKDSQGQTL
LHLAISKTRP DLVQLILEFK PDIEAINSVG STPLEAASSS GESLIVELLL AHKANTEGSE
SSVFRPIHHA SREGHMEILR LLLLKGARVD SLTKDGNTSL HLAVEEKRRD CARLLLANGA
RTDVRNMREG DTPLHIAAAN GDENMVKLLL HKGATKYVRN KLGKTAFDVA AENGHSRLFD
ALRLGDNLCA AARKGEVRTI QKVLESGGVI NGRDQNGWTS LHRAAFKGRM DAVRFLVEKG
IDLDAKDEDG YTALHCAAES GHADVTEFLV KKGADVEART NKGVSALQIV ESLNYVGITR
ILVNGGASRE GLGEKPPSAP SKIPFGRKVE SGSVMTMKKK MSSRTRALRG SFDHSMPLAV
L
//
